Regeneration of rare birds through germline chimera.

• Project/Area Number: 21658093
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied animal science
• Research Institution: Shinshu University
• Principal Investigator: KAGAMI Hiroshi 信州大学, 農学部, 教授 (80308303)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2009: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: 希少鳥類 / 生殖系列キメラ / 代理親 / 始原生殖細胞 / 生殖キメラ / 鳥類保全 / 発生解析 / 生殖細胞キメラ / 個体増殖 / 遺伝資源保存

Research Abstract

PGCs of Gifujidori fowl were collected from the blood of early embryos for transfer to recipients. The embryos were cultured until hatching fertility tests indicated that they had normal reproductive abilities. White Leghorn were used as recipients for chimera production following blood removal. The PGCs were microinjected into the recipients obtaining offspring from Gifujidori. In conclusion, PGCs from the embryos was combined with the conservation of live animals.

Research Products

• [Journal Article] Viability and Functionality of Primordial Germ Cells after Freeze-thaw in Chickens (2011)
  • Author(s): Nakamura, Y., Usui, F., Miyahara, D., Mori, T., Watanabe, H., Ono, T., Takeda, K., Nirasawa, K., Kagami, H. and Tagami, T
  • Journal Title: Journal of Poultry Science Volume: 48 Pages: 57-63
  • NAID: 10027817694
  • Peer Reviewed
• [Journal Article] Viability and Functionality of Primordial Germ Cells after Freeze-thaw in Chickens. (2011)
  • Author(s): Hiroshi Kagami
  • Journal Title: Journal of Poultry Science Volume: 48 Pages: 57-63
  • NAID: 10027817694
  • Peer Reviewed
• [Journal Article] Efficient System for Preservation and Regeneration of Genetic Resources in Chicken : Concurrent Store of Primordial Germ Cells and Live Animals from early Embryos of A Rare Indigenous Fowl(Gifujidoro) (2010)
  • Author(s): Nakamura, Y., Usui, F., Miyahara, D., Mori, F., Ono, T., Takeda, K., Nirasawa, K., Kagami, H and Tagami, T
  • Journal Title: Reproduction, Fertility and Development Volume: 22 Pages: 1237-1344
  • Peer Reviewed
• [Journal Article] Germline Replacement by Transfer of Primordial Germ Cells into Partially Sterilized Embryos in the Chicken (2010)
  • Author(s): Nakamura, Y., Usui, F., Ono, T., Takeda, K., Nirasawa, K., Kagami, H and Tagami, T
  • Journal Title: Biology of Reproduction Volume: 83 Pages: 130-137
  • Peer Reviewed
• [Journal Article] A Novel Concentration System of Chicken Stem Cells by Bone Marrow Side Population Cells (2010)
  • Author(s): Usui, F., Nakamura, Y., Yamamoto, Y., Tatsumi, K., Tominari, K., Ono, T. and Kagami, H
  • Journal Title: Journal of Poultry Science Volume: 47 Pages: 53-56
  • NAID: 10026253103
  • Peer Reviewed
• [Journal Article] Efficient System for Preservation and Regeneration of Genetic Resources in Chicken: Concurrent Store of Primordial Germ Cells and Live Animals from early Embryos of A Rare Indigenous Fowl(Gifujidoro). (2010)
  • Author(s): Hiroshi Kagami
  • Journal Title: Reproduction, Fertility and Development Volume: 22 Pages: 1237-1344
  • Peer Reviewed
