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Human arterial chromosomes for new hemophilia therapy

Research Project

Project/Area Number 21659238
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionSojo University (2011)
Tottori University (2009-2010)

Principal Investigator

TAKETA Hiroyuki  崇城大学, 生物生命学部, 教授 (60222105)

Co-Investigator(Renkei-kenkyūsha) KUROSAKI Hajime  鳥取大学, 大学院・医学系研究科, 助教 (70464295)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywords分子血栓止血学 / 遺伝子治療 / 組換えタンパク / 血友病 / 遺伝子工学 / 細胞工学 / 移植・再生医療 / 遺伝子 / ゲノム / 組換えタンパク質 / 人工染色体 / iPS細胞 / ヒト人工染色体 / 血小板 / 血液凝固 / 第VIII因子 / 凝固因子 / 血液凝固因子 / 凝固第VIII因子
Research Abstract

Human artificial chromosome(HAC) vectors show considerable promise for gene therapy applications as they have the capacity to deliver an extremely large genetic region and are stably maintained independent of the host chromosome, thus diminishing risks of insertional mutagenesis. In this study, we have developed HAC vectors for gene therapy of hemophilia.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (52 results)

All 2012 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 8 results) Presentation (38 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Snake venom metalloproteinases : structure, function and relevance to the mammalian ADAM/ADAMTS family proteins2012

    • Author(s)
      Takeda S, Takeya H, Iwanaga S
    • Journal Title

      Biochim Biophys Acta

      Volume: 1824 Issue: 1 Pages: 164-76

    • DOI

      10.1016/j.bbapap.2011.04.009

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sphingomyelin synthase 1-generated sphingomyelin plays an important role in transferrin trafficking and cell proliferation2011

    • Author(s)
      Shakor AB, Taniguchi M, Kitatani K, Hashimoto M, Asano S, Hayashi A, Nomura K, Bielawski J, Bielawska A, Watanabe K, Kobayashi T, Igarashi Y, Umehara H, Takeya H, Okazaki T.
    • Journal Title

      J Biol Chem

      Volume: 286 Pages: 36053-62

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Integration-free and stable expression of FVIII using a human artificial chromosome2011

    • Author(s)
      Kurosaki H, et.al.
    • Journal Title

      Journal of Human Genetics

      Volume: 56 Issue: 10 Pages: 727-733

    • DOI

      10.1038/jhg.2011.88

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of dibutyryl cyclic adenosine monophosphate on the gene expression of plasminogen activator inhibitor-1 and tissue factor in adipocytes2011

    • Author(s)
      Taniguchi M, Ono N, Hayashi A, Yakura Y, Takeya H.
    • Journal Title

      Thromb Res

      Volume: 128 Pages: 375-80

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of dibutyryl cyclic adenosine monophosphate on the gene expression of piasminogen activator inhibitor-1 and tissue factor in adipocytes2011

    • Author(s)
      Makoto Taniguchi
    • Journal Title

      Thromb Res

      Volume: 128 Issue: 4 Pages: 375-380

    • DOI

      10.1016/j.thromres.2011.03.005

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sphingomyelin synthase 1-generated sphingomyelin plays an important role in transferrin trafficking and cell proliferation2011

    • Author(s)
      Abo Bakr Abdel Shakor
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Issue: 41 Pages: 36053-36062

    • DOI

      10.1074/jbc.m111.228593

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 変貌するDICの診断と基準凝固線溶系:凝固系の最近の進歩2011

    • Author(s)
      鈴木宏治
    • Journal Title

      Thrombosis Medicine

      Volume: 56 Pages: 727-733

    • Related Report
      2011 Annual Research Report
  • [Journal Article] 血栓症に関するQ&A:流血中の組織因子は血栓症の病態にどの程度関与しているのですか?2011

    • Author(s)
      武谷浩之
    • Journal Title

      血栓と循環

      Volume: 19 Pages: 53-55

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 生物毒:血液毒-血栓・抗血栓性因子および出血因子2009

    • Author(s)
      武谷浩之
    • Journal Title

      生物の科学 遺伝 Vol.63

      Pages: 14-18

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 細胞による凝固制御2009

    • Author(s)
      武谷浩之
    • Journal Title

      International Review of Thrombosis Vol.4

      Pages: 22-28

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Effect of dibutyryl cyclic adenosine monophosphate on the gene expression of plasminogen activator inhibitor-1 and tissue factor in adipocytes

    • Author(s)
      Makoto Taniguchi
    • Journal Title

      Thromb Res

      Volume: (掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Snake venom metalloproteinases : structure, function and relevance to the mammalian ADAM/ADAMTS family proteins

    • Author(s)
      Soichi Takeda
    • Journal Title

      Biochim Biophis Acta

      Volume: (掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 人工染色体ベクターを用いた新規血友病A遺伝子治療法の開発2012

