Development of cell chip for examination of radiobiological damage

• Project/Area Number: 21651021
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Osaka University
• Principal Investigator: IIDA Toshiyuki 大阪大学, 大学院・工学研究科, 教授 (60115988)
• Co-Investigator(Kenkyū-buntansha): KATO Yushi 大阪大学, 大学院・工学研究科, 准教授 (40224547) ; SATO Fuminobu 大阪大学, 大学院・工学研究科, 助教 (40332746)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,480,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 2009: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: 応答 / 細胞損傷 / DNA損傷 / 放射線応答細胞チップ / 細胞放射線影響 / アルファ粒子 / 培養細胞 / 熱中性子捕獲反応 / AmBe中性子線源 / 中性子

Research Abstract

A cell chip was developed for the examination of biological damage of cells irradiated by high-energyα-rays. A CR-39 track detector was employed as a chip substrate to identify high-energy charged particles traversing target cells. HeLa cancer cells on the chip were irradiated with alpha particles and stained with a fluorescent probe molecule for DNA damage detection. The CR-39 substrate was etched by means of an alkali solution during cell incubation. The HeLa cells andαtracks were successfully observed by microscopy at once

Research Products

• [Journal Article] Microscopic dose measurement with thin radiophotoluminescence glass plate (2011)
  • Author(s): Maki, D., Nagai, T., Sato, F., Kato, Y., Yamamoto, T., Iida, T.
  • Journal Title: Radiation Measurements Volume: 46 Issue: 12 Pages: 1543-1546
  • DOI: 10.1016/j.radmeas.2011.05.051
  • Peer Reviewed
• [Journal Article] Development of neutron-sensitive glass dosimeter containing isotopically enriched boron (2011)
  • Author(s): Maki, D., Sato, F., Murata, I., Kato, Y., Tanimura, Y., Yamamoto, T., Iida, T.
  • Journal Title: Radiation Measurements Volume: 46 Issue: 12 Pages: 1484-1487
  • DOI: 10.1016/j.radmeas.2011.06.068
  • Peer Reviewed
• [Journal Article] Development of photon counting system with FPGA for precise measurement of radiophotoluminescence (2011)
  • Author(s): Ihara, Y., Ishii, T., Nagai, T., Sato, F., Kato, Y., Yamamoto, T., Iida, T.
  • Journal Title: Radiation Measurements Volume: 46 Issue: 12 Pages: 1574-1577
  • DOI: 10.1016/j.radmeas.2011.05.056
  • Peer Reviewed
• [Journal Article] The recent investigations of radiophotoluminescence and its application (2011)
  • Author(s): Yamamoto, T., Maki, D., Sato, F., Miyamoto, Y., Nanto, H., Iida, T.
  • Journal Title: Radiation Measurements Volume: 46 Issue: 12 Pages: 1554-1559
  • DOI: 10.1016/j.radmeas.2011.04.038
  • Peer Reviewed
• [Journal Article] Development of thermal neutron-sensitive glass dosemeter containing lithium (2011)
  • Author(s): Maki, D., Kobayashi, H., Sato, F., Murata, I., Kato, Y., Tanaka, T., Yamamoto, T., Iida, T.
  • Journal Title: Radiation Protection Dosimetry Volume: 144 Issue: 1-4 Pages: 226-230
  • DOI: 10.1093/rpd/ncq552
  • Peer Reviewed
• [Journal Article] Development of confocal laser microscope system for examination of microscopic characteristics of radiophotoluminescence glass dosemeters (2011)
  • Author(s): Maki, D., Ishii, T., Sato, F., Kato, Y., Yamamoto, T., Iida, T.
  • Journal Title: Radiation Protection Dosimetry Volume: 144 Issue: 1-4 Pages: 222-225
  • DOI: 10.1093/rpd/ncq563
  • Peer Reviewed
• [Journal Article] Development of Cell Chip Based on Track Detector for Examination of Biological Damages by Alpha Particles (2010)
  • Author(s): T. Kuchimaru, F. Sato, S. Tanaka, I. Murata, Y. Kato and T. Iida
  • Journal Title: Journal of Nuclear Science and Technology Volume: 47 Issue: 12 Pages: 1206-1210
  • DOI: 10.1080/18811248.2010.9720987
  • NAID: 10027127029
• [Journal Article] Development of Cell Chip Based on Track Detector for Examination of Biological Damage by Alpha Particles (2010)
  • Author(s): Takahiro Kuchimaru, Fuminobu Sato, Souichi Tanaka, Isao Murata, Yushi Kato, Toshiyuki Iida
  • Journal Title: Journal of Nuclear Science and Technology Volume: 47 Pages: 1206-12010
  • NAID: 10027127029
  • Peer Reviewed
• [Journal Article] New Radiophotoluminescence Glass Dosimeter with Specialized Radiation-Sensitive Surface Layer (2010)
