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Detection of VSEL cells in umbilical cord blood and development of co-culture system with umbilical cord-derived mesehchymal stem cells

Research Project

Project/Area Number 21659069
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

NAGAMURA Tokiko (INOUE Tokiko)  東京大学, 医科学研究所, 講師 (70240736)

Co-Investigator(Renkei-kenkyūsha) ISHIGE Ikuo  東京大学, 医科学研究所, 特任研究員 (30396957)
KOBAYASHI Seiichiro  東京大学, 医科学研究所, 助教 (70376622)
NAGAMURA Fumitaka  東京大学, 医科学研究所, 教授 (90282491)
OOI Jun  東京大学, 医科学研究所, 助教 (20302606)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,270,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2009: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywords再生医療 / 臍帯 / 組織幹細胞 / ES細胞 / CD34陽性細胞 / 間葉系幹細胞 / VSEL / 臍帯血
Research Abstract

This study is aimed to find the source cells for regenerative medicine, such as Very Small ES like(VSEL) cells in umbilical cord blood(UCB). We found the SSEA3^+very small N/C high-ratio cells, but too low incidence to do further analysis. On the other hand, in umbilical cord(UC)-derived mesenchymal stem cells(MSCs), we found the SSEA3/4+cells with ES-specific gene markers. Not only UCB, but also UC-MSCs may be good candidate source for the regenerative medicine.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (35 results)

All 2011 2010 2009

All Journal Article (19 results) (of which Peer Reviewed: 15 results) Presentation (16 results)

  • [Journal Article] Time from cord blood collection to processing and temperature influence the quality of mononuclear cell products isolated using a density-gradient protocol2011

    • Author(s)
      Miki Yuzawa, Nagamura-Inoue T, Ikuo Ishige, Kazuo Ogami, Tomoki Tamura, Atsuko Takahashi, Hideki Kodo, Satoru Yamaguchi, and Arinobu Tojo
    • Journal Title

      The Japan Society of Transfusion Medicine and Cell Therapy.(日本輸血・細胞治療学会誌)

      Volume: 57 Pages: 139-145

    • NAID

      10029418265

    • URL

      http://www.jstmct.or.jp/jstmct/Journal/BackNumber.aspx

    • Related Report
      2011 Final Research Report
  • [Journal Article] Japan Cord Blood Bank Network. Cord Blood Transplantation from Unrelated Donors for Children with Acute Lymphoblastic Leukemia in Japan : The Impact of Methotrexate on Clinical Outcomes.2011

    • Author(s)
      Kato K, Yoshimi A, Ito E, Oki K, Hara J, Nagatoshi Y, Kikuchi A, Kobayashi R, Nagamura-Inoue T, Kai S, Azuma H, Takanashi M, Isoyama K, Kato S
    • Journal Title

      Biol Blood Marrow Transplant.

      Volume: 17 Issue: 12 Pages: 1814-21

    • DOI

      10.1016/j.bbmt.2011.05.013

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Japanese Cord Blood Bank Network. Outcome of unrelated umbilical cord blood transplantation in 88 patients with primary immunodeficiency in Japan2011

    • Author(s)
      Morio T, Atsuta Y, Tomizawa D, Nagamura-Inoue T, Kato K, Ariga T, Kawa K, Koike K, Tauchi H, Kajiwara M, Hara T, Kato S
    • Journal Title

      Br J Haematol

      Volume: 154 Pages: 363-72

    • Related Report
      2011 Final Research Report
  • [Journal Article] Time from cord blood collection to processing and temperature influence the quality of mononuclear cell products isolated using a density-gradient protocol.2011

    • Author(s)
      Miki Yuzawa, Nagamura-Inoue T, Arinobu Tojo, et al
    • Journal Title

      The Japan Society of Transfusion Medicine and Cell Therapy.(日本輸血・細胞治療学会誌)

      Volume: 57 Pages: 139-145

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Outcome of unrelated umbilical cord blood transplantation in 88 patients with primary immunodeficiency in Japan2011

    • Author(s)
      Morio T, Atsuta Y, Tomizawa D, Nagamura-Inoue T, Kato K, et al; Japanese Cord Blood Bank Network
    • Journal Title

      Br J Haematol.

