Investigation of brain from infants with child abuse regarding to morphological change and epigenetic change such as DNA methylation.
Project/Area Number |
21659173
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Legal medicine
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Research Institution | Gunma University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Tamiko 群馬大学, 大学院・医学系研究科, 助教 (40008561)
SANO Rie 群馬大学, 大学院・医学系研究科, 助教 (70455955)
AMANUMA Makoto 群馬大学, 医学部, 准教授 (10212565)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥2,640,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2009: ¥800,000 (Direct Cost: ¥800,000)
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Keywords | 子どもの虐待 / 脳 / 形態学的観察 / DNAメチル化 / 脳CTイメージ / エピジェネティクス / 虐待 / Pyrosequencing / Pyrosequencing法 |
Research Abstract |
The purpose of this study is to investigate brain from infants with child abuse regarding to morphological change and epigenetic change such as DNA methylation. Morphological change of the brain was investigated using postmortem CT prior to autopsy, naked eye examination and microscopic examination of the brain tissue. As control, brains were used from cases of sudden or unexpected death. There was any significant morphological difference in brains between the affected person and controls. Next, to investigate DNA methylation in brains, preliminary experiments were carried out. We demonstrated that methylation specific PCR amplification could be carried out using DNA prepared from the paraffin. embedded brain tissues which had been fixed by formaldehyde. However, it was invalid to evaluate the extent of DNA methylation. Therefore, we need a method to discover a gene of which methylation is significantly associated with stress in infants. Thus, we are developing a method for enrichment of methylated DNA using precipitation of methylated DNA with methylation binding domain 1 protein, followed by sequencing. In future, it might be possible to identify a gene of which methylation is significantly associated with stress in infants by the method.
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Report
(4 results)
Research Products
(78 results)
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[Journal Article] Nonsynonymous single-nucleotide polymorphisms of the human apoptosis-related endonuclease-DNA fragmentation factor beta polypeptide, endonuclease G, and Flap endonuclease-1-genes show a lowdegree of genetic heterogeneity2012
Author(s)
Takeshita H, Fujihara J, Ueki, M, Iida R, Koda Y, Soejima M, Yuasa I, Kato H, Nakajima T, Kominato Y, Yasuda T
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Journal Title
DNA Cell Biol
Volume: 31
Pages: 36-42
Related Report
Peer Reviewed
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[Journal Article] Identification of the Brackish Water Clam Corbicula Japonica(Japanese Name, Yamato-Shijimi)and Specification of the Growing District by Polymerase Chain Reaction(PCR)-Based Analysis of Mitochondrial DNA2011
Author(s)
Fujihara J, Yasuda T, Ueki M, Fujita Y, Nakamura M, Oshiumi C, Hosozawa T, Nabika T, Harada Y, Kobayashi T, Nakajima T, Takeshita H
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Journal Title
Environmental Forensics
Volume: 12
Pages: 156-161
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Peer Reviewed
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[Journal Article] A case of fatal drug intoxication showing a high-density duodenal content by postmortem computed tomography2011
Author(s)
Sano R, Takahashi K, Kominato Y, Araki T, Yamamoto K, Takei H, Otake H, Awata S, Akuzawa H, Tago Y, Aoki H
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Journal Title
Legal Medicine
Volume: 13
Pages: 39-40
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Peer Reviewed
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[Journal Article] Identification of the brackish water clam Corbicula japonica (Japanese name, Yamato-shijimi) and pecification of the growing district by PCR-based analysis of mitochondrial DNA2011
Author(s)
Fujihara, J, Yasuda, T, Ueki M, Fujita Y, Nakamura M, Oshiumi C, Hosozawa T, Nabika T, Harada Y, Kobayashi T, Nakajima T, Takeshita H
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Journal Title
Environmental Forensics Journal
Volume: 9
Issue: 2
Pages: 25-31
DOI
Related Report
Peer Reviewed
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[Journal Article] A case of fatal drug intoxication showing a high-density duodenal content by postmortem computed tomography.2011
Author(s)
Sano R, Takahashi K, Kominato Y, Araki T, Yamamoto K, Takei H, Otake H, Awata S, Akuzawa H, Tago Y, Aoki H
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Journal Title
Leg Med (Tokyo).
