miRNA profiling and proteomic analysis for constructing model cell lines for cancer development

• Project/Area Number: 21659160
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Hygiene
• Research Institution: Wakayama Medical University
• Principal Investigator: MURE Kanae 和歌山県立医科大学, 医学部, 講師 (90268491)
• Co-Investigator(Kenkyū-buntansha): TAKESHITA Tatsuya 和歌山県立医科大学, 医学部, 公衆衛生学教授 (20150310)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2009: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: 予防医学 / マイクロRNA / アポトーシス / 紫外線 / 発がん予防 / 細胞内シグナル伝達 / 食品由来物質 / 突然変異 / DNA修復 / シグナル伝達

Research Abstract

To construct model cell lines for cancer development, profiling the microRNA, a small non-coding RNA (less than 22 bases) which has a function of regulating gene expression, and proteomic analysis were performed. Human keratinocytes were exposed to UV-B, and miRNA related to apoptosis (programming cell death) and DNA repair were analyzed.

Research Products

• [Journal Article] Inverse association of habitual coffee consumption with metabolic syndrome-related biomarkers involving adiponectin (2013)
  • Author(s): Mure K, Maeda S, Mukoubayashi C, Mugitani K, Iwane M, Kinoshita F, Mohara O and Takeshita T
  • Journal Title: Nutrition Volume: (in press)
  • Peer Reviewed
• [Journal Article] Relationship Between Coffee Consumption and Prevalence of Metabolic Syndrome Among Japanese Civil Servants (2012)
  • Author(s): Matsuura H, Mure K, Nishio N, Kitano N, Nagai N and Takeshita T
  • Journal Title: Journal of Epidemiology Volume: 22 Issue: 2 Pages: 160-166
  • DOI: 10.2188/jea.JE20110068
  • NAID: 10030213606
  • ISSN: 0917-5040, 1349-9092
  • Peer Reviewed
• [Journal Article] Skin sensitization study by a new qualitative structure-toxicity relationships (QSTR) approach : K-step Yard sampling (KY) methods (2012)
  • Author(s): Sato K, Umemura T, Tamura T, Kusaka Y, Aoyama K, Ueda A, Harada K, Minamoto K, Otsuki T, Yamashita K, Haitsuka M, Takeshita T, Shibata E, Dobashi K, Kameo S, Miyagawa M, Kaniwa M, Yoshida T, Fukushima T,Yuta K
  • Journal Title: Journal of Oral Tissue Engineering Volume: 9 Pages: 167-173
  • NAID: 130004941182
  • Peer Reviewed
• [Journal Article] Development of gastric cancer in nonatrophic stomach with highly active inflammation identified by serum levels of pepsinogen and Helicobacter pylori antibody together with endoscopic rugal hyperplastic gastritis (2012)
  • Author(s): Watanabe M, Kato J, Inoue I, Yoshimura N, Yoshida T, Mukoubayashi C, Deguchi H, Enomoto S, Ueda K, Maekita T, Iguchi M, Tamai H, Utsunomiya H, Yamamichi N, Fujishiro M, Iwane M, Tekeshita T, Mohara O, Ushijima T, Ichinose M
  • Journal Title: Int J Cancer Volume: 131巻 Issue: 11 Pages: 2632-2642
  • DOI: 10.1002/ijc.27514
  • Peer Reviewed
• [Journal Article] アルコールと生活習慣病予防.分子疫学研究の成果とその応用 (2012)
  • Author(s): 竹下達也
  • Journal Title: 公衆衛生 Volume: 76 Pages: 205-209
• [Journal Article] Does the Japanese Society for Hygiene need its own Code of Conduct?-A comparison of questionnaire between councilors and junior members- (2011)
  • Author(s): Ikeda W, Inaba Y, Takeshita T, Yoshida K,Ogoshi K, Okamoto K
  • Journal Title: Environ Health Prev Med Volume: 16巻 Issue: 3 Pages: 178-184
  • DOI: 10.1007/s12199-010-0175-9
  • Peer Reviewed
