| Project/Area Number |
21659092
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | National Research Institute for Child Health and Development |
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Principal Investigator |
UMEZAWA Akihiro 独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 部長 (70213486)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Junichiro 国立成育医療センター(研究所), 副所長 (60175578)
HATA Junichi 国立成育医療センター(研究所), 名誉総長 (90051614)
TOYODA Masashi (地独)東京都健康長寿医療センター, 研究副部長 (50392486)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,360,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2009: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | 成育医学 / ES細胞 / フィーダー細胞 / 糖鎖 / 培養システム / クラスタリング / 細胞株 / 幹細胞 / 遺伝子解析 / 再生医療 / 細胞移植 |
|---|
| Research Abstract |
Human embryonic stem(ES) cells are difficult to be cultivated in vitro, compared with murine ES cells. In contrast, human embryonal carcinoma(EC) cells can easily be cultivated, compared with human ES cells. In this study, we evaluated human EC cell lines to investigate which human cells can be used as feeder cells. We set specification of human feeder cells in terms of maintenance of human ES cells and preservation of pluripotency. We determined human cell types that are capable of being feeder cells and indeed established human feeder cells.
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