    • Author(s)
      矢倉裕奈、黒崎創、石原千恵、香月康宏、土井健史、松下正、武谷浩之、押村光雄
    • Organizer
      第11回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2012-06-14
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト人工染色体を用いた血小板特異的FVIII発現iPS細胞の作製-新規治療法確立へ向けて2012

    • Author(s)
      矢倉裕奈、黒崎創、石原千恵、香月康宏、土井健史、武谷浩之、押村光雄
    • Organizer
      第34回日本血栓止血学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2012-06-09
    • Related Report
      2011 Final Research Report
  • [Presentation] Construction of animal models for diabetes using nematode C.elegans2011

    • Author(s)
      Eri Teshiba
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Sphingomyelin synthase 1 deletion causes thrombocytopenia due to increased apoptosis2011

    • Author(s)
      Akira Hayashi
    • Organizer
      第73回日本血液学会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2011-10-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト人工染色体とiPS細胞を用いた新規血友病遺伝子治療法2011

    • Author(s)
      矢倉裕奈、石原千恵、藤井昂洸、香月康宏、黒崎創、土井健史、小松則夫、押村光雄、武谷浩之
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト人工染色体とiPS細胞を用いた新規血友病遺伝子治療法2011

    • Author(s)
      矢倉裕奈
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Transcription factor NFAT but not NF-KB is essential for thrombin-induced tissue factor expression2011

    • Author(s)
      Yakura Y, Taniguchi M, Morimoto K, Hayashi A, Takeya H
    • Organizer
      XXIII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      京都
    • Year and Date
      2011-07-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Transcription factor NFAT but not NF-kB is essential for thrombin-induced tissue factor expression2011

    • Author(s)
      Yuwna Yakura
    • Organizer
      XXIII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2011-07-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Suppressive actions of fucoidan on lipid droplet formation and plasminogen activator inhibitor-1 expression in adipocytes2011

    • Author(s)
      Hayashi A, Yakura Y, Taniguchi M, Tomita A, Takeya H
    • Organizer
      XXIII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      京都
    • Year and Date
      2011-07-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Regulation of leukotriene production and plasimonen activator inhibitor-1 expression in adipose tissue2011

    • Author(s)
      Hayashi A, Taniguchi M, Yakura Y, Miyabara J, Yamamoto H, Kozutsumi Y, Okazaki T, Takeya H
    • Organizer
      XXIII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      京都
    • Year and Date
      2011-07-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Suppressive actions of fucoidan on lipid droplet formation and plasminogen activator inhibitor-1 expression in adipocytes2011

    • Author(s)
      Akira Hayashi
    • Organizer
      XXIII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2011-07-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Regulation of leukotriene production and plasimonen activator inhibitorl expression in adipose tissue2011

    • Author(s)
      Akira Hayashi
    • Organizer
      XXIII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2011-07-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Thrombocytopenia in mice lacking sphingomyelin synthase 12011

    • Author(s)
      Fujii, T, Hayashi A, Yakura Y, Taniguchi M, Watanabe K, Kitatani K, Okazaki T, Takeya H
    • Organizer
      XXIII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      京都
    • Year and Date
      2011-07-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Thrombocytopenia in mice lacking sphingomyelin synthase 12011

    • Author(s)
      Takahiro Fujii
    • Organizer
      XXIII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2011-07-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] スフィンゴシン1-リン酸によるMonocyte chemoattractant protein-1(MCP-1)の遺伝子発現誘導経路の解析2010

    • Author(s)
      武谷浩之
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      ポートアイランド(神戸)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Pathophysiology of thrombocytopenia in mice lacking sphingomyelin synthase 12010

    • Author(s)
      武谷浩之
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      ポートアイランド(神戸)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] セラミド依存的なATM活性化によるアポトーシス誘導2010

    • Author(s)
      岡崎俊郎
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      ポートアイランド(神戸)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] スフィンゴシン-1-リン酸(S1P)によるセラミド誘導性オートファジーの抑制2010

    • Author(s)
      岡崎俊郎
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      ポートアイランド(神戸)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] スフィンゴミエリンによる走化性運動の制御2010

    • Author(s)
      岡崎俊郎
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      ポートアイランド(神戸)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] トロンビンによる組織因子の遺伝子発現誘導における転写因子の解析2010

    • Author(s)
      武谷浩之
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      ポートアイランド(神戸)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 血栓形成の分子機構2010

    • Author(s)
      武谷浩之
    • Organizer
      心臓血管フォーラム2010
    • Place of Presentation
      モントレグラスミア(大阪)
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] スフィンゴミエリン合成酵素1-KOマウスの解析:血小板減少について2010

    • Author(s)
      武谷浩之
    • Organizer
      セラミド研究会第3回学術集会
    • Place of Presentation
      東大弥生講堂(東京)
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] スフィンゴミエリン合成酵素2欠損マウスにおけるTリンパ腫転移の亢進2010