• [Journal Article] Elevated risk of colorectal adenoma with Helicobacter pylori-related chronic gastritis (2011)
  • Author(s): Inoue I, Mukoubayashi C, Yoshimura N, Niwa T, Deguchi H, Watanabe M, Enomoto S, Maekita T, Ueda K, Iguchi M, Yanaoka K, Tamai H, Arii K, Oka M, Fujishiro M, Takeshita T, Iwane M, Mohara O, Ichinose M
  • Journal Title: a population-based case-control study Volume: 129巻 Issue: 11 Pages: 2704-2711
  • DOI: 10.1002/ijc.25931
  • Peer Reviewed
• [Journal Article] Autopsy Cases of Lung Cancer and Liver Cancer among Japanese Dentists-A Review of the Annual of the Pathological Autopsy Cases in Japan (2011)
  • Author(s): Nishio N, Tanaka H, Nishio J, Kouda K and Takeshita T
  • Journal Title: Industrial Health Volume: 49 Issue: 5 Pages: 663-671
  • DOI: 10.2486/indhealth.MS943
  • NAID: 130004828650
  • ISSN: 0019-8366, 1880-8026
  • Peer Reviewed
• [Journal Article] Elevated risk of colorectal adenoma with Helicobacter pylori-related chronic gastritis : a population-based case-control study (2011)
  • Author(s): Inoue I, et al
  • Journal Title: Int J Cancer Volume: 129 Pages: 663-671
  • Peer Reviewed
• [Journal Article] 植物由来物質による紫外線誘発細胞障害抑制効果について (2010)
  • Author(s): 牟礼佳苗, 竹下達也
  • Journal Title: 環境システム計測制御学会誌 Volume: 15 Pages: 9-12
  • NAID: 10026688360
• [Journal Article] Association of ALDH2 Genotypes with Periodontitis Progression (2010)
  • Author(s): Nishida N , Tanaka M, Sekine S, Takeshita T, Nakayama K, Morimoto K, Shizukuishi S
  • Journal Title: J Dental Res Volume: 89巻 Issue: 2 Pages: 138-142
  • DOI: 10.1177/0022034509356045
  • Peer Reviewed
• [Journal Article] Preventive effects of etodolac, a selective cyclooxygenase-2 inhibitor, on cancer development in extensive metaplastic gastritis, a Helicobacter pylori-negative precancerous lesion (2010)
  • Author(s): Yanaoka K, Oka M, Yoshimura N, Deguchi H, Mukoubayashi C, Enomoto S, Maekita T, Inoue I, Ueda K, Utsunomiya H, Iguchi M, Tamai H, Fujishiro M, Nakamura Y, Tsukamoto T, Inada K, Takeshita T, Ichinose M
  • Journal Title: Int J Cancer Volume: 126巻 Issue: 6 Pages: 1467-1473
  • DOI: 10.1002/ijc.24862
  • Peer Reviewed
• [Journal Article] A respiratory sensitization study by a new quantitative structure- activity relationships (QSAR) (2010)
  • Author(s): Sato K, Umemura T, Tamura T, Kusaka Y, Ido T, Aoyama K, Ueda A, Harada K, Minamoto K, Otsuki T, Yamashita K, Takeshita T, Shibata E, Dobashi K, Kameo S, Miyagawa M, Kaniwa M, Yoshida T, Fukushima T and Yuta K
  • Journal Title: Alternatives to Animal Testing and Experimentation Volume: 15 Pages: 124-130
  • NAID: 10029747707
  • Peer Reviewed
• [Journal Article] Upregulation of Fractalkine and Its Receptor, CX3CR1, is Associated With Coronary Plaque Rupture in Patients With Unstable Angina Pectoris (2010)
  • Author(s): Ikejima H, Imanishi T, Tsujioka H, Kashiwagi M, Kuroi A, Tanimoto T, Kitabata H, Ishibashi K, Komukai K, Takeshita T and Akasaka T
  • Journal Title: Circulation Journal Volume: 74 Issue: 2 Pages: 337-345
  • DOI: 10.1253/circj.CJ-09-0484
  • NAID: 10025942643
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed
• [Journal Article] Association of monocyte subset counts with coronary fibrous cap thickness in patients with unstable angina pectoris (2010)
