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Systematic survey for biologically active peptides in human pheochromocytoma tissue.

Research Project

Project/Area Number 21659234
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Endocrinology
Research InstitutionUniversity of Miyazaki

Principal Investigator

KITAMURA Kazuo  宮崎大学, 医学部, 教授 (50204912)

Co-Investigator(Kenkyū-buntansha) KUWASAKO Kenji  宮崎大学, フロンティア科学実験総合センター, 准教授 (20381098)
NAGATA Sayaka  宮崎大学, 医学部, 助教 (00452920)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,170,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2009: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords褐色細胞腫 / 受容体 / 新規生理活性ペプチド / cAMP / アミド化 / 血管作動性物 / アンジオテンシンン / ラジオイムノアッセイ / アンジオテンシン / Ca^<++> / 生理活性ペプチド / C末端アミド化 / 血管作動性物質 / 血管作動性質
Research Abstract

We have performed the systematic survey for biologically active peptides in human pheochromocytoma tissue, using assay systems which we had originally established. We have purified and determined more than 20 peptides from human human pheochromocytoma tissue. We chemically synthesized the novel peptide and are now investigating its function.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (28 results)

All 2012 2011 2010 2009

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (8 results) Book (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Plasma and tissue concentrations of proangiotensin-12 in rats treated with inhibitors of the renin-angiotensin system2012

    • Author(s)
      Nagata S, Kuwasako K, Kitamura K
    • Journal Title

      Hypertens Res 2012

      Volume: 35 Pages: 234-238

    • NAID

      10030643913

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of proangiotensin-12 infused continuously over 14days in conscious rats2012

    • Author(s)
      Kuwasako K, Nagata S, Kitamura K
    • Journal Title

      Eur J Pharmacol 2012

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasma and tissue concentrations of proangiotensin-12 in rats treated with inhibitors of the renin-angiotensin system2012

    • Author(s)
      Nagata S, Kuwasako K, Kitamura K, et al
    • Journal Title

      Hypertens Res

      Volume: 35 Pages: 234-238

    • NAID

      10030643913

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of proangiotensin-12 infused continuously over 14days in conscious rats2012

    • Author(s)
      Kuwasako K, Nagata S, Kitamura K, et al
    • Journal Title

      Eur J Pharmacol

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shared and separate functions of the RAMP-based adrenomedullin receptors2011

    • Author(s)
      Kuwasako K, Nagata S, Kitamura K
    • Journal Title

      Peptides 2011

      Volume: 32 Pages: 1540-1550

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Shared and separate functions of the RAMP-based adrenomedullin receptors2011

    • Author(s)
      Kuwasako K, Nagata S, Kitamura K, et al
    • Journal Title

      Peptides

      Volume: 32 Pages: 1540-1550

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Function of the cytoplasmic tail of human calcitonin receptor-like receptor in complex with receptor activity-modifying protein 22010

    • Author(s)
      Kuwasako K, Kitamura K, Nagata S, Hikosaka T, Kato J
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 392 Pages: 380-385

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasma and tissue levels of proangiotensin-12 and components of the renin-angiotensin system(RAS) following low-or high-salt feeding in rats2010

    • Author(s)
      Nagata S, Kato J, Kuwasako K, Kitamura K
    • Journal Title

      Peptides 2010

      Volume: 31 Pages: 889-892

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure-function analysis of helix 8 of human calcitonin receptor-like receptor within the adrenomedullin 1 receptor2010

    • Author(s)
      Kuwasako K., Kitamura K, Nagata S, Hikosaka T, Kato J
    • Journal Title

      Peptides 2010

      Volume: 32 Pages: 144-149

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Function of the cytoplasmic tail of human calcitonin receptor-like receptor in complex with receptor activity-modifying protein 2.2010

    • Author(s)
      Kuwasako K, Kitamura K, Nagata S, Hikosaka T, Kato J.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 392 Pages: 380-385

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma and tissue levels of proangiotensin-12 and components of the renin-angiotensin system (RAS) following low- or high-salt feeding in rats.2010

    • Author(s)
      Nagata S, Kato J, Kuwasako K, Kitamura K.
    • Journal Title

      Peptides

      Volume: 31 Pages: 889-892

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure-function analysis of helix 8 of human calcitonin receptor-like receptor within the adrenomedullin 1 receptor.2010

    • Author(s)
      Kuwasako K., Kitamura K, Nagata S, Hikosaka T, Kato J.
    • Journal Title

      Peptides

      Volume: 32 Pages: 144-149

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Function of the cytoplasmic tail of human calcitonin receptor-like receptor in complex with receptor activity-modifying protein 22010

