| Project/Area Number |
21651038
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Tohoku Gakuin University |
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Principal Investigator |
ENDO Ginrou 東北学院大学, 工学部, 教授 (80194033)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAUCHI Keisuke 東北学院大学, 工学部, 准教授 (20324014)
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| Co-Investigator(Renkei-kenkyūsha) |
KAN Baihou 東北学院大学, 工学総合研究所, 客員研究員 (20533186)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,010,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2009: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | 環境修復技術 / 環境技術 / 水銀汚染 / 遺伝子水平伝播 / トランスポゾン / 原位置分子育種 / 接合伝達 / 水銀耐性細菌 / 抗生物質耐性最近 / 水銀浄化微生物 / 水銀耐性遺伝子 / 遺伝子多様性 / 遺伝子伝播 / 水銀還元酵素遺伝子merA / 原位置レメディエーション |
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| Research Abstract |
In this study, we analyzed merA genes from the soil sample of a mercury contaminated site. The result showed that there is big diversity between merA genes. Second study was conducted to wide range of merA gene analysis. The results showed that there are many new merA genes in the mercury contaminated soil sample. In the final stage of this research, direct interspecies gene transfer was investigated by using filter-mating method and sandwich-meting method. The mercury resistant and tetracycline resistant bacterial colonies were observed on the culture plates. However, those resistant bacteria were not stable in the liquid culture. From these results, it was concluded that interspecies gene transfer of mercury resistance gene needs special vectors for the horizontal gene transfer.
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