Mitochondrial DNA analysis for diabetes cohort study

• Project/Area Number: 21659148
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Kyushu University
• Principal Investigator: KANG Dongchon 九州大学, 医学研究院, 教授 (80214716)
• Co-Investigator(Renkei-kenkyūsha): KIYOHARA Yutaka 九州大学, 大学院・医学研究科, 教授 (80161602)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2009: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: ミトコンドリア / ミトコンドリア病 / 遺伝子診断 / 糖尿病 / コホート研究

Research Abstract

The mitochondrial DNA sequences in the two regions(D-loop and ND6) have been determined for over 3 thousand residents in Hisayama town, Fukuoka prefecture. We have found that one single nucleotide polymorphism(SNP) is statistically linked to a metabolic syndrome. We have established the cybrid cell lines which contain the SNP and now we plan to analyze their mitochondrial activities, IGF response, and so on.

Research Products

• [Journal Article] mtSSB may sequester UNGl at mitochondrial ssDNA and delay uracil processing until the dsDNA conformation is restored (2012)
  • Author(s): Wollen Steen, K., Doseth, B., M, P. W., Akbari, M., Kang, D., Falkenberg, M., Slupphaug, G.
  • Journal Title: DNA Repair(Amst) Volume: 11 Pages: 82-91
• [Journal Article] Arg 901 in the AE1 Cterminal tail is involved in conformational change but not in substrate binding (2012)
  • Author(s): Takazaki, S., Abe, Y., Yamaguchi, T., Yagi, M., Ueda, T., Kang, D., Hamasaki, N.
  • Journal Title: Biochim Biophys Acta Volume: 1818 Pages: 658-665
• [Journal Article] Effect of mitochondrial transcription factor a over expression on motor neurons in amyotrophic lateral sclerosis model mice (2012)
  • Author(s): Morimoto, N., Miyazaki, K., Kurata, T., Ikeda, Y., Matsuura, T., Kang, D., Ide, T., Abe, K.
  • Journal Title: J Neurosci Res Volume: 90 Pages: 1200-1208
• [Journal Article] Ribonucleoprotein Yboxbinding protein-l regulates mitochondrial oxidative phosphorylation(OXPHOS) protein expression after serum stimulation through binding to OXPHOS mRNA (2012)
  • Author(s): Matsumoto, S., Uchiumi, T., Tanamachi, H., Saito, T., Yagi, M., Takazaki, S., Kanki, T., Kang, D.
  • Journal Title: Biochem J Volume: 443 Pages: 573-584
• [Journal Article] Localization of mRNAs encoding human mitochondrial oxidative phosphorylation proteins (2012)
  • Author(s): Matsumoto, S., Uchiumi, T., Saito, T., Yagi, M., Takazaki, S., Kanki, T., Kang, D.
  • Journal Title: Mitochondrion Volume: 12 Pages: 391-398
• [Journal Article] Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast (2012)
  • Author(s): Kurihara, Y., Kanki, T., Aoki, Y., Hirota, Y., Saigusa, T., Uchiumi, T., Kang, D.
  • Journal Title: J Biol Chem Volume: 287 Pages: 3265-3272
• [Journal Article] Ribonucleoprotein Y-box binding protein-1 regulates mitochondrial oxidative phosphorylation (OXPHOS) protein expression after serum stimulation through binding to OXPHOS mRNA (2012)
  • Author(s): Matsumoto S, Uchiumi T, et al
  • Journal Title: Biochemical J Volume: 443 Issue: 2 Pages: 573-584
  • DOI: 10.1042/bj20111728
  • Peer Reviewed
• [Journal Article] Effect of mitochondrial transcription factor a overexpression on motor neurons in amyotrophic lateral sclerosis model mice (2012)
  • Author(s): Morimoto, N., Miyazaki, K., Kurata, T., Ikeda, Y., Matsuura, T., Kang, D., Ide, T., Abe, K.
