Detection of VSEL cells in umbilical cord blood and development of co-culture system with umbilical cord-derived mesehchymal stem cells

• Project/Area Number: 21659069
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: NAGAMURA Tokiko (INOUE Tokiko) 東京大学, 医科学研究所, 講師 (70240736)
• Co-Investigator(Renkei-kenkyūsha): ISHIGE Ikuo 東京大学, 医科学研究所, 特任研究員 (30396957) ; KOBAYASHI Seiichiro 東京大学, 医科学研究所, 助教 (70376622) ; NAGAMURA Fumitaka 東京大学, 医科学研究所, 教授 (90282491) ; OOI Jun 東京大学, 医科学研究所, 助教 (20302606)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,270,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2009: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: 再生医療 / 臍帯 / 組織幹細胞 / ES細胞 / CD34陽性細胞 / 間葉系幹細胞 / VSEL / 臍帯血

Research Abstract

This study is aimed to find the source cells for regenerative medicine, such as Very Small ES like(VSEL) cells in umbilical cord blood(UCB). We found the SSEA3^+very small N/C high-ratio cells, but too low incidence to do further analysis. On the other hand, in umbilical cord(UC)-derived mesenchymal stem cells(MSCs), we found the SSEA3/4+cells with ES-specific gene markers. Not only UCB, but also UC-MSCs may be good candidate source for the regenerative medicine.