Volume: 13
Pages: 39-40
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[Journal Article] 低酸素による核酸分解酵素DNASEI遺伝子の転写調節2010
Author(s)
佐野利恵, 中島たみ子, 小湊慶彦, 加藤恵理子, 高士祐一, 新田みなみ, 山根庸弘, 藤原純子, 竹下治男, 植木美鈴, 安田年博
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Journal Title
DNA多型
Volume: 18
Pages: 278-282
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Peer Reviewed
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[Journal Article] Development of a sensitive enzyme-linked immunosorbent assay for measurement of DNase I in human serum2009
Author(s)
Nakajima T, Takagi R, Tajima Y, Makita C, Kominato Y, Kuribara J, Ohshima S, Tada H, Tsurugaya H, Kobayashi Y, Takeshita H, Kawai Y, Yasuda T
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Journal Title
Clinica Chimica Acta
Volume: 403
Pages: 219-222
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[Journal Article] Caucasian-specific allele in non-synonymous single nucleotide polymorphisms of the gene encoding deoxyribonuclease I-like 3, potentially relevant to autoimmunity, produces an inactive enzyme2009
Author(s)
Ueki M, Takeshita H, Fujihara J, Iida R, Yuasa I, Kato H, Panduro A, Nakajima T, Kominato Y, Yasuda T
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Journal Title
Clinica Chimica Acta
Volume: 407
Pages: 20-24
Related Report
Peer Reviewed
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[Journal Article] Levels of serum deoxyribonuclease I activity on admission in patients with acute myocardial infarction can be useful in predicting left ventricular enlargement due to remodeling2009
Author(s)
Kuribara J, Tada H, Kawai Y, Kawaguchi R, Hoshizaki H, Arakawa K, Kitayama M, Kajinami K, Kurabayashi M, Oshima S, Taniguchi K, Kominato Y, Yasuda T
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Journal Title
Journal of Cardiology
Volume: 53
Pages: 196-203
NAID
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[Journal Article] 核酸分解酵素DNaseI遺伝子の転写調節機構の解明2009
Author(s)
高木利恵, 中島たみ子, 小湊慶彦, 加藤恵理子, 高士祐一, 新田みなみ, 山根庸弘, 藤原純子, 竹下治男, 植木美鈴, 安田年博
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Journal Title
DNA多型
Volume: 17
Pages: 177-179
NAID
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[Journal Article] Expression of ABO blood-group genes is dependent upon an erythroid cell-specific regulatory element, which is deleted in individuals with the B_m phenotype
Author(s)
Sano R, Nakajima T, Takahashi K, Kubo R, Kominato Y, Tsukada J, Takeshita H, Yasuda T, Ito K, Maruhashi T, Yokohama A, Isa K, Ogasawara K, Uchikawa M
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[Journal Article] Genetic variation of FUT2z in a Vietnamese population : identification of two novel Se enzyme. inactivating mutations
Author(s)
Soejima M, Fujimoto R, Agusa T, Iwata H, Fujihara J, Takeshita H, Minh TB, Trang PT, Viet PH, Nakajima T, Yoshimoto J, Tanabe S, Koda Y
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Journal Title
Transfusion
Volume: (In press)
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[Journal Article] Expression of ABO blood-group genes is dependent upon an erythroid cell-specific regulatory element, which is deleted in individuals with the B_m phenotype
Author(s)
Sano R, Nakajima T, Takahashi K, Kubo R, Kominato Y, Tsukada J, Takeshita H, Yasuda T, Ito K, Maruhashi T, Yokohama A, Isa K, Ogasawara K, Uchikawa M
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