  • Journal Title: J Neurosci Res Volume: 90 Issue: 6 Pages: 1200-12008
  • DOI: 10.1002/jnr.23000
  • Peer Reviewed
• [Journal Article] The role of TFAM-associated proteins in mitochondrial RNA metabolism (2012)
  • Author(s): Uchiumi T, Kang D
  • Journal Title: Biochim Biophys Acta Volume: 1820 Issue: 5 Pages: 565-570
  • DOI: 10.1016/j.bbagen.2011.08.014
  • Peer Reviewed
• [Journal Article] mtSSB may sequester UNG1 at mitochondrial ssDNA and delayuracil processing until the dsDNA conformation is restored (2012)
  • Author(s): Wollen Steen, K., Doseth, B., M, P.W., Akbari, M., Kang, D., Falkenberg, M., Slupphaug, G.
  • Journal Title: DNA Repair (Amst) Volume: 11 Pages: 82-91
  • Peer Reviewed
• [Journal Article] Frequent Truncating Mutation of TFAM Induces Mitochondrial DNA Depletion and Apoptotic Resistance in MicrosatelliteUnstable Colorectal Cancer (2011)
  • Author(s): Guo, J., Zheng, L., Lin, W., Wang, X., Wang, Z., Wang, Z., French, A. J., Kang, D., Chen, L., Thibodeau, S. N., Lin, W.
  • Journal Title: Cancer Res Volume: 71 Pages: 2978-2987
• [Journal Article] Phosphorylation of Seline 114 on Atgs2 mediates mitophagy (2011)
  • Author(s): Aoki, Y., Kanki, T., Hirota, Y., Kurihara, Y., Saigusa, T., Uchiumi, T., Kang, D.
  • Journal Title: Mol Biol Cell Volume: 22 Pages: 3206-3217
• [Journal Article] Mitochondrial p32/ClQBP is highly expressed in prostate cancer and is associated with shorter prostatespecific antigen relapse time after radical prostatectomy (2011)
  • Author(s): Amamoto, R., Yagi, M., Song, Y., Oda, Y., Tsuneyoshi, M., Naito, S., Yokomizo, A., Kuroiwa, K., Tokunaga, S., Kate, S., Hiura, H., Samori, T., Kang, D., Uchiumi, T.
  • Journal Title: Cancer Sci Volume: 102 Pages: 639-647
• [Journal Article] Phosphorylation of Serine 114 on Atg32 mediates mitophagy (2011)
  • Author(s): Aoki, Y., Kanki, T., Hirota, Y., Kurihara, Y., Saigusa, T., Uchiumi, T., Kang, D.
  • Journal Title: Mol Biol Cell Volume: 22 Issue: 17 Pages: 3206-3217
  • DOI: 10.1091/mbc.e11-02-0145
  • Peer Reviewed
• [Journal Article] Frequent Truncating Mutation of TFAM Induces Mitochondrial DNA Depletion and Apoptotic Resistance in Microsatellite-Unstable Colorectal Cancer (2011)
  • Author(s): Guo, J., Zheng, L., Liu, W., Wang, X., Wang, Z., Wang, Z., French, A.J., Kang, D., Chen, L., Thibodeau, S.N., Liu, W.
  • Journal Title: Cancer Res Volume: 71 Issue: 8 Pages: 2978-2987
  • DOI: 10.1158/0008-5472.can-10-3482
  • Peer Reviewed
• [Journal Article] Structure of the membrane domain of human erythrocyte anion exchanger 1 revealed by electron crystallography (2010)
  • Author(s): Yamaguchi, T., Ikeda, Y., Abe, Y., Kuma, H., Kang, D., Hamasaki, N., Hirai, T.
  • Journal Title: J Mol Biol Volume: 397 Pages: 179-189
• [Journal Article] Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals (2010)
  • Author(s): Yamaguchi, T., Fu j i i, T., Abe, Y., Hirai, T., Kang, D., Namba, K., Hamasaki, N., Mitsuoka, K.