      Volume: 154 Pages: 363-72

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Guideline for processing cellular therapy products routinely used for hematopoietic stem cell transplantation in Japan. The Japan Society of Transfusion Medicine and Cell Therapy2011

    • Author(s)
      Tanosaki R., Muroi K., Nagamura-Inoue T., et al for Cell Processing Guideline Working Group of the Japan Society of Transfusion Medicine, Cell Therapy (JSTMCT)
    • Journal Title

      The Japan Society of Transfusion Medicine and Cell Therapy.(日本輸血・細胞治療学会誌(報告))

      Volume: 57 Pages: 184-187

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytokine production by primary human macrophages infected with highly pathogenic H5N1 or pandemic H1N1 2009 influenza viruses.2011

    • Author(s)
      Sakabe S, Nagamura-Inoue T, et al.
    • Journal Title

      J Gen Virol

      Volume: (印刷中)(掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Outcome of unrelated umbilical cord blood transplantation in 88 patients with primary immunodeficiency in Japan2011

    • Author(s)
      Morio T., Nagamura-Inoue T, et al.
    • Journal Title

      British J of Haematology

      Volume: (印刷中)(掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Time from cord blood collection to processing and temperature influence on the quality of mononuclear cell products isolated using a density-gradient protocol2011

    • Author(s)
      Yuzawa M, Nagamura-Inoue T., et al
    • Journal Title

      The Japan Society of Transfusion Medicine and Cell Therapy

      Volume: (印刷中)(掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transplantation of allogeneic hematopoietic stem cell for adult T cell leukemia : a nationwide retrospective study.2010

    • Author(s)
      Hishizawa M, Nagamura-Inoue T, et al.
    • Journal Title

      Blood

      Volume: 116 Pages: 1369-1376

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term outcome of cord blood transplantation from unrelated donors as an initial transplantation procedure for children with AML in Japan.2010

    • Author(s)
      Isoyama K., Nagamura-Inoue T., et al
    • Journal Title

      Bone Marrow Transplant

      Volume: 45 Pages: 69-77

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Disease-specific analyses of unrelated cord blood transplant compared with unrelated bone marrow transplant in adult patients with acute leukemia2009

    • Author(s)
      Atsuta Y., Suzuki R., Nagamura-Inoue T., Taniguchi S., Takahashi S., Kai S., Sakamaki H., Kouzai Y., Kasai M., Fukuda T., Azuma H., Takanashi M., Okamoto S., Tsuchida M., Kawa K., Morishima Y., Kodera Y., and Kato S.
    • Journal Title

      Blood

      Volume: 113 Issue: 8 Pages: 1631-8

    • DOI

      10.1182/blood-2008-03-147041

    • Related Report
      2011 Final Research Report
  • [Journal Article] Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton's jelly explants of human umbilical cord, Int2009

    • Author(s)
      Ishige I., Nagamura-Inoue T., Honda J. M, Harnprasopwat R., Kido M., Sugimoto M., Nakauchi H, Tojo A
    • Journal Title

      J of Hematology

      Volume: 90 Issue: 2 Pages: 261-9

    • DOI

      10.1007/s12185-009-0377-3

    • Related Report
      2011 Final Research Report
  • [Journal Article] Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton's jelly explants of human umbilical cord.2009

    • Author(s)
      Ishige I., Nagamura-Inoue T., ら
    • Journal Title

      Int.J of Hematology 90

      Pages: 261-9

    • NAID

      10026108647

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Japan Cord Blood Bank Network, Long-term outcome of cord blood transplantation from unrelated donors as an initial transplantation procedure for children with AML in Japan.2009

    • Author(s)
      Isoyama K, Oda M, Kato K, Nagamura-Inoue T, ら
    • Journal Title

      Bone Marrow Transplant 10

      Pages: 69-77

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CD8+ memory T cells predominate over naive T cells in therapy-free CML patients with sustained major molecular response.2009

    • Author(s)
      Usuki K, Nagamura-Inoue T, ら
    • Journal Title

      Leuk Res. 33

      Pages: 164-5

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Japan Cord Blood Bank Network. Incidence and risk factors of early bacterial infections after unrelated cord blood transplantation.2009

    • Author(s)
      Yazaki M, Nagamura-Inoue T, ら
    • Journal Title

      Biol.Blood Marrow Transplant. 15

      Pages: 439-4615

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Questionnaire surveys on cell processing in Japan by the Japanese Society of Transfusion Medicine and Cell Therapy/Japanese Association of Medical Technologists(2007)and by Working Group for Adverse Events of the Transfusion Conference of the University Hospitals(2006).2009

    • Author(s)
      Ikeda K., Nagamura-Inoue T., ら
    • Journal Title

      日本輸血・細胞治療学会誌 55

      Pages: 397-404

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Disease-specific analyses of unrelated cord blood transplant compared with unrelated bone marrow transplant in adult patients with acute leukemia.2009

    • Author(s)
      Atsuta Y., Suzuki R., Nagamura-Inoue, T., ら
    • Journal Title

      Blood 113

      Pages: 1631-8

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 臍帯血からの制御性T細胞の誘導増幅による免疫抑療法の開発2011

