Morphological analyses on the biogenesis and extension mechanisms of autophagic isolation membrane

• Project/Area Number: 21659045
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Fukushima Medical University
• Principal Investigator: WAGURI Satoshi 福島県立医科大学, 医学部, 教授 (30244908)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,460,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2009: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: 細胞微細形態学 / オートファジー / Atg遺伝子 / 線維芽細胞 / Atgタンパク質 / オートファゴソーム / 隔離膜 / 電子顕微鏡 / DFCP1 / オメガソーム / 電子線トモグラフィー法

Research Abstract

Fine structures of autophagic isolation membrane induced by amino acid deprivation in mouse embryonic fibroblasts were investigated. By using newly developed fixation protocol, we found a cluster of narrow tubular structures between the endoplasmic reticulum and isolation membranes. Moreover, they corresponded to"omegasome"that has been identified only at light microscopic levels. These findings could promote research of membrane dynamics and associated molecular mechanisms in the biogenesis of autophagic isolation membrane.

Research Products

• [Journal Article] Persistent activation of Nrf2 through p62 in hepatocellular carcinoma cells (2011)
  • Author(s): Inami Y, Waguri S, Sakamoto A, Kouno T, Nakada K, Hino O, Watanabe S, Andoh J, Iwadate M, Yamamoto M, Myung-Shik Lee MS, Tanaka K, Komatsu M.
  • Journal Title: J Cell Biol Volume: 193 Pages: 275-284
  • Peer Reviewed
• [Journal Article] Autophagy-deficient mice develop multiple liver tumors (2011)
  • Author(s): Takamura T, Komatsu M, Hara H, Sakamoto A, Kishi1 C, Waguri S, Eishi Y, Hino O, Tanaka K, Mizushima N.
  • Journal Title: Genes Dev Volume: 25 Pages: 795-800
  • Peer Reviewed
• [Journal Article] OATL1 a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation (2011)
  • Author(s): Itoh, T, Kanno, E, Uemura, T, Waguri, S, Fukuda, M.
  • Journal Title: J Cell Biol Volume: 192 Pages: 839-853
  • Peer Reviewed
• [Journal Article] Autophagy-deficient mice develop multiple liver tumors (2011)
  • Author(s): Takamura, A., Komatsu, M., Hara, T., Sakamoto, A., Kishi, C., Waguri, S., Eishi, Y., Hino O., Tanaka, K., Mizushima, N
  • Journal Title: Genes Dev. Volume: 25 Issue: 8 Pages: 795-800
  • DOI: 10.1101/gad.2016211
  • Peer Reviewed
• [Journal Article] Persistent activation of Nrf2 through p62 in hepatocellular carcinoma cells (2011)
  • Author(s): Inami Y, Waguri S, Sakamoto A, Kouno T, Nakada K, Hino O, Watanabe S, Ando J, Iwadate M, Yamamoto M, Lee MS, Tanaka K, Komatsu M
  • Journal Title: J Cell Biol Volume: 193 Issue: 2 Pages: 275-284
  • DOI: 10.1083/jcb.201102031
  • Peer Reviewed
• [Journal Article] OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation. (2011)
  • Author(s): 伊藤敬
  • Journal Title: Journal of Cell Biology Volume: 192 Pages: 839-853
  • Peer Reviewed
• [Journal Article] Mechanism of statin-induced contractile dysfunction in rat cultured skeletal myofibers (2010)