  • Journal Title: J Struct Biol Volume: 169 Pages: 406-412
• [Journal Article] ERALl is associated with mitochondrial ribosome and elimination of ERALl leads to mitochondrial dysfunction and growth retardation (2010)
  • Author(s): Uchiumi, T., Ohgaki, K., Yagi, M., Aoki, Y., Sakai, A., Matsumoto, S., Kang, D.
  • Journal Title: Nucleic Acids Res Volume: 38 Pages: 5554-5568
• [Journal Article] Geographic distribution of fluoroquinolone resistant Escherichia coll strains in Asia (2010)
  • Author(s): Uchida, Y., Mochimaru, T., Morokuma, Y., Kiyosuke, M., Fujise, M., Eta, F., Harada, Y., Kadowaki, M., Shimono, N., Kang, D.
  • Journal Title: Int j Antimicrob Agents Volume: 35 Pages: 387-391
• [Journal Article] Clonal spread in Eastern Asia of ciprofloxacin resistant Escherichia coll serogroup 025 strains, and associated virulence factors (2010)
  • Author(s): Uchida, Y., Mochimaru, T., Morokuma, Y., Kiyosuke, M., Fujise, M., Eta, F., Eriguchi, Y., Nagasaki, Y., Shimono, N., Kang, D.
  • Journal Title: Int J Antimicrob Agents Volume: 35 Pages: 444-450
• [Journal Article] Mutation of His 834 in human anion exchanger 1 affects substrate binding (2010)
  • Author(s): Takazaki, S., Abe, Y., Yamaguchi, T., Yagi, M., Ueda, T., Kang, D., Hamasaki, N.
  • Journal Title: Biochim Biophys Acta Volume: 1798 Pages: 903-908
• [Journal Article] PLEDs in an infant with congenital protein C deficiency : a case report (2010)
  • Author(s): Sekiguchi, K., Akiyoshi, K., Okazaki, N., Yamada, H., Suzuki, M., Maeda, T., Suenobu, S., Izumi, T., Kang, D.
  • Journal Title: Crin Neurephysiol Volume: 121 Pages: 800-801
• [Journal Article] IFCC reference procedures for measurement of the catalytic concentrations of enzymes : corrigendum, notes and useful advice (2010)
  • Author(s): Schumann, G., Can alias, F., Joergensen, P. J., Kang, D., Lessinger, J. M., Klauke, R.
  • Journal Title: Crin Chem Lab Med Volume: 48 Pages: 615-621
• [Journal Article] (2010) Mitochondrial singlestranded DNA binding protein is required for maintenance of mitochondrial DNA and 7S DNA but is not required for mitochondrial nucleoid organisation (2010)
  • Author(s): Ruhanen, H., Berrie, S., Szabadka', G., Tyynismaa, H., Jones, A. W., Kang, D., Taanman, J. W., Yasukawa, T.
  • Journal Title: Biochim Biophys Acta Volume: 1803 Pages: 931-939
• [Journal Article] Structure of the membrane domain of human erythrocyte anion exchanger 1 revealed by electron crystallography. (2010)
  • Author(s): Yamaguchi, T., Ikeda, Y., Abe, Y., Kuma, H., Kang, D., Hamasaki, N., Hirai, T.
  • Journal Title: J Mol Biol Volume: 397 Pages: 179-189
  • Peer Reviewed
• [Journal Article] ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation (2010)
  • Author(s): Uchiumi, T., Ohgaki, K., Yagi, M., Aoki, Y., Sakai, A., Matsumoto, S., Kang, D
  • Journal Title: Nucleic Acids Res Volume: 38 Pages: 5554-5568
  • Peer Reviewed
• [Journal Article] Geographic distribution of fluoroquinolone-resistant Escherichia coli strains in Asia (2010)
  • Author(s): Uchida, Y., Mochimaru, T., Morokuma, Y., Kiyosuke, M., Fujise, M., Eto, F., Harada, Y., Kadowaki, M., Shimono, N., Kang, D.