  • Author(s): Imanishi T, Ikejima H, Tsujioka H, Kuroi A, Ishibashi K, Komukai K, Tanimoto T, Ino Y, Takeshita T and Akasaka T
  • Journal Title: Atherosclerosis Volume: 20 Pages: 628-35
  • Peer Reviewed
• [Journal Article] First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor (2010)
  • Author(s): Takarada S, Imanishi T, Goto M, Mochizuki S, Ikejima H, Tsujioka H, Kuroi A, Takeshita T, Akasaka T
  • Journal Title: Eur Heart J Volume: 31巻 Issue: 23 Pages: 2862-2870
  • DOI: 10.1093/eurheartj/ehq156
  • Peer Reviewed
• [Journal Article] 植物由来物質による紫外線誘発細胞障害抑制効果について (2010)
  • Author(s): 牟礼佳苗
  • Journal Title: EICA Volume: 2-3 Pages: 9-12
  • NAID: 10026688360
• [Journal Article] Association of ALDH2 genotypes with periodontitis progression (2010)
  • Author(s): Nishida, N
  • Journal Title: J Dental Res Volume: 89 Pages: 138-142
  • Peer Reviewed
• [Journal Article] Upregulation of fractalkine and its receptor, CX3CR1, is associated with coronary plaque rupture in patients with unstable angina pectoris (2010)
  • Author(s): Ikejima H
  • Journal Title: Circ J Volume: 74 Pages: 337-345
  • Peer Reviewed
• [Journal Article] Smoking Prevalence among Dentists in Hyogo, Japan 2003 (2009)
  • Author(s): Nishio N, Kouda K, Nishio J, Nakamura H, Sonoda Y and Takeshita T
  • Journal Title: Industrial Health Volume: 47 Issue: 4 Pages: 431-435
  • DOI: 10.2486/indhealth.47.431
  • NAID: 130004483395
  • ISSN: 0019-8366, 1880-8026
  • Peer Reviewed
• [Journal Article] Eradication of Helicobacter pylori prevents cancer development in subjects with mild gastric atrophy identified by serum pepsinogen levels (2009)
  • Author(s): Yanaoka K, Oka M, Ohata H, Yoshimura N, Deguchi H, Mukoubayashi C, Enomoto S, Inoue I, Iguchi M, aekita T, Ueda K, Utsunomiya H, Tamai H, Fujishiro M, Iwane M, Takeshita T, Mohara O, Ichinose M
  • Journal Title: International Journal of Cancer Volume: 125 Issue: 11 Pages: 2697-2703
  • DOI: 10.1002/ijc.24591
  • Peer Reviewed
• [Journal Article] Paradoxical increases in serum levels of highly chlorinated PCBs in aged women in clear contrast to robust decreases in dietary intakes from 1980 to 2003 in Japan (2009)
  • Author(s): Koizumi A, Harada KH, Eslami B, Fujimine Y, Hachiya N, Hirosawa I, Inoue K, Inoue S, Koda S, Kusaka Y, Murata K, Omae K, Saito N, Shimbo S, Takenaka K, Takeshita T, Todoriki H, Wada Y, Watanabe T, Ikeda M
  • Journal Title: Environmental Health and Preventive Medicine Volume: 14 Issue: 4 Pages: 235-246
  • DOI: 10.1007/s12199-009-0089-6
  • NAID: 10026959024
  • Peer Reviewed
• [Journal Article] Skin sensitization study by quantitative structureactivity relationships (QSAR) (2009)
  • Author(s): Sato K, Umemura T, Tamura T, Kusaka Y, Aoyama K, Ueda A, Harada K, Minamoto K, Otsuki T, Yamashita K, Takeshita T, Shibata E, Dobashi K, Kameo S, Miyagawa M, Kaniwa M, Endo Y and Yuta K
  • Journal Title: Alternatives to Animal Testing and Experimentation Volume: 14 Pages: 940-946
  • Peer Reviewed
• [Journal Article] 血圧と血中抗酸化能に及ぼす運動の影響 (2009)
  • Author(s): 湯原 弘喜,一田 綾,大谷 里奈,前田 美季,淡路 水須,高橋 直子,内海 みよ子,有田 幹雄,牟礼 佳苗
  • Journal Title: 関西臨床スポーツ医科学研究会誌 Volume: 18 Pages: 45-6
  • Peer Reviewed
• [Journal Article] Eradication of Helicobacter pylori prevents cancer development in subjects with mild gastric atrophy identified by serum pepsinogen levels (2009)
  • Author(s): Yanaoka, K.