Research Products

• [Journal Article] Time from cord blood collection to processing and temperature influence the quality of mononuclear cell products isolated using a density-gradient protocol (2011)
  • Author(s): Miki Yuzawa, Nagamura-Inoue T, Ikuo Ishige, Kazuo Ogami, Tomoki Tamura, Atsuko Takahashi, Hideki Kodo, Satoru Yamaguchi, and Arinobu Tojo
  • Journal Title: The Japan Society of Transfusion Medicine and Cell Therapy.(日本輸血・細胞治療学会誌) Volume: 57 Pages: 139-145
  • NAID: 10029418265
  • URL: http://www.jstmct.or.jp/jstmct/Journal/BackNumber.aspx
• [Journal Article] Japan Cord Blood Bank Network. Cord Blood Transplantation from Unrelated Donors for Children with Acute Lymphoblastic Leukemia in Japan : The Impact of Methotrexate on Clinical Outcomes. (2011)
  • Author(s): Kato K, Yoshimi A, Ito E, Oki K, Hara J, Nagatoshi Y, Kikuchi A, Kobayashi R, Nagamura-Inoue T, Kai S, Azuma H, Takanashi M, Isoyama K, Kato S
  • Journal Title: Biol Blood Marrow Transplant. Volume: 17 Issue: 12 Pages: 1814-21
  • DOI: 10.1016/j.bbmt.2011.05.013
  • Peer Reviewed
• [Journal Article] Japanese Cord Blood Bank Network. Outcome of unrelated umbilical cord blood transplantation in 88 patients with primary immunodeficiency in Japan (2011)
  • Author(s): Morio T, Atsuta Y, Tomizawa D, Nagamura-Inoue T, Kato K, Ariga T, Kawa K, Koike K, Tauchi H, Kajiwara M, Hara T, Kato S
  • Journal Title: Br J Haematol Volume: 154 Pages: 363-72
• [Journal Article] Time from cord blood collection to processing and temperature influence the quality of mononuclear cell products isolated using a density-gradient protocol. (2011)
  • Author(s): Miki Yuzawa, Nagamura-Inoue T, Arinobu Tojo, et al
  • Journal Title: The Japan Society of Transfusion Medicine and Cell Therapy.(日本輸血・細胞治療学会誌) Volume: 57 Pages: 139-145
  • Peer Reviewed
• [Journal Article] Outcome of unrelated umbilical cord blood transplantation in 88 patients with primary immunodeficiency in Japan (2011)
  • Author(s): Morio T, Atsuta Y, Tomizawa D, Nagamura-Inoue T, Kato K, et al; Japanese Cord Blood Bank Network
  • Journal Title: Br J Haematol. Volume: 154 Pages: 363-72
  • Peer Reviewed
• [Journal Article] Guideline for processing cellular therapy products routinely used for hematopoietic stem cell transplantation in Japan. The Japan Society of Transfusion Medicine and Cell Therapy (2011)
  • Author(s): Tanosaki R., Muroi K., Nagamura-Inoue T., et al for Cell Processing Guideline Working Group of the Japan Society of Transfusion Medicine, Cell Therapy (JSTMCT)
  • Journal Title: The Japan Society of Transfusion Medicine and Cell Therapy.(日本輸血・細胞治療学会誌(報告)) Volume: 57 Pages: 184-187
  • Peer Reviewed
• [Journal Article] Cytokine production by primary human macrophages infected with highly pathogenic H5N1 or pandemic H1N1 2009 influenza viruses. (2011)
  • Author(s): Sakabe S, Nagamura-Inoue T, et al.
  • Journal Title: J Gen Virol Volume: (印刷中)(掲載確定)
  • Peer Reviewed
• [Journal Article] Outcome of unrelated umbilical cord blood transplantation in 88 patients with primary immunodeficiency in Japan (2011)
  • Author(s): Morio T., Nagamura-Inoue T, et al.
  • Journal Title: British J of Haematology Volume: (印刷中)(掲載確定)
  • Peer Reviewed
• [Journal Article] Time from cord blood collection to processing and temperature influence on the quality of mononuclear cell products isolated using a density-gradient protocol (2011)
  • Author(s): Yuzawa M, Nagamura-Inoue T., et al
  • Journal Title: The Japan Society of Transfusion Medicine and Cell Therapy Volume: (印刷中)(掲載確定)
  • Peer Reviewed
• [Journal Article] Transplantation of allogeneic hematopoietic stem cell for adult T cell leukemia : a nationwide retrospective study. (2010)
  • Author(s): Hishizawa M, Nagamura-Inoue T, et al.
  • Journal Title: Blood Volume: 116 Pages: 1369-1376
  • Peer Reviewed
• [Journal Article] Long-term outcome of cord blood transplantation from unrelated donors as an initial transplantation procedure for children with AML in Japan. (2010)
  • Author(s): Isoyama K., Nagamura-Inoue T., et al
  • Journal Title: Bone Marrow Transplant Volume: 45 Pages: 69-77
  • Peer Reviewed
• [Journal Article] Disease-specific analyses of unrelated cord blood transplant compared with unrelated bone marrow transplant in adult patients with acute leukemia (2009)
  • Author(s): Atsuta Y., Suzuki R., Nagamura-Inoue T., Taniguchi S., Takahashi S., Kai S., Sakamaki H., Kouzai Y., Kasai M., Fukuda T., Azuma H., Takanashi M., Okamoto S., Tsuchida M., Kawa K., Morishima Y., Kodera Y., and Kato S.
  • Journal Title: Blood Volume: 113 Issue: 8 Pages: 1631-8
  • DOI: 10.1182/blood-2008-03-147041
• [Journal Article] Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton's jelly explants of human umbilical cord, Int (2009)
  • Author(s): Ishige I., Nagamura-Inoue T., Honda J. M, Harnprasopwat R., Kido M., Sugimoto M., Nakauchi H, Tojo A
  • Journal Title: J of Hematology Volume: 90 Issue: 2 Pages: 261-9
  • DOI: 10.1007/s12185-009-0377-3
• [Journal Article] Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton's jelly explants of human umbilical cord. (2009)
  • Author(s): Ishige I., Nagamura-Inoue T., ら
  • Journal Title: Int.J of Hematology 90 Pages: 261-9
  • NAID: 10026108647
  • Peer Reviewed
• [Journal Article] Japan Cord Blood Bank Network, Long-term outcome of cord blood transplantation from unrelated donors as an initial transplantation procedure for children with AML in Japan. (2009)
