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運動器再生医療を目指した多能性幹細胞から間葉系細胞への分化誘導シグナルの探索

Research Project

Project/Area Number 21659347
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionThe University of Tokyo

Principal Investigator

中村 耕三  東京大学, 医学部附属病院, 教授 (60126133)

Co-Investigator(Kenkyū-buntansha) 松原 全宏  東京大学, 医学部附属病院, 助教 (40361498)
齋藤 琢  東京大学, 医学部附属病院, 特任准教授 (30456107)
Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2009: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywords四肢機能再建学 / 幹細胞
Research Abstract

間葉系マーカーであるPRX1、PRX2の種間で高度に保存されている近位3kbの配列を用いたルシフェラーゼレポーターアッセイにて、NFkBシグナルの主な転写因子であるRELAがPRX1、PRX2をともに強力に活性化し、またCREB/ATFファミリー分子がPRX1プロモーターを、KLFファミリー分子がPRX2プロモーターを、それぞれ選択的に強力に活性化することが判明した。プロモーターのデリーションアッセイにてそれぞれの応答部位を同定した。Prx1については、さらにその応答部位を中心に40bpほどの配列をタンデムに重ねてベーサルプロモーターに繋げ、蛍光蛋白EGFPを接合させることで、強力な蛍光レポーターカセットを作成した。これらをレンチウイルスベースに組み替え、iPS細胞に安定導入することを試みたが成功に至らなかったため、iPS樹立前の体細胞に一旦安定導入してからiPS細胞を樹立した。このiPS細胞はレチノイン酸をベースにした間葉系分化誘導法によって良好な発光を呈したため、この蛍光モニタリングシステムを用いて発現クローニングを行い、複数の間葉系誘導能を持つと予想される候補分子を得た。このうち転写因子p53のファミリー分子については、未分化間葉系細胞の凝集段階において強く発現することが確かめられた。しかしながらこの分子については発現ベクターを作成してウイルスベースで導入してもマウスES細胞やiPS細胞を間葉系に誘導する有効な効果は認められなかった。現在は他の候補分子について発現と機能の両面から解析を行っている。

Report

(2 results)
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (28 results)

All 2011 2010 2009 Other

All Journal Article (23 results) (of which Peer Reviewed: 23 results) Presentation (2 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Association of Knee Osteoarthritis with the Accumulation of Metabolic Risk Factors Such as Overweight, Hypertension, Dyslipidemia, and Impaired Glucose Tolerance in Japanese Men and Women : The ROAD Study.2011

    • Author(s)
      Yoshimura N, Nakamura K, et al.
    • Journal Title

      J Rheumatol

      Volume: Epub

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduction of Peritendinous adhesions by hydrogel containing biocompatible phospholipid polymer MPC for tendon repair.2011

    • Author(s)
      Ishiyama N, Nakamura K, et al.
    • Journal Title

      J Bone Joint Surg Am

      Volume: 93 Pages: 142-9

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acute Cervical Spinal Cord Injury Complicated by Pre-existing Ossification of the Posterior Longitudinal Ligament : A Multi-center Study.2011

    • Author(s)
      Chikuda H, Nakamura K, et al.
    • Journal Title

      Spine

      Volume: Epub

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Capacity of endogenous sex steroids to predict bone loss in Japanese men : 10-year follow-up of the Taiji Cohort Study.2011

    • Author(s)
      Yoshimura N, Nakamura K, et al.
    • Journal Title

      J Bone Miner Metab

      Volume: 29 Pages: 96-102

    • NAID

      10029023846

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A local application of recombinant human fibroblast growth factor 2 for tibial shaft_fractures : A randomized, placebo-controlled trial.2010

    • Author(s)
      Kawaguchi H, Nakamura K, et al.
    • Journal Title

      J Bone Miner Res

      Volume: 25 Pages: 2459-67

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional regulation of endochondral ossification by HIF-2alpha during skeletal growth and osteoarthritis development.2010

    • Author(s)
      Saito T, Nakamura K, et al.
    • Journal Title

      Nat Med

      Volume: 16 Pages: 678-86

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of knee and low back pain on health-related quality of life in Japanese women : the Research on Osteoarthritis Against Disability (ROAD).2010

    • Author(s)
      Muraki S, Nakamura K, et al.
    • Journal Title

      Mod Rheumatol

      Volume: 20 Pages: 444-51

    • NAID

      10027646934

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of oxytetracycline as a chondrogenic compound using a cell-based screening system.2010

    • Author(s)
      Hojo H, Nakamura K, et al.
    • Journal Title

      J Bone Miner Metab

      Volume: 28 Pages: 627-33

    • NAID

      10027845798

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Predictors of residual symptoms in lower extremities after decompression surgery on lumbar spinal stenosis.2010

