Development of boronated peptide using anti-oxidative effect for neutron capture therapy

• Project/Area Number: 21659337
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: University of Tsukuba
• Principal Investigator: MATSUMURA Akira 筑波大学, 医学医療系, 教授 (90241819)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Tetsuya 筑波大学, 医学医療系, 講師 (30375505) ; NAKAI Kei 筑波大学, 医学医療系, 講師 (50436284) ; YOSHIDA Fumiyo 筑波大学, 医学医療系, 助教 (30261811)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,430,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 2009: ¥800,000 (Direct Cost: ¥800,000)
• Keywords: 中性子捕捉療法 / ホウ素中性子捕捉療法 / 抗酸化作用 / ホウ素化合物 / グルタチオン

Research Abstract

We synthesize novel peptide which consist of boronated amino-acid. We succeed the synthesis pentamer and decamer of bolonophenylalanine by fluorenylmethoxycarbonyl (Fmoc)-based solid-phase peptide synthesis. And we developed novel lipopeptide which containing boron, and liposome with it. We found the pre-treatment of glutathione modified the in vivo distribution of boron compounds.

Research Products

• [Journal Article] Matsumura A A clinicaltrial protocol for second linetreatment of malignant brain tumorswith BNCT at University of Tsukuba (2011)
  • Author(s): Aiyama H, Nakai K, Yamamoto T, Nariai T, Kumada H, Ishikawa E, Isobe T, Endo K, Takada T, YoshidaF, Shibata Y
  • Journal Title: Appl Radiat Isot Volume: 69 Pages: 1819-1822
  • Peer Reviewed
• [Journal Article] Matsumura A Boron neutron capture therapy combined with fractionated photon irradiation for glioblastoma (2011)
  • Author(s): Nakai K, Yamamoto T, Aiyama H, Takada T, Yoshida F, Kageji T, Kumada H, Isobe T, Endo K, MatsudaM, Tsurubuchi T, Shibata Y, TakanoS, Mizumoto M, Tsuboi K
  • Journal Title: arecursive partitioning analysis of BNCT patients Appl Radiat Isot Volume: 69 Pages: 1790-1792
  • Peer Reviewed
• [Journal Article] Boron neutron capture therapy combined with fractionated photon irradiation for glioblastoma : a recursive partitioning analysis of BNCT patients (2011)
  • Author(s): Nakai K, Yamamoto T, Aiyama H, Takada T, Yoshida F, Kageji T, Kumada H, Isobe T, Endo K, Matsuda M, Tsurubuchi T, Shibata Y, Takano S, Mizumoto M, Tsuboi K, Matsumura A
  • Journal Title: Appl Radiat Isot Volume: 69 Issue: 12 Pages: 1790-1792
  • DOI: 10.1016/j.apradiso.2011.03.049
  • Peer Reviewed
• [Journal Article] A clinical trial protocol for second line treatment of malignant brain tumors with BNCT at University of Tsukuba (2011)
  • Author(s): Aiyama H, Nakai K, Yamamoto T, Nariai T, Kumada H, Ishikawa E, Isobe T, Endo K, Takada T, Yoshida F, Shibata Y, Matsumura A
  • Journal Title: Appl Radiat Isot Volume: 69 Issue: 12 Pages: 1819-1822
  • DOI: 10.1016/j.apradiso.2011.04.031
  • Peer Reviewed
• [Journal Article] Intracellular uptakeof a new boronated porphyrin EC032 (2009)
  • Author(s): Tsurubuchi T, Yamamoto T, Nakai K, Zaboronok A, Yoshida F, Miyakawa M, Shirakawa M, Yamamoto Y, Matsuda M, Matsumura A
  • Journal Title: Appl Radiat Isot Volume: 67 Pages: 94-6
  • Peer Reviewed
• [Journal Article] Synthesis and evaluation of a novel liposome containing BPA-peptide conjugate for BNCT. (2009)
  • Author(s): Shirakawa M
  • Journal Title: Appl Radiat Isot 67(7-8 suppl) Pages: 84-7
  • Peer Reviewed
• [Presentation] MatsumuraA Evaluation of liposome modified to Boron-lipopeptide for BNCT (2012)
  • Author(s): Shirakawa M, Nakai K
  • Organizer: Workshop BNCT DAAD projectresearch and development in BNCT and charged particle therapy
  • Place of Presentation: Mainz, Germany
  • Year and Date: 2012-03-02
• [Presentation] Clinical Trial of BNCT in University Tsukuba (2012)
  • Author(s): Nakai K, Shirakawa M, Matsumura A
  • Organizer: Workshop "BNCT" DAAD project "research and development in BNCT and charged particle therapy"
  • Place of Presentation: Mainz (Germany)
  • Year and Date: 2012-03-02
• [Presentation] Evaluation of liposome modified to Boron-lipopeptide for BNCT (2012)
  • Author(s): Shirakawa M, Nakai K, Matsumura A
  • Organizer: Workshop "BNCT" DAAD project "research and development in BNCT and charged particle therapy"
  • Place of Presentation: Mainz (Germany)
  • Year and Date: 2012-03-02
• [Presentation] Clinical Trial of BNCT in University Tsukuba (2012)
  • Author(s): Shirakawa M, Nakai K, Matsumura A
  • Organizer: Workshop High LET particle therapyDAAD project research and development in BNCT and charged particle therapy
  • Place of Presentation: Marburg Germany
  • Year and Date: 2012-03-01
• [Presentation] Clinical Trial of BNCT in University Tsukuba (2012)
  • Author(s): Nakai K, Shirakawa M, Matsumura A
