Development of a novel estimation method of cell functions by creating non-adhesive single cell culture technology

• Project/Area Number: 21656218
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyushu University
• Principal Investigator: IJIMA Hiroyuki 九州大学, 工学研究院, 准教授 (10274515)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2009: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 初代肝細胞 / 非接着浮遊培養 / 細胞機能評価 / 単一細胞培養 / PEG-GRGDS / 細胞骨格 / 薬物代謝 / アルブミン合成

Research Abstract

PEG-GRGDS consisting of polyethylene glycol and arginine-glycine-aspartic acid was prepared. Primary hepatocytes were dispersed by PEG-GRGDS treatment depending on a PEG size. The hepatocytes treated with PEG-GRGDS improved their viability with the cytoskeleton organization, and well expressed albumin production and drug metabolism activities. Therefore, a novel estimation method of cell functions by creating non-adhesive single cell culture technology using PEG-GRGDS was successfully developed.

Research Products

• [Presentation] Non-Adhesive Single Cell Culture for Primary Rat Hepatocytes (2010)
  • Author(s): Hiroyuki Ijima, Jun-ichi Sakai, Szu-Yuan Chou, Philip R Leduc, Yadong Wang
  • Organizer: TERMIS NA 2010(annual meeting of the North Americas Chapter of the Tissue Engineering and Regenerative Medicine International Society in 2010)
  • Place of Presentation: Orlando(U. S. A.)
  • Year and Date: 2010-12-05
• [Presentation] Non-Adhesive Single Cell Culture for Primary Rat Hepatocytes (2010)
  • Author(s): Hiroyuki Ijima, et al.
  • Organizer: TERMIS-NA 2010
  • Place of Presentation: Orlando(U.S.A)
  • Year and Date: 2010-12-05
• [Presentation] RGD-containing molecule for anchoring hepatocytes to cell chip (2010)
  • Author(s): Takayuki Fukuda, Takayuki Takei, Hiroyuki Ijima, Koei Kawakami
  • Organizer: The 23rd International Symposium on Chemical Engineering
  • Place of Presentation: 九州産業大学(福岡市)九州産業大学(福岡市)九州産業大学(福岡市)
  • Year and Date: 2010-12-04
• [Presentation] RGD-containing molecule for anchoring hepatocytes to cell chip (2010)
  • Author(s): Takayuki Fukuda, et al.
  • Organizer: The 23rd International Symposium on Chemical Engineering
  • Place of Presentation: 九州産業大学(福岡市)
  • Year and Date: 2010-12-04
• [Presentation] 初代肝細胞の非接着培養技術の開発 (2010)
  • Author(s): 井嶋博之、堺淳一、武井孝行、川上幸衛
  • Organizer: 第32回日本バイオマテリアル学会大会
  • Place of Presentation: グランドプリンスホテル広島(広島市)
  • Year and Date: 2010-11-29
• [Presentation] 初代肝細胞の非接着単一細胞培養 (2010)
  • Author(s): 井嶋博之、堺淳一、武井孝行、川上幸衛
  • Organizer: 第3回化学工学3支部合同徳島大会
  • Place of Presentation: 徳島大学(徳島市)
  • Year and Date: 2010-10-24
• [Presentation] 初代肝細胞の非接着単一細胞培養技術の開発 (2010)
  • Author(s): 井嶋博之、堺淳一、武井孝行、川上幸衛
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 同志社大学(京都市)
  • Year and Date: 2010-09-08
• [Presentation] 初代肝細胞の非接着単一細胞培養技術の開発 (2010)
  • Author(s): 井嶋博之, 他
  • Organizer: 化学工学会 第42回秋季大会
  • Place of Presentation: 同志社大学(京都市)
  • Year and Date: 2010-09-08
• [Presentation] Non-adhesive single-cell culture method for primary rat hepatocyte (2010)
  • Author(s): Hiroyuyki Ijima, Jun-ichi Sakai, Takayuki Takei, Koei Kawakami
  • Organizer: The 23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology(JAACT2010)
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2010-09-03
• [Presentation] Non-adhesive single-cell culture method for primary rat hepatocyte (2010)
  • Author(s): Hiroyuyki Ijima, et al.
  • Organizer: JAACT 2010
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2010-09-03
• [Presentation] RGD配列と基板への固定化部位を有するタンパク質の開発とその性能評価 (2010)
  • Author(s): 福田貴之,久保孝文,堺淳一,武井孝行,境慎司,井嶋博之,川上幸衛
  • Organizer: 第12回化学工学会学生発表会(福岡大会)
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2010-03-06
• [Presentation] RGD配列と基板への固定化部位を有するタンパク質の開発とその性能評価 (2010)
  • Author(s): 福田貴之, 他
  • Organizer: 第12回 化学工学会 学生発表会(福岡大会)
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2010-03-06
• [Presentation] 壁付着性動物細胞のシングルセル培養のための基礎的検討 (2009)
  • Author(s): 堺淳一,武井孝行,境慎司,井嶋博之,川上幸衛
  • Organizer: 第2回化学工学3支部合同北九州大会
  • Place of Presentation: 西日本総合展示場(北九州市)
  • Year and Date: 2009-10-30