  • Journal Title: Int J Antimicrob Agents Volume: 35 Pages: 387-391
  • Peer Reviewed
• [Journal Article] Clonal spread in Eastern Asia of ciprofloxacin-resistant Escherichia coli serogroup O25 strains, and associated virulence factors. (2010)
  • Author(s): Uchida, Y., Mochimaru, T., Morokuma, Y., Kiyosuke, M., Fujise, M., Eto, F., Eriguchi, Y. Nagasaki, Y., Shimono, N., Kang, D.
  • Journal Title: Int J Antimicrob Agents Volume: 35 Pages: 444-450
  • Peer Reviewed
• [Journal Article] PLEDs in an infant with congenital protein C deficiency : a case report (2010)
  • Author(s): Sekiguchi, K., Akiyoshi, K., Okazaki, N., Yamada, H., Suzuki, M., Maeda, T., Suenobu, S., Izumi, T., Kang, D.
  • Journal Title: Clin Neurophysiol Volume: 121 Pages: 800-801
  • Peer Reviewed
• [Journal Article] IFCC reference procedures for measurement of the catalytic concentrations of enzymes : corrigendum, notes and useful advice (2010)
  • Author(s): Schumann, G., Canalias, F., Joergensen, P.J., Kang, D., Lessinger, J.M., Klauke, R
  • Journal Title: Clin Chem Lab Med Volume: 48 Pages: 615-621
  • Peer Reviewed
• [Journal Article] Mitochondrial single-stranded DNA binding protein is required for maintenance of mitochondrial DNA and 7S DNA but is not required for mitochondrial nucleoid organisation (2010)
  • Author(s): Ruhanen, H., Borrie, S., Szabadkai, G., Tyynismaa, H., Jones, A.W., Kang, D., Taanman, J.W., Yasukawa, T
  • Journal Title: Biochim Biophys Acta Volume: 1803 Pages: 931-939
  • Peer Reviewed
• [Journal Article] Mutation of His 834 in human anion exchanger 1 affects substrate binding. Biochim (2010)
  • Author(s): Takazaki, S., et al.
  • Journal Title: Biochim Biophys Acta 1798 Pages: 903-908
  • Peer Reviewed
• [Journal Article] Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals (2010)
  • Author(s): Yamaguchi, T., et al.
  • Journal Title: J Struct Biol 169 Pages: 406-412
  • Peer Reviewed
• [Journal Article] Structure of the membrane domain of human erythrocyte anion exchanger 1 revealed by electron crystallography (2010)
  • Author(s): Yamaguchi, T., et al.
  • Journal Title: J Mol Biol 397 Pages: 179-189
  • Peer Reviewed
• [Journal Article] Association of a novel mitochondrial protein Mtg with mitochondrial nucleoids (2009)
  • Author(s): Sumitani, M., Kasashima, K., Ohta, E., Kang, D., Endo, H.
  • Journal Title: J Biochem Volume: 146 Pages: 725-732
• [Journal Article] Human heart mitochondrial DNA is organized in complex catenated networks containing abundant four-way junctions and replication forks (2009)
  • Author(s): Pohjoismaki, J. L., Goffart, S., Tyynismaa, H., Willcox, S., Ide, T., Kang, D., Suomalainen, A., Karhunen, P. J., Griffith, J. D., Holt, I. J., Jacobs, H. T.
  • Journal Title: J Biol Chem Volume: 284 Pages: 21446-21457
• [Journal Article] High-dose penicillin G treatment causes under estimation of serum albumin measured by a modified BCP method (2009)
  • Author(s): Ono, M., Aoki, Y., Masumoto, M., Hotta, T., Uchida, Y., Kayamori, Y., Kang, D.
  • Journal Title: Crin. Chim. Acta Volume: 407 Pages: 75-76
• [Journal Article] Fulminant sepsis/meningitis due to Haemophilus influenzae in a protein Cdeficient heterozygote treated with activated protein C therapy (2009)
  • Author(s): Ishimura, M., Saito, M., Ohga, S., Hoshina, T., Baba, H., Urata, M., Kira, R., Takada, H., Kusuhara, K., Kang, D., Hara, T.
