| Project/Area Number |
21659230
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Metabolomics
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KURISAKI Hironori 九州大学, 医学研究院, 助教 (70403962)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,270,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2009: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | 自己免疫調節(AIRE)遺伝子 / B細胞 / 自己免疫膵炎 / 1型糖尿病 / 自己免疫調節遺伝子(Aire) / Bリンパ球 / 膵島炎 / 膵臓炎 / 抗原提示 |
|---|
| Research Abstract |
Since we have already reported that B cells are essential to the progression of insulitis and diabetes in NOD mice(Int Immunol 1997), the exact mechanisms and the role of B cells and aire gene have been studied in Balb/c mice as well as NOD mice. It was found that aire gene was highly expressed in B cells irrespective of the mouse strains. In addition, we found the 64kd novel autoantibody in aire gene knockout Balb/c mice with autoimmune pancreatitis.
On the other hand, in human B cells, accumulating data have shown that expressed AIRE proteins in B cells had two different sizes, indicating the expressed AIRE proteins in B cells might have multiple functions, possibely due to different splicing patterns. We further intend to analyze the role of AIRE protein s expressed in B cells using immunoprocipitation assay and also chromatin-immunoprrecipitation method to identify the interacting proteins and/or genetic regions which may play transcriptional regulation of autoimmune-associated target genes.
|
