Identification of the host factors involved in the response of macrophage cells to Legionella pneumophila infection
| Project/Area Number |
21659108
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Bacteriology (including Mycology)
|
|---|
| Research Institution | Saga University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | 細胞死 / マクロファージ / レジオネラ / カスパーゼ / 細胞応答 / 細菌 / 遺伝子 |
|---|
| Research Abstract |
To investigate the role of Naip5 in macrophage survival, RAW264 macrophages expressing Naip5 were constructed. Naip5 promoted cytotoxicity in Legionella pneumophila-infected cells. This cytotoxicity was associated with caspase-1. In addition, Naip5 restricted the intracellular growth of L. pneumophila in RAW264 cells. L. pneumophila flagellin was required for cytotoxicity, caspase-1 activation, and restriction of intracellular bacterial growth. Moreover, Naip5 inhibited camptothecin-induced apoptosis in macrophages. These data indicate that Naip5 regulate cell survival by inhibiting apoptosis or by promoting pyroptosis in response to specific cellular signals.
|
Report
(4 results)
Research Products
(19 results)
-
-
-
-
-
-
-
-
[Journal Article] Development of a novel PCR method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections2010
Author(s)
Akiyama T, Miyamoto H, Fukuda K, Sano N, Katagiri N, Shobuike T, Kukita A, Yamashita Y, Taniguchi H, Goto M.
-
Journal Title
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
Volume: 109(5)
Pages: 669-676
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
