Development of transplantable self-regenerative liver tissue consisted of hepatocytes and stem cells
Project/Area Number |
21659307
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
General surgery
|
Research Institution | Nagasaki University |
Principal Investigator |
KANEMATSU Takashi 長崎大学, 大学院・医歯薬学総合研究科, 名誉教授 (40128004)
|
Co-Investigator(Kenkyū-buntansha) |
EGUCHI Susumu 長崎大学, 大学院・医歯薬学総合研究科, 教授 (80404218)
KAMOHARA Tukio 独立行政法人国立病院機構長崎医療センター, 臨床研究センター・外科治療研究室, 室長 (50325643)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2009: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
Keywords | 肝細胞 / 幹細胞 / 自己再生型 / 肝細胞移植 / 肝移植 / 可移植性組織片 / 細胞シート / 肝細胞シート |
Research Abstract |
We established the procedures of cultured hepatocyte sheet and fibroblast sheet in this study. Fibroblast sheet was transplantable to the abdominal cavity and showed excellent adaptation in vivo. We have discovered that fibroblast sheet enhanced tissue repair microscopically and induced accumulation of oval cell, a hepatic progenitor cell. We have not got an evidence of hepatic stem cells from fibroblast sheet itself, however, using recipient specific markers, accumulated cells were originated from recipient. Thus, we speculate that fibroblast sheet promote recipient stem cells thorough the improvement of injured recipient environment. In this study, we also have attempted that coculture system of hepatocyte and fibroblast sheet. Technically, it has been achieved, but, the cellular assembly remained mature tissue. Although further study is needed to obtain initial purposes, fibroblast sheet is expecting candidate for the transplantable tissue engineering which promotes stem cells.
|
Report
(4 results)
Research Products
(97 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Multicentric occurrence and spread of hepatocellular carcinoma in whole explanted end-stage liver2009
Author(s)
Hidaka M, Eguchi S, Okudaira S, Takatsuki M, Tokai H, Soyama A, Nagayoshi S, Mochizuki S, Hamasaki K, Tajima Y, Kanematsu T
-
Journal Title
Hepatology Research
Volume: 39(2)
Pages: 143-148
NAID
Related Report
-
[Journal Article] Acute Deterioration of Idiopathic Portal Hypertension Requiring Living Donor Liver Transplantation : A Case Report2009
Author(s)
Inokuma T, Eguchi S, Tomonaga T, Miyazaki K, Hamasaki K, Tokai H, Hidaka M, Yamanouchi K, Takatsuki M, Okudaira S, Tajima Y, Kanematsu T
-
Journal Title
Digestive Diseases and Sciences.
Volume: 54(7)
Pages: 1597-601
NAID
Related Report
-
-
-
[Journal Article] Multicentric occurrence and spread of hepatocellular carcinoma in whole explanted end-stage liver.2009
Author(s)
Hidaka M, Eguchi S, Okudaira S, Takatsuki M, Tokai H, Soyama A, Nagayoshi S, Mochizuki S, Hamasaki K, Tajima Y, Kanematsu T
-
Journal Title
Hepatology Research 39
Pages: 143-148
NAID
Related Report
Peer Reviewed
-
[Journal Article] Acute Deterioration of Idiopathic Portal Hypertension Requiring Living Donor Liver Transplantation : A Case Report.2009
Author(s)
Inokuma T, Eguchi S, Tomonaga T, Miyazaki K, Hamasaki K, Tokai H, Hidaka M, Yamanouchi K, Takatsuki M, Okudaira S, Tajima Y, Kanematsu T
-
Journal Title
Digestive Diseases and Sciences 54
Pages: 1597-1601
NAID
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] Hepatitis C virus kinetics during the first phase of pegylated interferon-α-2b with ribavirin therapy in patients with living donor liver transplantation.2009
Author(s)
Ichikawa T, Nakao K, Miyaaki H, Eguchi S, Takatuski M, Fujimito M, Akiyama M, Miuma S, Ozawa E, Shibata H, Takeshita S, Kanematsu T, Eguchi K
-
Journal Title
Hepatology Research 39
Pages: 856-864
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] Actual therapeutic efficacy of pre-transplant treatment on hepatocellular carcinoma and its impact on survival after salvage living donor liver transplantation.2009
Author(s)
Eguchi S, Hidaka M, Tomonaga T, Miyazaki K, Inokuma T, Takatsuki M, Okudaira S, Yamanouchi K, Miyaaki H, Ichikawa T, Tajima Y, Kanematsu T
-
Journal Title
Journal of Gastroenterology 44
Pages: 624-629
NAID
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] The significance of enzyme immunoassay for the assessment of hepatitis B virus core-related antigen following liver transplantation.2009
Author(s)
Fujimoto M, Ichikawa T, Nakao K, Miyaaki H, Shibata H, Eguchi S, Takatsuki M, Nagaoka S, Yatsuhashi H, Kanematsu T, Fguchi K
-
Journal Title
Internal medicine journal 48
Pages: 1577-1583
NAID
Related Report
Peer Reviewed
-
[Journal Article] Long-term increase in liver volume after Denver peritoneovenous shout : report of two cases.2009
Author(s)
Miyaaki H, Murakami E, Ichikawa T, Nakao K, Takeshita S, Shibata H, Ozawa E, Akiyama M, Miuma S, Fujimoto M, Kuroki T, Tajima Y, Kanematsu T, Eguchi K
-
Journal Title
Liver Internationa 29
Pages: 774-775
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
[Presentation] Induction of hepatic progenitor cells by transplanted rat dermal fibroblast allograft sheet on an incised rat liver2011
Author(s)
Muraoka I, Soyama A, Takatsuki M, Tomonaga T, Hidaka M, Kanematsu T, Hishikawa Y, Koji T, Utoh R, Ohashi K, Eguchi S
Organizer
62^<nd> AASLD
Place of Presentation
SF, USA
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-