  • Journal Title: Eur J Pediatr Volume: 168 Pages: 673-7
• [Journal Article] Development of a. new measurement method for serum calcium with chlorophosphonazo-III (2009)
  • Author(s): Hokazono, E., Osawa, S., Nakano, T., Kawamoto, Y., Oguchi, Y., Hotta, T., Kayamori, Y., Kang, D., Che, Y., Shiba, K., Sate, K.
  • Journal Title: Ann Crin Biochem Volume: 46 Pages: 296-301
• [Journal Article] DNA conformationdependent activities of human mitochondrial RNA polymerase (2009)
  • Author(s): Fukuoh, A., Ohgaki, K., Hatae, H., Kuraoka, I., Aoki, Y., Uchiumi, T., Jacobs, H. T., Kang, D.
  • Journal Title: Genes Cells Volume: 14 Pages: 1029-1042
• [Journal Article] DNA conformation-dependent activities of human mitochondrial RNA polymerase (2009)
  • Author(s): Fukuoh, A., et al.
  • Journal Title: Genes Cells 14 Pages: 1029-1042
  • Peer Reviewed
• [Journal Article] Development of a new measurement method for serum calcium with chiorophosphonazo-III (2009)
  • Author(s): Hokazono, E., et al.
  • Journal Title: Ann Clin Biochem 46 Pages: 296-301
  • Peer Reviewed
• [Journal Article] Fulminant sepsis/meningitis due to Haemophilus influenzae in a protein C-deficient heterozygote treated with activated protein C therapy (2009)
  • Author(s): Ishimura, M., et al.
  • Journal Title: Eur J Pediatr 168 Pages: 673-677
  • Peer Reviewed
• [Journal Article] High-dose penicillin G-treatment causes underestimation of serum albumin measured by a modified BCP method (2009)
  • Author(s): Ono, M., et al.
  • Journal Title: Clin. Chim. Acta 407 Pages: 75-76
  • Peer Reviewed
• [Journal Article] Human heart mitochondrial DNA is organized in complex catenated net works containing abundant four-way functions and replication forks (2009)
  • Author(s): Pohjoismaki, J.L., et al.
  • Journal Title: J Biol Chem 284 Pages: 21446-21457
  • Peer Reviewed
• [Journal Article] Association of a novel mitochondrial protein M19 with mitochondrial nucleoids (2009)
  • Author(s): Sumitani, M., et al.
  • Journal Title: J Biochem 146 Pages: 725-732
  • Peer Reviewed
• [Presentation] Implications of mitochondrial oxidative stress in common diseases (2012)
  • Author(s): Dongchon Kang(Invited speaker)
  • Organizer: International symposium of mitochondrial biomedicine & ASMRM Council Meeting
  • Place of Presentation: Hangzhou, China
  • Year and Date: 2012-04-08
• [Presentation] ミトコンドリアゲノムと酸化ストレス: TFAMから見る細胞機能 (2012)
  • Author(s): 康東天
  • Organizer: 第22回生物試料分析科学会年次学術集会
  • Place of Presentation: 福岡
• [Presentation] ミトコンドリアゲノムと酸化ストレス:TFAMから見る細胞機能 (2012)
  • Author(s): 康東天
  • Organizer: 第22回生物試料分析科学会年次学術集会
  • Place of Presentation: 福岡
• [Presentation] MayTurner症候群に関連する血栓性閉塞と良好な下腹壁静脈の副側血行を呈した先天的AT欠損の若年症例 (2011)
  • Author(s): 藤波舞、伊藤和史、高岡理恵、羽室雅夫、篠原未帆、浦田美秩代、康東天、濱崎直孝
  • Organizer: 第195回日本内科学会近畿地方会
  • Place of Presentation: 大阪
  • Year and Date: 2011-09-10
• [Presentation] Importance of Mitochondrial genome in cellular functions : Oxidative stress and TFAM (2011)
  • Author(s): Dongchon Kang(Keynote lecture)
  • Organizer: Mckay International symposium On mitochondria' medicine in human health and diseases
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2011-07-09
• [Presentation] Importance of Mitochondria 1 genome in cellular functions : Oxidative stress and TFAM (2011)
  • Author(s): D.Kang
  • Organizer: 2011Mckay International symposium On mitochondrial medicine in human health and diseases
