Construction of cDNA library for the elucidation of mechanism occurred in non-toxic mutant of dinoflagellate

• Project/Area Number: 21658075
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Fisheries chemistry
• Research Institution: Tohoku University
• Principal Investigator: CHO Yuko 東北大学, 大学院・農学研究科, 助教 (60323086)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,410,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2009: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 水産学 / プランクトン / 麻痺性貝毒 / 無毒変異 / cDNAライブラリー

Research Abstract

Single-cell analysis of paralytic shellfish toxins by HPLC with post column fluorescent derivatization revealed the large variety of toxin productivity among cells from the clonal strain of A. tamarense, a PST-producing dinoflagellate. The different growth rate and toxin contents were observed among the cells in the identical culture well at the early stage of incubation after re-isolation. The fundamental data for the construction of the single-cell library was obtained

Research Products

• [Journal Article] Effects of mitomycin C and colchicine on toxin production and cell cycle regulation in the dinoflagellate (2011)
  • Author(s): Yuko Cho, Motoo Ogawa, Mayumi Hirota, and Yasukatsu Oshima, Effects of mitomycin C and colchicine on toxin
  • Journal Title: Alexandrium tamarense, Harmful Algae Volume: 10 Pages: 235-244
  • Peer Reviewed
• [Journal Article] Effects of mitomycin C and colchicine on toxin production and cell cycle regulation in the dinoflagellate, Alexandrium tamarense (2011)
  • Author(s): Yuko Cho, Motoo Ogawa, Mayumi Hirota, Yasukatsu Oshima
  • Journal Title: Harmful Algae Volume: 10 Pages: 235-244
  • Peer Reviewed
• [Presentation] Yuko Cho, Ryoko Ozeki and Yasukatsu Oshima, Variety of toxin contents in a clonal culture of Alexandrium tamarense determined by single-cell analysis using HPLC with post column fluorescent derivatization for paralytic shellfish toxins (2010)
  • Author(s): uko Cho, Ryoko Ozeki and Yasukatsu Oshima
  • Organizer: 環太平洋国際化学会議
  • Place of Presentation: ホノルル、アメリカ合衆国
• [Presentation] Variety of toxin contents in a clonal culture of Alexandrium tamarense determined by single-cell analysis using HPLC with post column fluorescent derivatization for paralytic shellfish toxins (2010)
  • Author(s): Yuko Cho, Ryoko Ozeki, Yasukatsu Oshima
  • Organizer: 環太平洋国際化学会議
  • Place of Presentation: ホノルル、アメリカ合衆国
• [Presentation] 渦鞭毛藻Alexandrium tamarense麻痺性貝毒量の一細胞分析 (2009)
  • Author(s): 長由扶子、尾関諒子、大島泰克
  • Organizer: 第6回東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-06-16
• [Presentation] Single-cell analysis of paralytic shellfish toxins in Alexandrium tamarense by HPLC with post column fluorescent derivatization (2009)
  • Author(s): Yuko Cho, Ryoko Ozeki and Yasukatsu Oshima
  • Organizer: ゴードン会議
  • Place of Presentation: ニューロンドン、アメリカ合衆国