| Budget Amount *help |
¥3,410,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2009: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Research Abstract |
In order to elucidate the mechanism of pressure adaptation of proteins, we have developed structural studies of proteins by a high-pressure protein crystallography method using a diamond-anvil cell. IPMDH from the deep-sea bacterium Shewanella benthica DB21MT-2 is more tolerant towards HP stress than its counterpart from the land bacterium S. oneidensis MR-1, even though these two enzymes share 85% amino-acid identity. Crystal structures of IPMDH have been determined at about 2 A resolution under pressures ranging from 0. 1 to 650 MPa. Penetration of water molecules into IPMDH is observed at high-pressure, and relationship of the structure and pressure resistance has been elucidated.
|
