| Project/Area Number |
21659417
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Emergency medicine
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,210,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2009: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | 急性中毒学 / 酸化ストレスマーカー / アセトアミノフェン中毒 / 8-OHdG / MDA / 酸化ストレスーカー / 8-hydroxy deoxyguanosine / N-アセチルシステイン / グルタチオン / マロンジアルデヒド |
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| Research Abstract |
Acetaminophen(APAP) is rapidly absorbed from gastrointestinal tract, and approximately 4% of any ingested amount is metabolized by hepatic cytochrome P450 mixed function oxidase system to an active intermediate metabolite that is thought to produce hepatotoxicity. This metabolite, N-acetyl p-benzoquinone mine(NAPQI), is normally detoxified by conjugation with glutathione and excreted by the urine. After an overdose, when glutathione is rapidly used and the store drop to less than 30% of normal, the unconjugated toxic metabolite may bind covalently to various hepatocellular macromolecules producing necrosis.
To propose the novel biomarker for efficacy of antidote in acetaminophen poisoning, we have evaluated some markers such as total antioxidant power(TAP), 8-hydroxy deoxyguanosine(8-OH-dG) and GSH in RBC and also developed a sensitive HPLC method for measuring malonedialdehyde(MDA) in urine and serum.
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