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Does apomixis occur to cause genomic duplication and clonal reproduction in sturgeons?

Research Project

Project/Area Number 21658067
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionHokkaido University

Principal Investigator

ARAI Katsutoshi  北海道大学, 大学院・水産科学研究院, 教授 (00137902)

Co-Investigator(Kenkyū-buntansha) ADACHI Shinji  北海道大学, 大学院・水産科学研究院, 教授 (40231930)
YAMAHA Eturo  北海道大学, 北方生物圏フィールド科学センター, 教授 (60191376)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsゲノムサイズ / 倍数体 / 生殖細胞 / 染色体 / FISH / 雑種 / 希少種 / 凍結保存 / フローサイトメトリー / 四倍体 / 三倍体 / fluorescens in situ hybridization / 精子 / DNA量 / C-値 / 交雑種 / 人工授精
Research Abstract

Possible involvement of "apomixis", which causes clonal reproduction and polyploidy in plants, was investigated in sturgeon species. Genome size estimation by DNA content flow cytometry and molecular cytogenetic analyses including FISH clearly showed elevation and reduction of ploidy status among different species as well as occurrence of genetic diploid and tetraploid in artificially propagated con-specific and hybrid progeny. The results suggest ploidy variation should happen in present sturgeon species.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (19 results)

All 2012 2011 2010 2009

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (18 results)

  • [Journal Article] Genome size variation estimated by flow cytometry in Acipenser mikadoi, Huso dauricus and other species of Acipenseriformes reared in Japna2011

    • Author(s)
      He Zhou, Takafumi Fujimoto, Shinji Adachi, Etsuro Yamaha, Katsutoshi Arai
    • Journal Title

      Journal of Applied Ichthyology

      Volume: 27巻 Issue: 2 Pages: 484-491

    • DOI

      10.1111/j.1439-0426.2010.01648.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Presentation] 卵成熟能および排卵能を指標としたチョウザメ類採卵時期の推定2012

    • Author(s)
      石原学・徳井文平・安部智貴・山羽悦郎・木村志津雄・井尻成保・足立伸次
    • Organizer
      平成24年日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 卵成熟能および排卵能を指標としたチョウザメ類採卵適期の推定2012

    • Author(s)
      石原学・徳井文平・安部智貴・山羽悦郎・木村志津雄・井尻成保・足立伸次
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] チョウザメ始原生殖細胞のFITC-biotin dextransによる可視化2011

    • Author(s)
      斎藤大樹・後藤理恵・Psenicka Martin・川上優・足立伸次・荒井克俊・山羽悦郎
    • Organizer
      平成23年度日本水産学会秋季大会
    • Place of Presentation
      長崎大学(長崎市)
    • Year and Date
      2011-09-29
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Isolation of sturgeon spermatogonia as material for biotechnological application2011

    • Author(s)
      Maritin Psenicka, Taiju Saito, Shinji Adachi, Katsutoshi Arai, Etsuro Yamaha
    • Organizer
      3^<rd> International Workshop on the Biology of Fish Gametes
    • Place of Presentation
      ブダペスト(ハンガリー)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Final Research Report
  • [Presentation] Origin and migration of primordial germ cells in sturgeon2011

    • Author(s)
      Taiju Saito, Rie Goto, Martin Psenicka, Yutaka Kawakami, Shinji Adachi, Katsutoshi Arai, Etsuro Yamaha
    • Organizer
      3^<rd> International Workshop on the Biology of Fish Gametes
    • Place of Presentation
      ブダペスト(ハンガリー)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Final Research Report
  • [Presentation] Origin and migration of primordial germ cells in sturgeon2011

    • Author(s)
      Saito T, Goto R, Psenicka M, Kawakami Y, Adachi S, Arai k, Yamaha E
    • Organizer
      3rd International Workshop on the Biology of Fish Gametes
    • Place of Presentation
      Danubius Hotel Helia(ハンガリー)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Isolation of sturgeon spermatogonia as material for biotechnological application2011

    • Author(s)
      Psenicka M, Saito T, Adachi S, Arai K, Yamaha E
    • Organizer
      3rd International Workshop on the Biology of Fish Gametes
    • Place of Presentation
      Danubius Hotel Helia(ハンガリー)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] チョウザメの卵成熟能および排卵能の経時的変化2011

    • Author(s)
      石原学・安部智貴・山羽悦郎・木村志津雄・井尻成保・足立伸次
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2011-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] チョウザメの始原生殖細胞の起源と移動2011

    • Author(s)
      斎藤大樹・後藤理恵・Psenicka Martin・川上優・足立伸次・荒井克俊・山羽悦郎
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2011-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] 二倍体および三倍体ミカドチョウザメAcipenser mikadoiの核型とFISHによるrDNAの検出2011

    • Author(s)
      周賀・藤本貴史・足立伸次・山羽悦郎・荒井克俊
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2011-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Ultrastructure of Acipenser mikadoi spermatozoa(ミカドチョウザメ精子の微細構造)2010

    • Author(s)
      Psenicka Martin・足立伸次・山羽悦郎・荒井克俊
    • Organizer
      平成22年度日本水産学会秋季大会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2010-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] ミカドチョウザメ精子の微細構造2010

    • Author(s)
      Psenicka M、足立伸次, ほか2名
    • Organizer
      平成22年度日本水産学会秋季大会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] チョウザメの生体外卵成熟、排卵および発生誘導2010

    • Author(s)
      安部智貴・石原学・山下勝正・申東煥・井尻成保・足立伸次
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日本大学(藤沢市)
    • Year and Date
      2010-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] チョウザメの生体外卵成熟、排卵および発生誘導2010

    • Author(s)
      安部智貴・石原学・山下勝正・申東煥・井尻成保・足立伸次
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      藤沢市、日本大学
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Genome size variation estimated by nuclear DNA content flow cytometry in ten sturgeon species and several interspecific hybrids reared in Japan2009

    • Author(s)
      He Zhou・Takafumi Fujimoto, Shinji Adachi, Etsuro Yamaha, Katsutoshi Arai
    • Organizer
      6^<th> International Symposium on Sturgeons
    • Place of Presentation
      武漢市(中国)
    • Year and Date
      2009-10-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Genome size variation estimated by nuclear DNA content flow cytometry in ten sturgeon species and several interspecific hybrids reared in Japan2009

    • Author(s)
      Zhou H, Fujimoto T, Adachi S, Yamaha E, Arai K
    • Organizer
      6^<th> International Symposium on Sturgeon
    • Place of Presentation
      中国武漢市
    • Year and Date
      2009-10-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Diploid and polyploidy karyotypes observed in the progeny of artificially propagated Mikado sturgeon Acipenser medirostris mikadoi2009

    • Author(s)
      He Zhou・Takafumi Fujimoto, Shinji Adachi, Etsuro Yamaha, Katsutoshi Arai
    • Organizer
      6^<th> International Symposium on Sturgeons
    • Place of Presentation
      武漢市(中国)
    • Related Report
      2011 Final Research Report
  • [Presentation] Diploid and polyploidy karyotypes observed in the progeny of artificially propagated Mikado sturgeon Acipenser medirostris maikadoi2009

    • Author(s)
      Zhou H, Fujimoto T, Adachi S, Yamaha E, Arai K
    • Organizer
      6^<th> International Symposium on Sturgeon
    • Place of Presentation
      中国武漢市
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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