  • Author(s): Daisuke Maki, Hatsumi Kobayashi, Fuminobu Sato, Isao Murata, Yushi Kato, Takayoshi Yamamoto, Toshiyuki Iida
  • Journal Title: Japanese Journal of Applied Physics Volume: 49 Pages: 116401-116405
  • NAID: 40017377308
  • Peer Reviewed
• [Journal Article] Radiophotoluminescent Observation of X-ray Microbeam Track in Silver-Activated Phosphate Glass (2009)
  • Author(s): Y.Aoi, T.Kuchimaru, D.Maki, F.Sato, T.Ikeda, Y.Kato, T.Yamamoto, T.Iida
  • Journal Title: Japanese Journal of Applied Physics 48 Pages: 560011-560014
  • NAID: 40016585529
  • Peer Reviewed
• [Journal Article] Response of a Radio photoluminescence Glass Dosimeter to Low Energy X-rays Necessary for Mammography (2009)
  • Author(s): D.Maki, H.Kobayashi, S.Hisakado, F.Sato, Y.Kato, T.Yamamoto, T.Iida
  • Journal Title: Radiation Safety Management 8 Pages: 8-13
  • NAID: 130001477609
  • Peer Reviewed
• [Journal Article] Development of a Remote-controlled System with the Internet for an Ion Accelerator Aimed at Research and Education on Material Analysis (2009)
  • Author(s): W.Kada, IIshikawa, F.Sato, Y.Kato, T.Iida
  • Journal Title: Journal of Ecotechnology Research 15 Pages: 37-41
  • NAID: 130004956078
  • Peer Reviewed
• [Presentation] Effect of air condition on alpha-radioactivity measurement by air flow pulse ionization chamber (2011)
  • Author(s): Naoki ZUSHI, Yohei IHARA, Keisuke KABASHIMA, Fuminobu SATO, Yushi KATO, Toshiyuki IIDA
  • Organizer: Sixth International Symposium on Radiation Safety and Detection Technology
  • Place of Presentation: Langkawi, Malaysia
• [Presentation] Measurement of radioactive cesium distribution in soil by use of radiophotoluminescence glass dosimeter covered with thin polyethylene film (2011)
  • Author(s): N.ZUSHI, Y.IKEDA, F.SATO, Y.KATO, K.SHIMIZU, N.SATO, T.YAMAMOTO, T.IIDA
  • Organizer: The 7th International Workshop on Individual Monitoring of Ionizing radiation
  • Place of Presentation: Oarai, Ibaraki, Japan
• [Presentation] Development of a bead-type radiophotoluminescence glass dosimeter applicable to various purposes (2011)
  • Author(s): Y.TOYOTA, F.SATO, I.MURATA, Y.KATO, T.IIDA
  • Organizer: The 7th International Workshop on Individual Monitoring of Ionizing radiation
  • Place of Presentation: Oarai, Ibaraki, Japan
• [Presentation] Collaboration research on new glass dosimeters and their applications between Osaka University and Chiyoda Technol Oarai research center (2011)
  • Author(s): T.IIDA, F.SATO, T.YAMAMOTO
  • Organizer: The 7th International Workshop on Individual Monitoring of Ionizing radiation
  • Place of Presentation: Oarai, Ibaraki, Japan(招待講演)
• [Presentation] 単一細胞のX線ビーム照射実験 (2010)
  • Author(s): 田中聡一, 豊田康英, 高橋宏典, 佐藤文信, 清水喜久雄, 加藤裕史, 飯田敏行
  • Organizer: 日本放射線安全管理学会第9回学術大会
  • Place of Presentation: 広島大学
  • Year and Date: 2010-12-02
• [Presentation] 単一細胞のX線ビーム照射実験 (2010)
  • Author(s): 田中聡一, 豊田康英, 高橋宏典, 佐藤文信, 清水喜久雄, 加藤裕史, 飯田敏行
  • Organizer: 日本放射線安全管理学会第9回学術大会広島
  • Place of Presentation: 広島大学東広島キャンパス東広島市
  • Year and Date: 2010-12-01
• [Presentation] X線マイクロビーム照射用神経細胞チップの開発 (2009)
  • Author(s): 田中聡一, 稲川千津, 青位裕輔, 牧大介, 佐藤文信, 加藤裕史, 飯田敏行
  • Organizer: 日本保健物理学会第43回研究発表会
  • Place of Presentation: 大阪
  • Year and Date: 2009-06-03
• [Presentation] Development of Live Cell Chips to Research Effects of X-Ray Radiation to Neuronal Cells (2009)
  • Author(s): C. Inagawa, Y. Aoi, T. Kuchimaru, F. Sato, Y. Kato and T. Iida
  • Organizer: 2nd Asian Congress of Radiation Research
  • Place of Presentation: Seoul, Korea
• [Presentation] Approach to Development of New Glass Dosimeter System (2009)
  • Author(s): T.Iida, F.Sato, H.Kobayashi, W.Kada, S.Hisakado, D.Maki, T.Yamamoto
  • Organizer: Proc.of the 5th International Workshop on Individual Monitoring of Ionizing Radiation
  • Place of Presentation: Ibaraki, Japan
• [Presentation] Development of live Cell Chips to Research Effects of X-ray Radiation to Neuronal Cells (2009)
  • Author(s): C.Inagawa, Y.Aoi, T.Kuchimaru, F.Sato, Y.Kato, T.Iida
  • Organizer: 2^<nd> Asian Congress of Radiation Research
  • Place of Presentation: Seoul, Korean
• [Remarks]
  • URL: http://fusion.eie.eng.osaka-u.ac.jp
• [Remarks]
  • URL: http://fusion.eie.eng.osaka-u.ac.jp