  • Author(s): Tanaka S, Sakamoto K, Yamamoto M, Mizuno A, Ono T, Waguri S, Kimura J.
  • Journal Title: J Pharmacol Sci Volume: 114 Pages: 454-463
  • Peer Reviewed
• [Journal Article] The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1 (2010)
  • Author(s): Komatsu M, Kurokawa H, Waguri S, Taguchi K, Kobayashi A, Ichimura Y, Sou YS, Ueno I, Sakamoto A, Tong KI, Kim M, Nishito Y, Iemura S, Natsume T, Ueno T, Kominami E, Motohashi H, Tanaka K, Yamamoto M.
  • Journal Title: Nat Cell Biol Volume: 12 Pages: 213-223
  • Peer Reviewed
• [Journal Article] Measurement of autophagy in cells and tissues. (2010)
  • Author(s): 谷田以誠
  • Journal Title: Methods in Molecular Biology Volume: 648 Pages: 193-214
• [Journal Article] Mechanism of statin-induced contractile dysfunction in rat cultured skeletal myofibers. (2010)
  • Author(s): 田中祥子
  • Journal Title: Journal of Pharmacological Sciences Volume: 114 Pages: 454-463
  • Peer Reviewed
• [Journal Article] The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keapl (2010)
  • Author(s): Komatsu M
  • Journal Title: Nature Cell Biology 12 Pages: 213-223
  • Peer Reviewed
• [Presentation] オートファジーに関与するRab-GAPの探索 (2011)
  • Author(s): 伊藤敬、菅野栄子、植村武文、和栗聡、福田光則
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-03-28
• [Presentation] Atg欠損マウス線維芽細胞株におけるオートファジー関連構造の形態学的相互関係 (2011)
  • Author(s): 和栗聡
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] オートファジーに関与するRab-GAPの探索 (2010)
  • Author(s): 伊藤敬
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Atg3欠損マウス線維芽細胞株におけるオメガソームマーカーDFCP1の局在解析 (2010)
  • Author(s): 亀高愛、山田茜、澤田直樹、山本雅哉、曽友深、小松雅明、和栗聡
  • Organizer: 第56回東北・北海道連合支部学術集会
  • Place of Presentation: 旭川
  • Year and Date: 2010-09-25
• [Presentation] Atg3欠損マウス線維芽細胞株におけるオメガソームマーカーDFCP1の局在解析 (2010)
  • Author(s): 和栗聡
  • Organizer: 日本解剖学会第56回東北・北海道連合支部学術集会
  • Place of Presentation: 旭川
  • Year and Date: 2010-09-25
• [Presentation] Atg3欠損マウス線維芽細胞株で見られたオートファゴソーム様構造の形態学的解析 (2010)
  • Author(s): 山田茜、亀高愛、山本雅哉、曽友深、小松雅明、和栗聡
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-30
• [Presentation] ラット皮膚創傷治癒過程におけるMAP1LC3の免疫組織学的動態解析 (2010)
  • Author(s): 浅井笑子、山本雅哉、上田和毅、和栗聡
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-30
• [Presentation] Atg3欠損マウス線維芽細胞で見られたオートファゴソーム様構造の形態学的解析 (2010)
  • Author(s): 山田茜
  • Organizer: 第115回日本解剖学会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-30
• [Presentation] ラット皮膚創傷治癒過程におけるMAP1LC3の免疫組織学的動態解析 (2010)
  • Author(s): 浅井笑子
  • Organizer: 第115回日本解剖学会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-30
• [Book] タンパク質分解系による生体制御:オートファジーの評価方法 (2011)
  • Author(s): 谷田以誠、上野隆、和栗聡
• [Book] (書名)「タンパク質分解系による生体制御」(タイトル)「オートファジーの評価方法」 (2011)
  • Author(s): 谷田以誠、上野直人、和栗聡
  • Publisher: 羊土社
• [Book] Measurement of autophagy in cells and tissues Methods Mol Biol (2010)
  • Author(s): Tanida I, Waguri S.
• [Remarks]
  • URL: http://www.fmu.ac.jp/home/anatomy2/anatomy2_index.html
• [Remarks]
  • URL: http://www.fmu.ac.jp/home/anatomy2/anatomy2_index.html
• [Remarks]
  • URL: http://www.fmu.ac.jp/home/anatomy2/anatomy2_index.html
• [Remarks]