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Metagenomic analysis of microbial population in intercellular fluid of rice and its application for disease control

Research Project

Project/Area Number 21658015
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Plant pathology
Research InstitutionTohoku University

Principal Investigator

TAKAHASHI Hideki  東北大学, 大学院・農学研究科, 教授 (20197164)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,310,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2009: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords植物 / 遺伝子 / メタゲノム / 内生菌 / 植物病理学
Research Abstract

Microorganisms inhabiting in intercellular spaces of plants may stimulate plant defense systems or promote plant growth, whereas they are likely to benefit from the exudates of plant cells. In this study, we analyzed the structure of overall community of endophytic microorganisms in plants and their disease suppressive activity. Intercellular fluid (IF)was collected from leaf blades and sheaths of rice.(1)Denaturing gradient gel electrophoresis (DGGE)pattern and nucleotide sequence analyses of 16S and 18S_rDNA fragments amplified from IF DNA seemed to be available to understand global microbial ecology in plants.(2)Pseudomonas sp., bcillus sp., Curtobacterium sp. and Acinetobacter sp. were preferentially isolated as endophytic bacteria from rice IF cultivated by organic farming system.(3)Some of those endophytic bacteria exhibited potential activity to suppress rice blast and rice bacterial rot seedling diseases.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (16 results)

All 2012 2011 2010 2009

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (7 results) Book (5 results)

  • [Journal Article] Biocontrol of black scurf on potato by seed tuber treatment with Pythium oligandrum2012

    • Author(s)
      Ikeda, S., Shimizu, A., Shimizu, M., Takahashi, H. and Takenaka, S.
    • Journal Title

      Biological Control

      Volume: 60 Pages: 297-304

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Implications of oligomeric forms of POD-1 and POD-2 proteins isolated from cell walls of the biocontrol agent Pythium oligandrum in relation to their ability to induce defense reactions in tomato2011

    • Author(s)
      Takenaka, S., Yamaguchi, K., Masunaka, A., Hase, S., Inoue, T. and Takahashi, H.
    • Journal Title

      Journal of Plant Physiology

      Volume: 168 Pages: 1972-1979

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial community profiles in intercellular fluid of rice2011

    • Author(s)
      Takahashi, H., et al.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 77 Pages: 121-131

    • NAID

      10029720895

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial community profiles in intercellular fluid of rice2010

    • Author(s)
      Takahashi, H., Sekiguchi, H., Ito, T., Sasahara, M., Hatanaka, N. Ohba, A., Hase, S., Ando, S. and Takenaka, S.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 77 Pages: 121-131

    • NAID

      10029720895

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] 有機栽培育苗土によるイネもみ枯細菌病抑制効果の解析2011

    • Author(s)
      安藤杉尋・對馬誠也・吉田重信・長谷川浩・小林隆・伊藤豊彰・高橋英樹
    • Organizer
      平成23年度日本植物病理学会東北部会
    • Place of Presentation
      青森市
    • Year and Date
      2011-11-03
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 有機栽培イネ細胞間隙に由来する微生物の解析と病害防除2011

    • Author(s)
      安藤杉尋・高橋英樹
    • Organizer
      平成23年度日本土壌微生物学会大会
    • Place of Presentation
      宮城県鳴子町
    • Year and Date
      2011-10-23
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] イネ内生菌のメタゲノム解析と病害防除への展望2010

    • Author(s)
      高橋英樹
    • Organizer
      第17回山形植物防疫懇談会
    • Place of Presentation
      鶴岡市
    • Year and Date
      2010-12-04
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 有機栽培イネ地上部より単離された内生細菌のイネいもち病抵抗性への影響2010

    • Author(s)
      安藤杉尋・伊藤豊彰・長谷川浩・竹中重仁・長谷修・生井恒雄・高橋英樹
    • Organizer
      平成22年度日本植物病理学会東北部会
    • Place of Presentation
      秋田市
    • Year and Date
      2010-10-05
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 有機栽培イネからの内生細菌の分離と16SrDNA断片塩基配列による解析2010

    • Author(s)
      高橋英樹・安藤杉尋・伊藤豊彰・長谷川浩・竹中重仁
    • Organizer
      平成22年度日本植物病理学会東北部会
    • Place of Presentation
      秋田市
    • Year and Date
      2010-10-05
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 品種, 地域, 生育時期, 栽培条件が異なるイネ地上部の内生菌集団から増幅された16S rDNA-ITS断片のPCR-DGGE法による比較解析2010

    • Author(s)
      高橋英樹・関口博之・安藤杉尋・長谷川浩・竹中重仁
    • Organizer
      平成21年度日本植物病理学会東北部会
    • Place of Presentation
      仙台市
    • Year and Date
      2010-09-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 品種,地域,生育時期,栽培条件が異なるイネ地上部の内生菌集団から増幅された16S rDNA-ITS断片のPCR-DGGE法による比較解析2009

    • Author(s)
      高橋英樹, ら
    • Organizer
      平成21年度日本植物病理学会東北部会
    • Place of Presentation
      宮城県立大学(仙台市)
    • Year and Date
      2009-09-29
    • Related Report
      2009 Annual Research Report
  • [Book] 日本発の抵抗性誘導微生物Pythium oligandrum研究の現状2012

    • Author(s)
      竹中重仁・高橋英樹
    • Publisher
      日本植物防疫協会(印刷中)
    • Related Report
      2011 Final Research Report
  • [Book] 土と微生物(Soil Microorganisms), 有機栽培イネ細胞間隙に由来する微生物の解析と病害防除(Analysis of endophytes from intercellular fluid of organically-grown rice plants and its effect on disease tolerance)2011

    • Author(s)
      安藤杉尋・高橋英樹
    • Publisher
      日本土壌微生物学会
    • Related Report
      2011 Final Research Report
  • [Book] 土と微生物「有機栽培イネ細胞間隙に由来する微生物の解析と病害防除」2011

    • Author(s)
      安藤杉尋・高橋英樹
    • Total Pages
      3
    • Publisher
      日本土壌微生物学会
    • Related Report
      2011 Annual Research Report
  • [Book] 「遺伝子発現からみた誘導抵抗の全体像」微生物と植物の相互作用. 病害と生物防除2009

    • Author(s)
      高橋英樹・竹中重仁
    • Publisher
      ソフトサイエンス社
    • Related Report
      2011 Final Research Report
  • [Book] 「遺伝子発現からみた誘導抵抗の全体像」微生物と植物の相互作用-病害と生物防除-(百町満朗・対馬誠也編)第2章2-12009

    • Author(s)
      高橋英樹・竹中重仁
    • Publisher
      ソフトサイエンス社
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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