| Project/Area Number |
21659210
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Juntendo University |
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Principal Investigator |
SEYAMA Kuniaki 順天堂大学, 医学部, 先任准教授 (10226681)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,040,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2009: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | リンパ脈管筋腫症 / LAM細胞 / リンパ管内皮細胞 / 初代培養 / 腫瘍幹細胞 / 癌抑制遺伝子 / 結節性硬化症 / TSC2 / モデル動物 |
|---|
| Research Abstract |
Isolation of LAM stem cells, LAM cells, LAM-associated lymphatic endothelial cells(LCC) was tried from LAM-affected lung tissues LAM cell cluster(LCC). Establishing this technique will be expected to contribute to the in-depth analysis of interaction among LAM stem cell, LAM cells and LCC with three-dimensional reconstitution in vitro culture system and to the possible application for the development of LAM mouse model. However, we experienced difficulty to prepare cell suspension with an acceptable level of cell viability from LAM-affected lung tissues. At the 3rd year of our project, we adopted the method published by the other group in which FACS sorting was utilized to fractionate alveolar epithelial cells, vascular endothelial cells, LCC, and mesenchymal cells. LAM sand its application for the establishment of mouse LAM model. By applying this method to two LAM-associated lung tissues, we could reproducibly prepare cell suspension with high cell viability and successfully fractionate cell fraction which was likely to be unique to LAM-affected lung tissues.
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