Development of a novel estimation method of cell functions by creating non-adhesive single cell culture technology
Project/Area Number |
21656218
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Biofunction/Bioprocess
|
Research Institution | Kyushu University |
Principal Investigator |
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2009: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
Keywords | 初代肝細胞 / 非接着浮遊培養 / 細胞機能評価 / 単一細胞培養 / PEG-GRGDS / 細胞骨格 / 薬物代謝 / アルブミン合成 |
Research Abstract |
PEG-GRGDS consisting of polyethylene glycol and arginine-glycine-aspartic acid was prepared. Primary hepatocytes were dispersed by PEG-GRGDS treatment depending on a PEG size. The hepatocytes treated with PEG-GRGDS improved their viability with the cytoskeleton organization, and well expressed albumin production and drug metabolism activities. Therefore, a novel estimation method of cell functions by creating non-adhesive single cell culture technology using PEG-GRGDS was successfully developed.
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Report
(4 results)
Research Products
(13 results)