  • Organizer: Workshop "High LET particle therapy" DAAD project "research and development in BNCT and charged particle therapy"
  • Place of Presentation: Marburg (Germany)
  • Year and Date: 2012-03-01
• [Presentation] Evaluation of liposome modifiedto Boron-lipopeptide for BNCT (2012)
  • Author(s): Shirakawa M, Nakai K, Matsumura A
  • Organizer: Clinical Trial of BNCT in University Tsukuba Workshop Modern particle Therapy DAAD project research and development in BNCT and charged particle therapy
  • Place of Presentation: Essen Germany
  • Year and Date: 2012-02-28
• [Presentation] Clinical Trial of BNCT in University (2012)
  • Author(s): Nakai K, Shirakawa M, Matsumura A
  • Organizer: Tsukuba Workshop Modern particleTherapy DAAD project researchand development in BNCT and chargedparticle therapy
  • Place of Presentation: EssenGermany
  • Year and Date: 2012-02-28
• [Presentation] Clinical Trial of BNCT in University Tsukuba (2012)
  • Author(s): Nakai K, Shirakawa M, Matsumura A
  • Organizer: Workshop "Modern particle Therapy" DAAD project "research and development in BNCT and charged particle therapy"
  • Place of Presentation: Essen (Germany)
  • Year and Date: 2012-02-28
• [Presentation] Evaluation of liposome modified to Boron-lipopeptide for BNCT (2012)
  • Author(s): Shirakawa M, Nakai K, Matsumura A
  • Organizer: Workshop "Modern particle Therapy" DAAD project "research and development in BNCT and charged particle therapy"
  • Place of Presentation: Essen (Germany)
  • Year and Date: 2012-02-28
• [Presentation] Yoshida F (2011)
  • Author(s): Shirakawa M, Nakai K, Yamamoto T
  • Organizer: Matsumura A evaluation of liosome modified to Boron-lipopeptide for BNCT 6 thyoung researchers BNCT meeting
  • Place of Presentation: Taiwan
  • Year and Date: 2011-12-06
• [Presentation] evaluation of liosome modified to Boron-lipopeptide for BNCT (2011)
  • Author(s): Shirakawa M, Nakai K, Yamamoto T, Yoshida F, Matsumura A
  • Organizer: 6th young researchers BNCT meeting
  • Place of Presentation: Taiwan Hsinchu
  • Year and Date: 2011-12-06
• [Presentation] Matsumura A et al (2010)
  • Author(s): Shirakawa M, Yamamoto T, Nakai K, Yoshida F
  • Organizer: Development of a fuctional liposome modified a novel lipid analog for BNCT. 14th International congresson neutron capture therapy
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2010-10-25
• [Presentation] et al Biodistributionof BSH-encapsulated boron liposomein mouse glioma (2010)
  • Author(s): Yoshida F, Nakai K, Yamamoto T, Matsumura A
  • Organizer: 14th International congress on neutron capture therapy
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2010-10-25
• [Presentation] Biodistribution of BSH-encapsulated boron liposome in mouse glioma. (2010)
  • Author(s): Yoshida F, Nakai K, Yamamoto T, Matsumura A, et. al.
  • Organizer: 14th International congress on neutron capture therapy
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2010-10-25
• [Presentation] Development of a fuctional liposome modified a novel lipid analog for BNCT. (2010)
  • Author(s): Shirakawa M, Yamamoto T, Nakai K, Yoshida F, Matsumura A, et. al.
  • Organizer: 14th International congress on neutron capture therapy
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2010-10-25
• [Presentation] ほか、脳腫瘍に対するBNCT:筑波大学臨床研究の現況 (2010)
  • Author(s): 中井啓, 山本哲哉, 松村明
  • Organizer: 第7回日本中性子捕捉療法学会学術集会
  • Year and Date: 2010-08-05
• [Presentation] 脳腫瘍に対するBNCT:筑波大学臨床研究の現況 (2010)
  • Author(s): 中井啓, 山本哲哉, 松村明, ほか
  • Organizer: 第7回日本中性子捕捉療法学会学術集会
  • Place of Presentation: 東京
• [Presentation] et al clinicalresult and adjuvant therapy for glioblastoma with BNCT treatedpatients (2009)
  • Author(s): Nakai K, Matsumura A
  • Organizer: Young Researchers BNCTmeeting
  • Place of Presentation: Mainz, Germany
  • Year and Date: 2009-09-29
• [Presentation] et al Evaluation of a novel liposome modified membrane permeability peptide for BNCT (2009)
  • Author(s): Shirakawa M, Matsumura A
  • Organizer: Young Researchers BNCT meeting
  • Place of Presentation: Mainz, Germany
  • Year and Date: 2009-09-29
• [Presentation] clinical result and adjuvant therapy for glioblastoma with BNCT treated patients. (2009)
  • Author(s): Nakai K, Matsumura A, et al
  • Organizer: Young Researchers BNCT meeting
  • Place of Presentation: Mainz, Germany
  • Year and Date: 2009-09-29
• [Presentation] Evaluation of a novel liposome modified membrane permeabiltiy peptide for BNCT. (2009)
  • Author(s): Shirakawa M, Matsumura A, et al
  • Organizer: Young Researchers BNCT meeting
  • Place of Presentation: Mainz, Germany
  • Year and Date: 2009-09-29