    • Author(s)
      長村(井上)登紀子,小林誠一郎,尾上和夫,東條有伸
    • Organizer
      第73回日本血液学会総会
    • Place of Presentation
      OS-3-36
    • Year and Date
      2011-10-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 未分化マーカーSSEA4を発現する臍帯由来MSCsの解析Characterization of human umbilical cord-derived primitive mesenchymal stem cells expressing SSEA42011

    • Author(s)
      何海萍、長村(井上)登紀子、田村友樹、湯沢美紀、東條有伸
    • Organizer
      第73回日本血液学会総会
    • Place of Presentation
      京都
    • Year and Date
      2011-10-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 未分化マーカーSSEA4を発現する臍帯由来間葉系幹細胞の解析Characterization of human umbilical cord-derived primitive mesenchymal stem cells expressing SSEA42011

    • Author(s)
      何海萍, 長村登紀子, 角田肇, 田村智樹, 湯沢美紀, 東條有伸
    • Organizer
      第73回日本血液学会学術集会(総会)
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] DEK/NUP異常(t(6;9)(P23;q34))は急性骨髄性白血病第1寛解期同種移植における予後良好因子である2011

    • Author(s)
      石山謙、長村登紀子, 他
    • Organizer
      日本造血細胞移植学会総会
    • Place of Presentation
      愛媛県民文化会館
    • Year and Date
      2011-03-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 成人T細胞白血病(ArL)の同種造血幹細胞移植におけるドナーのHTLV-1感染の影響2011

    • Author(s)
      菱澤方勝、長村登紀子, 他
    • Organizer
      日本造血細胞移植学会総会
    • Place of Presentation
      愛媛県民文化会館
    • Year and Date
      2011-03-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Changes In Incidence and Causes of Non-Relapse Mortality (NRM) After Allogeneic Hematopoietic Cell Transplantation (allo-HCT) : Are Transplants Improving?2010

    • Author(s)
      Kurosawa S, Nagamura-Inoue T, et al.
    • Organizer
      American Society of Hematology Annual Meeting
    • Place of Presentation
      米国 オーランド
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Unrelated Umbilical Cord Blood Transplantation for Patients with Primary Immunodeficiency : A Report From the Registry of the Japan Cord Blood Bank Network2010

    • Author(s)
      Mori T, Nagamura-Inoue T, et al.
    • Organizer
      American Society of Hematology Annual Meeting
    • Place of Presentation
      米国 オーランド
    • Year and Date
      2010-12-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] DEK/NUP214 Fusion Gene Predicts Good Outcome In Acute Myeloid Leukemia In Complete Remission After Allogeneic Hematopoietic Stem Cell Transplantation2010

    • Author(s)
      Ishiyama K, Nagamura-Inoue T, et
    • Organizer
      American Society of Hematology Annual Meeting
    • Place of Presentation
      米国 オーランド
    • Year and Date
      2010-12-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 制御性T細胞の誘導増幅による免疫抑制療法の基礎的開発2010

    • Author(s)
      長村登紀子、尾上和夫、横山和明、東條有伸
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 東造血細胞移植における細胞検査2010

    • Author(s)
      長村(井上)登紀子
    • Organizer
      第17回日本輸血・細胞治療学会秋季シンポジウム
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 東大医科研における造血幹細胞移植時の輸血対応について2010

    • Author(s)
      和田結花, 長村(井上)登紀子, 他
    • Organizer
      第58回日本輸血・細胞治療学会総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2010-05-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Compatrative study of mesehchymal stem cells derived from arterial, venous, and Wharton's jelly explants of human umbilical cord., International Society of Cell Therapy2010

    • Author(s)
      Ikuo Ishige, Tokiko Nagmaura-Inoue, et al
    • Organizer
      International Society of Cell Therapy, Annual meeting
    • Place of Presentation
      米国 フィラデルフィア
    • Year and Date
      2010-05-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Establishment of standards for processing cellular therapy product routinely used for hematopoietic stem cell transplantation in Janan2009

    • Author(s)
      田野崎隆二、長村登紀子, 等
    • Organizer
      XXth Regional Congress of the International Society of Blood Transfusion(ISBT), Asia
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2009-11-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] 原発性骨髄線維症に対する臍帯血移植2009

    • Author(s)
      村田誠、長村-井上登紀子, 等
    • Organizer
      日本血液学会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2009-10-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] イマチニブはCD8+CD26highメモリーT細胞の機能分化を阻害する2009

    • Author(s)
      横山和明、長村登紀子, 等
    • Organizer
      日本血液学会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] 国内外の臍帯血や造血幹細胞処理基準からふり返ってみた院内細胞処理基準における課題2009

    • Author(s)
      長村登紀子
    • Organizer
      第57回日本輸血・細胞治療学会
    • Place of Presentation
      大宮ソニックシティ さいたま市
    • Year and Date
      2009-05-28
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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