| Project/Area Number |
21659108
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Saga University |
|---|
Principal Investigator |
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | 細胞死 / マクロファージ / レジオネラ / カスパーゼ / 細胞応答 / 細菌 / 遺伝子 |
|---|
| Research Abstract |
To investigate the role of Naip5 in macrophage survival, RAW264 macrophages expressing Naip5 were constructed. Naip5 promoted cytotoxicity in Legionella pneumophila-infected cells. This cytotoxicity was associated with caspase-1. In addition, Naip5 restricted the intracellular growth of L. pneumophila in RAW264 cells. L. pneumophila flagellin was required for cytotoxicity, caspase-1 activation, and restriction of intracellular bacterial growth. Moreover, Naip5 inhibited camptothecin-induced apoptosis in macrophages. These data indicate that Naip5 regulate cell survival by inhibiting apoptosis or by promoting pyroptosis in response to specific cellular signals.
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