| Project/Area Number |
21651056
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Akita Prefectural University |
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Principal Investigator |
OKANO Keiju 秋田県立大学, 生物資源科学部, 教授 (40147070)
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| Co-Investigator(Kenkyū-buntansha) |
OZAKI Noriaki 秋田県立大学, 生物資源科学部, 助教 (50468120)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,280,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2009: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | フジツボ / 幼生 / 水中接着剤 / タンパク質 / ナノ材料 / AFM / バイオミメティクス / バイオミメティックス |
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| Research Abstract |
Toward the development of biomimetic under-water adhesives inspired by larval cement of the barnacle, Megabalanus rosa, we have obtained the following results.
1) We have developed a method to measure macroscopic forces for marine adhesives using an atomic force microscope. 2) We have developed a microassay to measure the interaction between proteins and clay nanosheets in mock-marine environments using Roche glass capillaries. 3) We have prepared two recombinant cement proteins ; rec-Ccg-57k and rec-Ccg-ASP, both of which, however, did not show positive effects on 1) and 2). 4) We found the strong antimicrobial activity of rec-Ccg-57k against marine GRAM-negative bacteria, Planococcus citreus. 5) In the course of searching unidentified cement components using next generation sequencing, we have found a gene homologous to M. rosa adult cement protein gene mrcp-100k.
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