Development of novel therapeutic strategies that targets intestinal epithelial endocrine cells
| Project/Area Number |
21659232
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Endocrinology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAWADA Makoto 名古屋大学, 環境医学研究所, 教授 (10187297)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,140,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2009: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | 内分泌学 / 糖尿病 / 発生 / 分化 / インクレチン / GLP-1 / バイオイメージング / L-細胞 / 創薬 |
|---|
| Research Abstract |
Glucagon-like peptide-1(GLP-1), which stimulates insulin secretion from islet.-cells, is produced in enteroendocrine L cells. As L cells are distributed in a scattered manner in intestinal epithelium, selective isolation of L cells has been difficult. Elucidation of mechanisms regulating differentiation and proliferation of L cells, and regulatory mechanism of GLP-1 secretion, should contribute to development of novel drugs to treat diabetes, which regulate production of endogenous GLP-1. In the present study, we developed a novel gene-modified animal model, in which green fluorescent protein is expressed in an L cell specific manner. We succeeded in selective isolation of L cells and identification of genes expressed in an L cell specific manner. Functional analyses of such genes should lead to development of novel drugs to treat diabetes mellitus.
|
Report
(4 results)
Research Products
(30 results)
-
[Journal Article] Remodeling of hepatic metabolism and hyper aminoacidemia in mice deficient in proglucagon-derived peptides2012
Author(s)
Watanabe, C., Seino, Y., Miyahira, H., Yamamoto, M., Fukami, A., Ozaki, N., Takagishi, Y., Sato, J., Fukuwatari, T., Shibata, K., Oiso, Y., Murata, Y., and Hayashi, Y.
-
Journal Title
Diabetes
Volume: 61
Pages: 74-84
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] Mice deficient for glucagon gene-derived peptides display normoglycemia and hyperplasia of islet a-cells but not of intestinal L-cells2009
Author(s)
Hayashi, Y., Yamamoto, M., Mizoguchi, H., Watanabe, C., Ito, R., Yamamoto, S., Sun, X. Y., and Murata, Y.
-
Journal Title
Mol. Endocrinol
Volume: 23
Pages: 1990-1999
Related Report
Peer Reviewed
-
-
-
-
-
-
-
[Presentation] Evaluation of b-cell function in proglucagon-EGFP knock-in mice2011
Author(s)
Fukami, A., Seino, Y., Ozaki, N., akamoto, E., Kato, J., Tsunekawa, S., Hayashi, Y., Murata, Y., Oiso, Y.
Organizer
47^<th> EASD annual meeting
Place of Presentation
Lisbon, Portugal
Year and Date
2011-09-14
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