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2011-07-09
• [Presentation] 生化学検査標準化と共通基準範囲に向けて (2011)
  • Author(s): 康東天
  • Organizer: 第8回日本医学会総会
  • Place of Presentation: 東京
• [Presentation] 生化学検査標準化と共通基準範囲に向けて (2011)
  • Author(s): 康東天
  • Organizer: 日常検査の現状と将来
  • Place of Presentation: 東京
• [Presentation] Phosphorylation of ser114 on ATG32 mediates mitophagy (2011)
  • Author(s): Tomotake Kanki, Yoshimasa Aoki, Yusuke Kurihara, Tetsu Saigusa, Takeshi Uchiumi, Dongchon Kane
  • Organizer: The 8th Conference of Asian Society for Mitochondrial Research and Medicine
  • Place of Presentation: Kagoshima, Japan
• [Presentation] Recombinant TFAM attenuates pathological remodeling in cardiac myocytes via inhibiting NAFAT signaling (2011)
  • Author(s): Takeo Fujino, Tomomi Ide, Masayoshi Yoshida, Atushi Tanaka, Ken Onitsuka, Yuko Hata, Taka Shinya Takazaki, Mothiohiro Nishida, Dongchon Kang, Kenji Sunagawa
  • Organizer: The 8th Conference of Asian Society for Mitochondrial Research and Medicine
  • Place of Presentation: Kagoshima, Japan
• [Presentation] ミトコンドリアDNA量と生体ホメオスターシスの維持に関するマウスモデルによる検討 (2011)
  • Author(s): 井手友美、康東夫
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] TFAM-associated proteins that are involved in mitochondrial RNA metabolisms (2010)
  • Author(s): Kang, D.
  • Organizer: The 7th Conference of Asian Society for Mitochondrial Research and Medicine
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2010-12-16
• [Presentation] Aging and mitochondrial dysfunction (2010)
  • Author(s): Kang, D.
  • Organizer: The 11th annual meeting for Southern district of the Korean Society for Laboratory Medicine
  • Place of Presentation: Pusan, Korea
  • Year and Date: 2010-06-18
• [Presentation] TFAM-associated proteins that are involved in mitochondr. ial RNA metabolisms (2010)
  • Author(s): Dongchon Kang(Invited speaker)
  • Organizer: The 7th Conference of Asian Society for Mitochondrial Research and Medicine
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Aging and mitochondrial dysfunction (2010)
  • Author(s): Dongchon Kang(Invited speaker)
  • Organizer: The llth annual lodge meeting for Southern district of the Korean Society for Laboratory Medicine
  • Place of Presentation: Pusan, Korea
• [Presentation] Importance of Mitochondrial Genome in Cellular Functions (2009)
  • Author(s): Dongchon Kang(Invited speaker)
  • Organizer: The 21st Annual Meeting of the Korean Society of Molecular and Cellular Biology(KSMCB)
  • Place of Presentation: Seoul, Korea
• [Presentation] ミトコンドリア病とミトコンドリアDNA (2009)
  • Author(s): 康東天
  • Organizer: 第49回日本臨床化学会年次学術集会
  • Place of Presentation: 長崎
• [Presentation] Mitochondria : genome, ROS, and cellular functions (2009)
  • Author(s): Dongchon Kang(Invited speaker)
  • Organizer: SFRR(Society of Free Radical Research) International Free Radical School in Japan
  • Place of Presentation: Yuzawa, Niigata
• [Book] Importance of mitochondrial genome in multiple cellular functions : A central role of TFAM in its maintenance (2011)
  • Author(s): Kang, D., (Ed.)
  • Total Pages: 20
  • Publisher: Research Signpost, Kerala
• [Remarks]
  • URL: http://www.med.kyushu-u.ac._jp/cclm/
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/cclm/
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/cclm/kyositu/index.html