    • Author(s)
      Kuwasako K, Kitamura K, et al.
    • Journal Title

      Biochem Biophys Res Commun 392

      Pages: 380-385

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma and tissue levels of proangiotensin-12 and components of the ren in-angiotensin system (RAS) following low- or high-salt feeding in rats2010

    • Author(s)
      Nagata S, Kato J, et al.
    • Journal Title

      Peptides 31

      Pages: 889-892

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential regulation of angiotensin-(1-12) in plasma and cardiac tissue in response to bilateral nephrectomy2009

    • Author(s)
      Ferrario CM, Varagic J
    • Journal Title

      Am J Physiol Heart Circ Physiol

      Volume: 296

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential regulation of angiotensin-(1-12) in plasma and cardiac tissue in response to bilateral nephrectomy2009

    • Author(s)
      Ferrario CM, Varagic J, et al.
    • Journal Title

      Am J Physiol Heart Circ Physiol 296

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Beneficial Biological Effects of Adrenomedullin and Possibitity of Clinical Application2012

    • Author(s)
      Kitamura K
    • Organizer
      BIT's 5th Anniversary of Protein and Peptide Conference(PepCon-2012)
    • Place of Presentation
      Beijing, China
    • Year and Date
      2012-03-23
    • Related Report
      2011 Final Research Report
  • [Presentation] Beneficial Biological Effects of Adrenomedullin and Possibitity of Clinical Application2012

    • Author(s)
      Kitamura K.
    • Organizer
      BIT'S 5^<th> Anniversary of Protein and Peptide Conference (PepCon-2012)
    • Place of Presentation
      Beijing, China(招待講演)
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Interaction between receptor activity-modifying proteins(RAMPs) and G-protein-coupled receptors and the function of the C-terminal tail of calcitonin receptor-like receptor in complex with RAMP22010

    • Author(s)
      Kuwasako K, Kitamura K, Nagata S, Hikosaka T, Kato J
    • Organizer
      7th International Symposia on the CGRP Family ; CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin
    • Place of Presentation
      Queenstown, New Zealand
    • Year and Date
      2010-08-31
    • Related Report
      2011 Final Research Report
  • [Presentation] Interaction between receptor activity-modifying proteins (RAMPs) and G-protein-coupled receptors and the function of the C-terminal tail of calcitonin receptor-like receptor in complex with RAMP2.2010

    • Author(s)
      Kuwasako K, Kitamura K, Nagata S, Hikosaka T, Kato J
    • Organizer
      7^<th> International Symposia on the CGRP Family ; CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin
    • Place of Presentation
      Queenstown, New Zealand
    • Year and Date
      2010-08-31
    • Related Report
      2010 Annual Research Report
  • [Presentation] Adrenomedullin treatment reduces intestinal inflammation in mice administered dextran sulphate sodium2010

    • Author(s)
      Kitamura K, Ashizuka S
    • Organizer
      14th International Congress of Endocrinology(ICE2010)
    • Place of Presentation
      京都
    • Year and Date
      2010-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Possibility for clinical application of adrenomedullin and related peptides2010

    • Author(s)
      Kitamura K
    • Organizer
      14th International Congress of Endocrinology(ICE2010)
    • Place of Presentation
      京都
    • Year and Date
      2010-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Possibility for clinical application of adrenomedullin and related peptides2010

    • Author(s)
      Kitamura K.
    • Organizer
      14^<th> International Congress of Endocrinology (ICE2010)
    • Place of Presentation
      京都
    • Related Report
      2009 Annual Research Report
  • [Presentation] Adrenomedullin treatment reduces intestinal inflammation in mice administered dextran sulphate sodium2010

    • Author(s)
      Kitamura K, Ashizuka S, et.al.
    • Organizer
      14^<th> International Congress of Endocrinology (ICE2010)
    • Place of Presentation
      京都
    • Related Report
      2009 Annual Research Report
  • [Book] マクロファージ・血管構築細胞群2011

    • Author(s)
      鶴田敏博、畠山金太
    • Total Pages
      5
    • Publisher
      日本臨床社
    • Related Report
      2011 Final Research Report
  • [Book] 炎症と動脈硬化.マクロファージ・血管構築細胞群2011

    • Author(s)
      鶴田敏博、畠山金太, ほか
    • Total Pages
      5
    • Publisher
      日本臨床社
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] マトリックスメタロプロテアーゼ活性抑制組成物2010

    • Inventor(s)
      鶴田敏博、北村和雄
    • Industrial Property Rights Holder
      国立大学法人宮崎大学
    • Industrial Property Number
      2010-187078
    • Filing Date
      2010-08-24
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] マトリックスメタロプロテアーゼ活性抑制組成物2010

    • Inventor(s)
      鶴田敏博
    • Industrial Property Rights Holder
      宮崎大学
    • Industrial Property Number
      2010-187078
    • Filing Date
      2010-08-24
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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