• [Journal Article] Effects of Busulfan sustained-release emulsion on depletion and repopulation of primordial germ cells in early chicken embryos. (2009)
  • Author(s): Hiroshi Kagami
  • Journal Title: The Journal of Poultry Science 46 Pages: 127-135
  • NAID: 10024799424
  • Peer Reviewed
• [Presentation] Isolation of Adipose-tissue Derived Stem Cells and the Application for Blood Vessel Regeneration in the Chicken (2012)
  • Author(s): Watanabe, H., Usui, F., Miyahara, D., Mori, T., Ono, T., and Kagami, H
  • Organizer: International Symposium on Animal Biotechnology
  • Place of Presentation: Minamiminowa
  • Year and Date: 2012-01-31
• [Presentation] Long Term Culture and Characterization of Chicken Primordial Germ Cells (2012)
  • Author(s): Miyahara, D., Nakamura, Y., Mori, T., Ono, T., Nirasawa, K., IOishi, I., Tagami, T., and Kagami, H.
  • Organizer: International Symposium on Animal Biotechnology
  • Place of Presentation: Minamiminowa
  • Year and Date: 2012-01-31
• [Presentation] Avian Stem Cells for Regeneration of Muscular Dystrophy Chickens by Means of Germline Chimeras (2012)
  • Author(s): Fujiwara, A., Mizutani, M., Nunooya, T., Hiramatsu, K., Ono, T., and Kagami, H
  • Organizer: International Symposium on Animal Biotechnology
  • Place of Presentation: Minamiminowa
  • Year and Date: 2012-01-31
• [Presentation] Long tem culture and characterization of chicken primordial germ cells (2012)
  • Author(s): Daichi Miyahars, et al, Hiroshi Kagami
  • Organizer: International Symposium on Animal Biotechnology
  • Place of Presentation: 長野県、信州大学
  • Year and Date: 2012-01-31
• [Presentation] J. Avian Stem Cells and the Application for Biotechnology (2010)
  • Author(s): Kagami, H., Usui, F., Nakamura, Y., Kashiwagi, M., Yamamoto, Y., Ono, T., Tagami, T., Nirasawa, K., Matsubara, Y., and Petitte
  • Organizer: Japanese Society of Developmental Biologist
  • Place of Presentation: Kyoto
  • Year and Date: 2010-06-23
• [Presentation] ニワトリ幹細胞・始原生殖細胞の遺伝的特徴解析と分化制御の試み (2010)
  • Author(s): 宮原大地、今井一樹、森貴史、渡邉晴陽、坂田絢子、坪井友子、都築明香里、中村隼明、臼井文武、山本耕裕、田上貴寛、韮澤圭二郎、松原悠子、川口耕一郎、藤井博、小野珠乙、鏡味裕
  • Organizer: 日本分子生物学会第10回春季シンポジウム
  • Place of Presentation: 松島
  • Year and Date: 2010-06-07
• [Presentation] ニワトリ始原生殖細胞の利用:移動様式の解明と凍結保存・移植・除去技術の確立 (2010)
  • Author(s): 中村隼明、宮原大地、森貴史、今井一樹、渡邉晴陽、小野珠乙、武田久美子、韮澤圭二郎、鏡味裕、田上貴寛
  • Organizer: 日本分子生物学会第10回春季シンポジウム
  • Place of Presentation: 松島
  • Year and Date: 2010-06-07
• [Presentation] 始原生殖細胞の新規採取法の開発と生殖細胞キメラ作出への活用 (2010)
  • Author(s): 鏡味裕、柏木まや、大屋智子、臼井文武、中村隼明、山本耕裕、小野珠乙
  • Organizer: 日本家禽学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-30
• [Presentation] ニワトリ幹細胞及び始原生殖細胞の発生に伴う遺伝子発現の解析と分化制御への活用の試み (2009)
  • Author(s): 鏡味裕、臼井文武、中村隼明、山本耕裕、大友朝子、柏木まや、田上貴寛、韮澤圭二郎、松原悠子、小野珠乙
  • Organizer: 日本畜産学会大会
  • Place of Presentation: 那覇
  • Year and Date: 2009-09-28
• [Book] 鳥類の受精、発生、胚操作.新動物生殖学(佐藤英明編著) (2011)
  • Author(s): 鏡味裕
  • Publisher: 朝倉書店
• [Book] 新動物生殖学(佐藤英明編著) (2011)
  • Author(s): 鏡味裕
  • Total Pages: 203
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://karamatsu.shinshu-u.ac.jp/lab/ono_kagami/hasseiken.htm