Creation of rescue system for potential oocytes by apoptosis inhibitory factor gene transfer and xenotransplantation techniques
Project/Area Number |
21658092
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Applied animal science
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Research Institution | The University of Tokyo |
Principal Investigator |
MANABE Noboru 東京大学, 大学院・農学生命科学研究科, 教授 (80243070)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2009: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
Keywords | 卵胞閉鎖 / 卵母細胞 / アポトーシス / 細胞死阻害因子 / ブタ / アポトーシス阻害因子 / 卵胞 / 顆粒層細胞 / 卵巣 / 卵巣内潜在的卵母細胞 / 細胞死リガンド・受容体 / カスパーゼ |
Research Abstract |
In mammals, hundreds of thousands of oocytes are contained in adult ovaries, and small part of oocytes restart meiosis in each estrous cycle. More than 99% of follicles are regenerated as atresia, however, molecular control mechanism to select ovulating oocytes which is an important strategy to get excellent offspring has not been revealed. The present study performed to elucidate the molecular control mechanism that regulates a selective killing of ovarian follicles in porcine ovaries, in other words, to examine the apoptosis regulating molecular mechanism in granulosa cells. I analyzed the transmission system of apoptotic signal in granulosa cells. I revealed that granulosa cell was a type II apoptosis cell in which apoptotic signal transduced via mitochondria. I found that a novel soluble receptor, an outside factor of cell membrane, inhibited cell death in garnulosa cells. Moreover, I demonstrated two inhibitors, cFLIP and XIAP, which interrupted apoptotic signaling within the cells. For application of these apoptosis inhibiting factors, I created the rescue system for potential oocytes in frozen small ovarian tissues by apoptosis inhibitory factor gene transfer and xenotransplantation techniques to raise the follicle survival rate.
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Report
(4 results)
Research Products
(133 results)
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[Journal Article] Follicular turnover and hormonal association in postpartum goats during early and late lactation2012
Author(s)
Mohamed M M Kandiel, Gen Watanabe, Mahmoud E A, Abou-El-Roos, Alaa E Abdel-Ghaffar, Gamal A Sosa, Abd El-Salam I El-Azab, Kentaro Nagaoka, Jun-You Li, Noboru Manabe, Kazuyoshi Taya
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Journal Title
The Journal of Reproduction and Development
Volume: 58
Pages: 61-68
NAID
Related Report
Peer Reviewed
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[Journal Article] 福島第一原子力発電所事故後の茨城県産牧草を給与した牛の乳における放射性核種物質濃度2012
Author(s)
橋本健, 田野井慶太朗, 桜井健太, 飯本武志, 野川憲夫, 桧垣正吾, 小坂尚樹, 高橋友継, 榎本百合子, 小野山一郎, 李俊佑, 眞鍋昇, 中西友子
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Journal Title
Radioisotopes
Volume: 60
Pages: 335-338
Related Report
Peer Reviewed
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