  • Author(s): Isoyama K, Oda M, Kato K, Nagamura-Inoue T, ら
  • Journal Title: Bone Marrow Transplant 10 Pages: 69-77
  • Peer Reviewed
• [Journal Article] CD8+ memory T cells predominate over naive T cells in therapy-free CML patients with sustained major molecular response. (2009)
  • Author(s): Usuki K, Nagamura-Inoue T, ら
  • Journal Title: Leuk Res. 33 Pages: 164-5
  • Peer Reviewed
• [Journal Article] Japan Cord Blood Bank Network. Incidence and risk factors of early bacterial infections after unrelated cord blood transplantation. (2009)
  • Author(s): Yazaki M, Nagamura-Inoue T, ら
  • Journal Title: Biol.Blood Marrow Transplant. 15 Pages: 439-4615
  • Peer Reviewed
• [Journal Article] Questionnaire surveys on cell processing in Japan by the Japanese Society of Transfusion Medicine and Cell Therapy/Japanese Association of Medical Technologists(2007)and by Working Group for Adverse Events of the Transfusion Conference of the University Hospitals(2006). (2009)
  • Author(s): Ikeda K., Nagamura-Inoue T., ら
  • Journal Title: 日本輸血・細胞治療学会誌 55 Pages: 397-404
  • Peer Reviewed
• [Journal Article] Disease-specific analyses of unrelated cord blood transplant compared with unrelated bone marrow transplant in adult patients with acute leukemia. (2009)
  • Author(s): Atsuta Y., Suzuki R., Nagamura-Inoue, T., ら
  • Journal Title: Blood 113 Pages: 1631-8
  • Peer Reviewed
• [Presentation] 臍帯血からの制御性T細胞の誘導増幅による免疫抑療法の開発 (2011)
  • Author(s): 長村(井上)登紀子,小林誠一郎,尾上和夫,東條有伸
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: OS-3-36
  • Year and Date: 2011-10-16
• [Presentation] 未分化マーカーSSEA4を発現する臍帯由来MSCsの解析Characterization of human umbilical cord-derived primitive mesenchymal stem cells expressing SSEA4 (2011)
  • Author(s): 何海萍、長村(井上)登紀子、田村友樹、湯沢美紀、東條有伸
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 京都
  • Year and Date: 2011-10-14
• [Presentation] 未分化マーカーSSEA4を発現する臍帯由来間葉系幹細胞の解析Characterization of human umbilical cord-derived primitive mesenchymal stem cells expressing SSEA4 (2011)
  • Author(s): 何海萍, 長村登紀子, 角田肇, 田村智樹, 湯沢美紀, 東條有伸
  • Organizer: 第73回日本血液学会学術集会(総会)
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-05
• [Presentation] DEK/NUP異常(t(6;9)(P23;q34))は急性骨髄性白血病第1寛解期同種移植における予後良好因子である (2011)
  • Author(s): 石山謙、長村登紀子, 他
  • Organizer: 日本造血細胞移植学会総会
  • Place of Presentation: 愛媛県民文化会館
  • Year and Date: 2011-03-09
• [Presentation] 成人T細胞白血病(ArL)の同種造血幹細胞移植におけるドナーのHTLV-1感染の影響 (2011)
  • Author(s): 菱澤方勝、長村登紀子, 他
  • Organizer: 日本造血細胞移植学会総会
  • Place of Presentation: 愛媛県民文化会館
  • Year and Date: 2011-03-09
• [Presentation] Changes In Incidence and Causes of Non-Relapse Mortality (NRM) After Allogeneic Hematopoietic Cell Transplantation (allo-HCT) : Are Transplants Improving? (2010)
  • Author(s): Kurosawa S, Nagamura-Inoue T, et al.
  • Organizer: American Society of Hematology Annual Meeting
  • Place of Presentation: 米国 オーランド
  • Year and Date: 2010-12-07
• [Presentation] Unrelated Umbilical Cord Blood Transplantation for Patients with Primary Immunodeficiency : A Report From the Registry of the Japan Cord Blood Bank Network (2010)
  • Author(s): Mori T, Nagamura-Inoue T, et al.
  • Organizer: American Society of Hematology Annual Meeting
  • Place of Presentation: 米国 オーランド
  • Year and Date: 2010-12-06
• [Presentation] DEK/NUP214 Fusion Gene Predicts Good Outcome In Acute Myeloid Leukemia In Complete Remission After Allogeneic Hematopoietic Stem Cell Transplantation (2010)
  • Author(s): Ishiyama K, Nagamura-Inoue T, et
  • Organizer: American Society of Hematology Annual Meeting
  • Place of Presentation: 米国 オーランド
  • Year and Date: 2010-12-05
• [Presentation] 制御性T細胞の誘導増幅による免疫抑制療法の基礎的開発 (2010)
  • Author(s): 長村登紀子、尾上和夫、横山和明、東條有伸
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-09-24
• [Presentation] 東造血細胞移植における細胞検査 (2010)
  • Author(s): 長村(井上)登紀子
  • Organizer: 第17回日本輸血・細胞治療学会秋季シンポジウム
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2010-09-22
• [Presentation] 東大医科研における造血幹細胞移植時の輸血対応について (2010)
  • Author(s): 和田結花, 長村(井上)登紀子, 他
  • Organizer: 第58回日本輸血・細胞治療学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2010-05-29
• [Presentation] Compatrative study of mesehchymal stem cells derived from arterial, venous, and Wharton's jelly explants of human umbilical cord., International Society of Cell Therapy (2010)
  • Author(s): Ikuo Ishige, Tokiko Nagmaura-Inoue, et al
  • Organizer: International Society of Cell Therapy, Annual meeting
  • Place of Presentation: 米国 フィラデルフィア
  • Year and Date: 2010-05-25
• [Presentation] Establishment of standards for processing cellular therapy product routinely used for hematopoietic stem cell transplantation in Janan (2009)
  • Author(s): 田野崎隆二、長村登紀子, 等
  • Organizer: XXth Regional Congress of the International Society of Blood Transfusion(ISBT), Asia
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-11-16
• [Presentation] 原発性骨髄線維症に対する臍帯血移植 (2009)
  • Author(s): 村田誠、長村-井上登紀子, 等
  • Organizer: 日本血液学会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-10-25
• [Presentation] イマチニブはCD8+CD26highメモリーT細胞の機能分化を阻害する (2009)
  • Author(s): 横山和明、長村登紀子, 等
  • Organizer: 日本血液学会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-10-23
• [Presentation] 国内外の臍帯血や造血幹細胞処理基準からふり返ってみた院内細胞処理基準における課題 (2009)
  • Author(s): 長村登紀子
  • Organizer: 第57回日本輸血・細胞治療学会
  • Place of Presentation: 大宮ソニックシティ さいたま市
  • Year and Date: 2009-05-28