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THE DEVELOPMENT OF NEWLY JAPAN-ORIGINAL GENE THERAPY FOR DEGENERATIVE ARTHROSIS USING DECOY GENE.

Research Project

Project/Area Number 21659433
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionShimane University

Principal Investigator

ISHIBASHI Hiroaki  島根大学, 医学部, 准教授 (90254630)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,270,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2009: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords免疫 / 感染 / 炎症 / 細胞外基質 / 顎関節 / 遺伝子治療 / 顎関節症 / 基質破壊酵素 / 遣伝子治療
Research Abstract

Examination for the expression of uPA and MMPs stimulated by several cytokines using cultured cells. The expression of uPA and MMPs was increased by the stimulation with TNFαusing cultured cells derived from cartilaginous tissue of TMJ. Examination of transcriptional factor related to cytokines stimulation. Transcriptional factors, NF-kB and Sp1, were activated by TNFαstimulation at the cultured cells derived from cartilaginous tissue. Establishment of the condition for the gene transfer We established the condition for the Decoy oligodeoxynucletides targeted to NF-kB and Sp1 using HVJ-liposome method, and decoy ODNs were routinely transferred to 100% of the cells. Inhibition of the expression of uPA and MMPs by Decoy ODNs transfection The expression of the uPA and MMPs were inhibited by the transfection of Decoy ODNs to the cultured cells derived from cartilaginous tissue.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (10 results)

All 2012 2011 2010 2009 Other

All Journal Article (7 results) (of which Peer Reviewed: 3 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] Oral symptoms including dental erosion in gastroesophageal reflux disease are associated with decreased salivary flow volume and swallowing function2012

    • Author(s)
      Yoshikawa H, et al.
    • Journal Title

      Journal of Gastroenterology

      Volume: 27 Pages: 124-128

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A pediatric case of unicystic ameloblastoma treated with conservative suegery leading to eruption of the impacted permanent teeth2011

    • Author(s)
      Kanno T, Ishibashi H, Sukegawa S, Tsunematsu K, Nakamura S, Mano S, Sekine J, Furuki Y
    • Journal Title

      Hospital Dentistry and Oral-Maxillofacial Surgery

      Volume: 23(2) Pages: 129-132

    • Related Report
      2011 Final Research Report
  • [Journal Article] Our approach for early detection of oral cancer using smear cytology2010

    • Author(s)
      Kikuchi M, Ishibashi H, et al.
    • Journal Title

      Korean Association of Oral and Maxillofacial Surgeons

      Volume: 36 Pages: 315-316

    • Related Report
      2010 Annual Research Report
  • [Journal Article] A fish bone in the tongue2010

    • Author(s)
      Nariai Y, Yanai C, Kondo S, Ishibashi H, et al.
    • Journal Title

      Asian J Oral Maxillofac Surg

      Volume: 22 Pages: 30-32

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Management of tongue squamous cell carcinoma in pregnant women : Report of two Patients2009

    • Author(s)
      Kondo S, Ishibashi H, Nariai Y, Kato T, Enomoto Y, Karino M, Sekine J
    • Journal Title

      Asian J Oral Maxillofac Surg

      Volume: 21 Pages: 129-132

    • Related Report
      2011 Final Research Report
  • [Journal Article] Autocrine loop between vascular endothelial growth factor(VEGF)-C and VEGF receptor-3 positively regulates tumor-associated lymphangio-genesis in oral squamoid cancer cells2009

    • Author(s)
      Matsuura M, Onimaru M, Yonemitsu Y, Suzuki H, Nakano T, Ishibashi H, Shirasuna K, Sueishi K
    • Journal Title

      Am J Pathology

      Volume: 174 Pages: 361-368

    • Related Report
      2011 Final Research Report
  • [Journal Article] Autocrine loop between vascular endothelial growth factor (VEGF)-C and VEGF Receptor-3 positively regulates tumor-associated lymphangiogenesis in oral squamoid cancer cells.2009

    • Author(s)
      Matsuura M, Onimaru M, Yonemitsu Y, Suzuki H, Nakano T, Ishibashi H, et al.
    • Journal Title

      American Journal of Pathology 175(4)

      Pages: 1709-1721

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 「Novel gene therapy」Newly regulation strategy against tumor angiogenesis and chemosensitivity using decoy system2011

    • Author(s)
      Ishibashi, H. Koike, H. Hideshima, K. Karino, M. Tunematsu, K, Sekine J.
    • Organizer
      4^<th> Internatinal Conference of Advanced Digital Technology in Head and Neck reconstruction
    • Place of Presentation
      Freiburg
    • Year and Date
      2011-05-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Newly gene therapy for temporomandibular joint arthritis by in vitro cis-transelement decoy against TNFα-induced NF-kB activation2011

    • Author(s)
      Ishibashi H, et al.
    • Organizer
      4^<th> Advanced Digital Conference
    • Place of Presentation
      Freiburg, Germany(招待講演)
    • Year and Date
      2011-05-05
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.shimane-u.ac.jp/oral/

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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