    • Author(s)
      Hara N, Nakamura K, et al.
    • Journal Title

      Eur Spine J

      Volume: 19 Pages: 1849-54

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correlation between pyramidal signs and the severity of cervical myelopathy.2010

    • Author(s)
      Chikuda H, Nakamura K, et al.
    • Journal Title

      Eur Spine J

      Volume: 19 Pages: 1684-9

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The prevention of peritendinous adhesions by a phospholipid polymer hydrogel formed in situ by spontaneous intermolecular interactions.2010

    • Author(s)
      Ishiyama N, Nakamura K, et al.
    • Journal Title

      Biomaterials

      Volume: 31 Pages: 4009-16

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aktl in murine chondrocytes controls cartilage calcification during endochondral ossification under physiologic and pathologic conditions2010

    • Author(s)
      Fukai A, Nakamura K, et al.
    • Journal Title

      Arthritis Rheum 62

      Pages: 826-36

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The prevention of peritendinous adhesions by a phospholipid polymer hydrogel formed in situ by spontaneous intermolecular interactions.2010

    • Author(s)
      Ishiyama N, Nakamura K, et al.
    • Journal Title

      Biomaterials 31

      Pages: 4009-16

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms underlying catabolic and anabolic functions of parathyroid hormone on bone by combination of culture systems of mouse cells.2010

    • Author(s)
      Shinoda Y, Nakamura K, et al.
    • Journal Title

      J Cell Biochem 109

      Pages: 755-63

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Predictors of residual symptoms in lower extremities after decompression surgery on lumbar spinal stenosis.2010

    • Author(s)
      Hara N, Nakamura K, et al.
    • Journal Title

      Eur Spine J (E pub)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correlation between pyramidal signs and the severity of cervical myelopathy.2010

    • Author(s)
      Chikuda H, Nakamura K, et al.
    • Journal Title

      Eur Spine J (E pub)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Screening of chondrogenic factors with a real-time fluorescence-monitoring cell line ATDC5-C2ER : identification of sorting nexin 19 as a novel factor.2009

    • Author(s)
      Kan A, Nakamura K, et al.
    • Journal Title

      Arthritis Rheum 60

      Pages: 3314-23

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-apoptotic molecule Bcl-2 regulates the differentiation, activation, and survival of both osteoblasts and osteoclasts2009

    • Author(s)
      Nagase Y, Nakamura K, et al.
    • Journal Title

      J Biol Chem 284

      Pages: 36659-69

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The antiapoptotic protein Bcl-xL negatively regulates the bone-resorbing activity of osteoclasts in mice2009

    • Author(s)
      Iwasawa M, Nakamura K, et al.
    • Journal Title

      J Clin Invest 119

      Pages: 3149-59

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Orphan receptor tyrosine kinase ROR2 as a potential therapeutic target for osteosarcoma.2009

    • Author(s)
      Morioka K, Nakamura K, et al.
    • Journal Title

      Cancer Sci 100

      Pages: 1227-33

    • NAID

      10027191977

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sleep apnea in rheumatoid arthritis patients with occipitocervical lesions : the prevalence and associated radiographic features.2009

    • Author(s)
      Shoda N, Nakamura K, et al.
    • Journal Title

      Eur Spine J 18

      Pages: 905-10

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diameter, length, and direction of pedicle screws for scolic tic spine : analysis by multiplanar reconstruction of computed tomography2009

    • Author(s)
      Takeshita K, Nakamura K, et al.
    • Journal Title

      Spine 34

      Pages: 798-803

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wear resistance of artificial hip joints with poly(2-methacryloyloxyethyl phosphorylcholine)grafted polyethylene : comparisons with the effect of polyethylene cross-linking and ceramic femoral heads.2009

    • Author(s)
      Moro T, Nakamura K, et al.
    • Journal Title

      Biomaterials 30

      Pages: 2995-3001

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] A transcription factor p63 controls extensive steps of endochondral ossification through distinct functions of the isoforms.2010

    • Author(s)
      Taniguchi Y, Saito T, Nakamura K, et al.
    • Organizer
      32th annual meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2010-10-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Comprehensive control of endochondral ossification by HIF-2α during skeleta growth and osteoarthritis progression.2009

    • Author(s)
      Saito T, Nakamura K, et al.
    • Organizer
      31th annual meeting of the American Society for Bone and Mineral Research(ASBMR).
    • Place of Presentation
      Denver, CO, USA
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Book] 新国民病ロコモティブシンドローム長寿社会は警告する2010

    • Author(s)
      中村耕三
    • Total Pages
      193
    • Publisher
      NHK出版生活人新書315
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.h.u-tokyo.ac.jp/ortho/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.h.u-tokyo.ac.jp/ortho/

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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