  • Journal Title: International Journal of Cancer 125 Pages: 2697-2703
  • Peer Reviewed
• [Journal Article] 高齢者におけるメンタルストレスへの反応性と運動習慣との関連 (2009)
  • Author(s): 池田敬子
  • Journal Title: 日本循環器予防学会誌 44 Pages: 141-151
  • Peer Reviewed
• [Presentation] MTHFR 遺伝子ヘテロ型のメタボリックシンドローム・動脈硬化指標への影響 (2013)
  • Author(s): 牟礼 佳苗、橋本麿和、服部園美、岩原昭 彦 、有田幹 雄、竹下達 也
  • Organizer: 第12回分子予防環境医学研究会
  • Place of Presentation: つくば市
  • Year and Date: 2013-02-02
• [Presentation] 職域男性におけるコーヒー摂取量および飲酒量と血清総アディポネクチン値との関連性 (2012)
  • Author(s): 前田真也, ら
  • Organizer: 第84回日本産業衛生学会
  • Place of Presentation: 東京都
  • Year and Date: 2012-05-19
• [Presentation] 紫外線誘発細胞ダメージにおける茶カテキンの影響評価 (2012)
  • Author(s): 牟礼 佳苗、竹下達也
  • Organizer: 第82回日本衛生学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2012-03-25
• [Presentation] 紫外線誘発ダメージにおける茶カテキンの影響評価 (2012)
  • Author(s): 牟礼佳苗, ら
  • Organizer: 第82回日本衛生学会
  • Place of Presentation: 京都市
  • Year and Date: 2012-03-25
• [Presentation] 職域男性におけるコーヒー摂取量および飲酒量と血清総アディポネクチン値との関連性 (2011)
  • Author(s): 前田 真也、牟礼佳苗、竹下達也、麦谷耕一、岩根幹能、木下藤寿、茂原治
  • Organizer: 第84回日本産業衛生学会
  • Place of Presentation: 東京都
  • Year and Date: 2011-05-19
• [Presentation] コーヒー摂取等の生活習慣とメタボリックシンドロームとの関連性について (2011)
  • Author(s): 牟礼 佳苗、前田真也、麦谷耕一、岩根幹能、木下藤寿、茂原治、竹下達也
  • Organizer: 第81回日本衛生学会総会
  • Place of Presentation: 東京都
  • Year and Date: 2011-03-26
• [Presentation] コーヒー摂取とメタボリックシンドローム関連健康指標 (2011)
  • Author(s): 牟礼 佳苗、前田真也、麦谷耕一、岩根幹能、木下藤寿、茂原治、竹下達也
  • Organizer: 第6回果実酒・果実飲料と健康に関する研究会
  • Place of Presentation: 和歌山県日高郡
  • Year and Date: 2011-02-11
• [Presentation] 肥満関連遺伝子多型とメタボリックシンドロームとの関連性 (2011)
  • Author(s): 牟礼 佳苗、前田真也、麦谷耕一、岩根幹能、木下藤寿、茂原治、竹下達也
  • Organizer: 第10回分子予防環境医学研究会
  • Place of Presentation: 京都市
  • Year and Date: 2011-01-22
• [Presentation] 肥満関連遺伝子多型とメタボリックシンドロームとの関連性 (2011)
  • Author(s): 牟礼佳苗
  • Organizer: 分子環境予防医学研究会大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-01-22
• [Presentation] 植物由来物質による紫外線誘発細胞障害抑制効果について (2010)
  • Author(s): 牟礼佳苗
  • Organizer: 第22回環境システム計測制御学会
  • Place of Presentation: 草津市
  • Year and Date: 2010-10-29
• [Presentation] 植物由来カロテノイドおよびポリフェノールの紫外線誘発ダメージ抑制効果について (2010)
  • Author(s): 牟礼 佳苗、竹下達也
  • Organizer: 第21回 環境システム計測制御学会
  • Place of Presentation: 草津市
  • Year and Date: 2010-10-28
• [Presentation] 職域男性における飲酒量と血清総アディポネクチン値との関連性の解析 (2010)
  • Author(s): 前田 真也、牟礼佳苗、竹下達也、渡辺実香、向林知津、麦谷耕一、渡辺豊、岩根幹能、木下藤寿、湯川修也、茂原治
  • Organizer: 第83回日本産業衛生学会
  • Place of Presentation: 福井市
  • Year and Date: 2010-05-27
• [Presentation] コーヒー摂取とメタボリックシンドロームの関連性について (2010)
  • Author(s): 牟礼 佳苗、前田真也、麦谷耕一、岩根幹能、木下藤寿、茂原治、竹下達也
  • Organizer: 第80回日本衛生学会総会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-05-10
• [Presentation] 職域男性におけるβ3 アドレナリン受容体遺伝子多型別の内臓脂肪面積、運動習慣と肝機能検査値との関連性についての解析 (2009)
  • Author(s): 前田 真也、牟礼佳苗、竹下達也、麦谷耕一、岩根幹能、木下藤寿、茂原治
  • Organizer: 第82回日本産業衛生学会
  • Place of Presentation: 北九州市
  • Year and Date: 2009-05-21
• [Presentation] 職域男性におけるβ3アドレナリン受容体遺伝子多型別の内臓脂肪面積、運動習慣と肝機能検査値との関連性についての解析 (2009)
  • Author(s): 前田真也
  • Organizer: 第82回日本産業衛生学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-05-21
• [Presentation] トマト由来リコペンの紫外線誘発ダメージ抑制効果 (2009)
  • Author(s): 牟礼 佳苗、竹下達也
  • Organizer: 第79回日本衛生学会
  • Place of Presentation: 東京都
  • Year and Date: 2009-03-31