Analysis ofchronic viral hepaptis by the large-scale next generation sequeincing
| Project/Area Number |
21659186
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
ENOMOTO Nobuyuki 山梨大学, 大学院・医学工学総合研究部, 教授 (20251530)
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| Co-Investigator(Kenkyū-buntansha) |
MAEKAWA Shinya 山梨大学, 大学院・医学工学総合研究部, 講師 (70397298)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2009: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | HCV / HBV / 次世代シークエンス / deep sequencing / シークエンス |
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| Research Abstract |
We developed the analytical method of rapid and large sccale sequenging of hepatitis virus genome in the patients with the next generation sequencer Roche Genome Sequencer Junior(GSJ), and revealed the dynamic changes of viral quasispcies constitution during the treatment and disease progression by the analysis of HCV core, NS3, NS5A genes and HBV pre S gene through deep sequencing.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Correlation between Pretreatment Viral Sequences and the Emergence of Lamivudine Resistance in Hepatitis B Virus Infection.2012
Author(s)
Sueki R, Maekawa S, Miura M, Kadokura M, Komase K, Shindo H, Kanayama A, Ohmori T, Shindo K, Amemiya F, Nakayama Y, Uetake T, Inoue T, Sakamoto M, Enomoto N.
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Journal Title
J Med Virol
Volume: (in press)
Related Report
Peer Reviewed
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[Journal Article] Pre-treatment prediction of response to pegylated-interferon plus ribavirin forchronic hepatitis C using genetic polymorphism in IL28B and viral factors.2011
Author(s)
Kurosaki M, Tanaka Y, Nishida N, SakamotoN, Enomoto N, Honda M, Sugiyama M, Matsuura K, Sugauchi F, Asahina Y, NakagawaM, Watanabe M, Sakamoto M, Maekawa S, SakaiA, Kaneko S, Ito K, Masaki N, Tokunaga K, Izumi N, Mizokami M.
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Journal Title
J Hepatol
Volume: 54(3)
Pages: 439-48
Related Report
Peer Reviewed
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[Journal Article] Analysis of the complete open reading frame of hepatitis C virus in genotype 2ainfection reveals critical sites influencing the response to peginterferon andribavirin therapy.2011
Author(s)
Kadokura M, Maekawa S, Sueki R, Miura M, Komase K, Shindo H, Amemiya F, Uetake T, Inoue T, Sakamoto M, Nakagawa M, SakamotoN, Watanabe M, Enomoto N.
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Journal Title
Hepatol Int
Volume: 5(3)
Pages: 789-99
Related Report
Peer Reviewed
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[Journal Article] Relationship between polymorphisms of theinosine triphosphatase geneand anaemia or outcome after treatment with pegylated interferon and ribavirin.2011
Author(s)
Kurosaki M, Tanaka Y, Tanaka K, Suzuki Y, Hoshioka Y, Tamaki N, Kato T, Yasui Y, Hosokawa T, Ueda K, Tsuchiya K, Kuzuya T, Nakanishi H, Itakura J, Takahashi Y, Asahina Y, Matsuura K, Sugauchi F, Enomoto N, Nishida N, Tokunaga K, Mizokami M, Izumi N.
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Journal Title
Antivir Ther
Volume: 16(5)
Pages: 685-94
Related Report
Peer Reviewed
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[Journal Article] Analysis of the complete open reading frame of genotype 2b hepatitis C virus inassociation with the response to peginterferon and ribavirin therapy.2011
Author(s)
Kadokura M, Maekawa S, Sueki R, Miura M, Komase K, Shindo H, Amemiya F, UetakeT, Inoue T, Sakamoto M, Nakagawa M, SakamotoN, Watanabe M, Enomoto N.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Characterization of naturally occurring proteaseinhibitor-resistance mutations in genotype 1b hepatitis C virus patients.2011
Author(s)
Shindo H, Maekawa S, Komase K, Sueki R, Miura M, Kadokura M, Shindo K, Amemiya F, Kitamura T, Nakayama Y, Inoue T, Sakamoto M, Okada SI, Asahina Y, Izumi N, Honda M, Kaneko S, Enomoto N.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Analysis of viral amino acids sequences and the IL28B SNP in fluencing the development of hepatocellular carcinoma in chronic hepatitis C.2011
Author(s)
Miura M, Maekawa S, Kadokura M, Sueki R, Komase K, Shindo H, Ohmori T, Kanayama A, Shindo K, Amemiya F, Nakayama Y, Kitamura T, Uetake T, Inoue T, Sakamoto M, Okada S, Enomoto N
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] A predictive model of response to peginterferon ribavirin in chronic hepatitis C using classification andregression tree analysis.2010
Author(s)
Kurosaki M, Matsunaga K, Hirayama I, Tanaka T, Sato M, Yasui Y, Tamaki N, Hosokawa T, Ueda K, Tsuchiya K, Nakanishi H, Ikeda H, Itakura J, Takahashi Y, Asahina Y, Higaki M, Enomoto N, Izumi N.
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Journal Title
Hepatol Res
Volume: 40(3)
Pages: 251-60
Related Report
Peer Reviewed
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[Journal Article] Mutations in the interferon sensitivity determining region and virological response to combination therapy with pegylated-interferon alpha 2b plus ribavirin in patients with chronic hepatitis C-1b infection.2010
Author(s)
Nakagawa M, Sakamoto N, Ueyama M, Mogushi K, Nagaie S, Itsui Y, Azuma S, Kakinuma S, Tanaka H, Enomoto N, WatanabeM.
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Journal Title
J Gastroenterol
Volume: 45(6)
Pages: 656-65
NAID
Related Report
Peer Reviewed
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[Journal Article] ITPA gene variant protects against anemia induced by pegylated interferon-αand ribavirin therapy for Japanese patients with chronic hepatitis C.2010
Author(s)
Sakamoto N, Tanaka Y, Nakagawa M, Yatsuhashi H, Nishiguchi S, Enomoto N, AzumaS, Nishimura-Sakurai Y, Kakinuma S, Nishida N, Tokunaga K, Honda M, Ito K, Mizokami M, Watanabe M.
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Journal Title
Hepatol Res
Volume: 40(11)
Pages: 1063-1071
Related Report
Peer Reviewed
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