    • Author(s)
      岡崎俊郎
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] スフィンゴミエリン合成酵素1-KOマウスの解析:血小板減少について2010

    • Author(s)
      林輝
    • Organizer
      第5回スフィンゴテラピー研究会
    • Place of Presentation
      米子市観光センター(米子)
    • Year and Date
      2010-07-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] スフィンゴシン-1-リン酸(S1P)によるmTOR活化を介したオートファジー抑制機構2010

    • Author(s)
      岡崎俊郎
    • Organizer
      第5回スフィンゴテラピー研究会
    • Place of Presentation
      米子市観光センター(米子)
    • Year and Date
      2010-07-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] セラミド依存的なATM活性化によるアポトーシス誘導2010

    • Author(s)
      岡崎俊郎
    • Organizer
      第5回スフィンゴテラピー研究会
    • Place of Presentation
      米子市観光センター(米子)
    • Year and Date
      2010-07-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] スフィンゴミエリンによる走化性運動の制御2010

    • Author(s)
      岡崎俊郎
    • Organizer
      第5回スフィンゴテラピー研究会
    • Place of Presentation
      米子市観光センター(米子)
    • Year and Date
      2010-07-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mice lacking sphingomyelin synthase 1 exhibit thrombocytopenia2010

    • Author(s)
      Hiroyuki Takeya
    • Organizer
      2010 Keystone Symposia : Bioactive lipids
    • Place of Presentation
      ウェスチン都(京都)
    • Year and Date
      2010-06-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] トロンビンによる組織因子発現誘導にNF-κBの活性化は必須ではない2010

    • Author(s)
      武谷浩之
    • Organizer
      第33回日本血栓止血学会学術集会
    • Place of Presentation
      城山観光ホテル(鹿児島)
    • Year and Date
      2010-04-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] スフィンゴミエリン合成酵素1の遺伝子欠損マウスは血小板数減少を来す2010

    • Author(s)
      武谷浩之
    • Organizer
      第33回日本血栓止血学会学術集会
    • Place of Presentation
      城山観光ホテル(鹿児島)
    • Year and Date
      2010-04-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] キチン類スポンジ止血材の開発2010

    • Author(s)
      黒住誠司
    • Organizer
      第33回日本血栓止血学会学術集会
    • Place of Presentation
      城山観光ホテル(鹿児島)
    • Year and Date
      2010-04-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Development of human artificial chromosome vectors for gene therapy of hemophilia A2009

    • Author(s)
      Yakura Y, Ishihara C, Hayashi A, Matsui T, Matsumoto S, Kurosaki H, Oshimura M, Doi T, Komatsu N, and Takeya H
    • Organizer
      XXIIst Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      Boston
    • Year and Date
      2009-07-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of human artificial chromosome vectors for gene therapy of hemophilia A2009

    • Author(s)
      Hiroyuki Takeya
    • Organizer
      XXII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      Boston, USA
    • Year and Date
      2009-07-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Covalently associated dimers and multimers of β2-glycoprotein I2009

    • Author(s)
      Hiroyuki Takeya
    • Organizer
      XXII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      Boston, USA
    • Year and Date
      2009-07-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] The role of cAMP in the gene expression of plasminogen activator inhibitor-1 and tissue factor in adipocytes2009

    • Author(s)
      Hiroyuki Takeya
    • Organizer
      XXII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      Boston, USA
    • Year and Date
      2009-07-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト人工染色体を用いた血小板特異的な第VIII因子発現系の確立2009

    • Author(s)
      武谷浩之
    • Organizer
      第32回日本血栓止血学会学術集会
    • Place of Presentation
      北九州国際会議場(小倉)
    • Year and Date
      2009-06-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト人工染色体を用いた血小板特異的な第VIII因子発現系の確立2009

    • Author(s)
      矢倉裕奈、石原千恵、林輝、松井俊樹、松本幸子、黒崎創、押村光雄、土井健史、小松則夫、武谷浩之
    • Organizer
      第32回日本血栓止血学会学術集会
    • Place of Presentation
      北九州
    • Year and Date
      2009-06-04
    • Related Report
      2011 Final Research Report
  • [Presentation] The ability of mature adipocytes to produce cysteinyl leukotrienes2009

    • Author(s)
      Hiroyuki Takeya
    • Organizer
      4^<th> International Conference on Phospholipase A2 and Lipid Madiators (PLM2009)
    • Place of Presentation
      National Center of Sciences (Tokyo)
    • Year and Date
      2009-05-27
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 線溶賦活剤2009

    • Inventor(s)
      武谷浩之
    • Industrial Property Rights Holder
      鳥取大学
    • Industrial Property Number
      2009-115734
    • Filing Date
      2009-05-12
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 線溶賦活剤2009

    • Inventor(s)
      武谷浩之
    • Industrial Property Rights Holder
      鳥取大学
    • Acquisition Date
      2009-12-25
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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