Analysis of microRNAs and their target genes associated with phenotypes of oral squamous cell carcinomas.

• Project/Area Number: 21659469
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Kochi University
• Principal Investigator: YAMAMOTO Tetsuya 高知大学, 教育研究部・医療学系, 教授 (00200824)
• Co-Investigator(Kenkyū-buntansha): SASABE Eri 高知大学, 教育研究部・医療学系, 助教 (40363288) ; MORISHITA Keiko 高知大学, 教育研究部・医療学系, 助教 (10527584)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2009: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: 口腔扁平上皮癌 / マイクロRNA / 悪性形質 / エクソソーム

Research Abstract

We investigated the expression of microRNAs in oral squamous cell carcinoma(OSCC) cell lines and normal oral keratinocytes by using microarray analysis and real time-PCR. It was demonstrated that the expression of 9 microRNAs was up-regulated and that of 13 microRNAs was down-regulated in OSCC cells compared with normal cells. We also revealed that miR-34a, one of down-regulated genes, acts as a tumor suppressor gene in OSCC cells and that the expression of the microRNA is down-regulated in OSCC tissues.

Research Products

• [Journal Article] The involvement of hypoxia-inducible factor-1α in the susceptibility to γ-rays and chemotherapeutic drugs of oral squamous cell carcinoma cells (2010)
  • Author(s): Eri sasabe, Xuan Zhou, Dechao Li, Naohisa Oku, Tetsuya yamamoto, Tokio osaki
  • Journal Title: Recent Advances and Research Updates 11 Pages: 121-136
• [Journal Article] ビスフォスフォネート投与と関連性があると考えられた顎骨骨髄炎ならびに顎骨壊死30症例に関する追跡調査～2年後の現状について～ (2009)
  • Author(s): 浦出雅裕, 田中徳昭, 嶋田淳, 柴田孝典, 古澤清文, 桐田忠昭, 山本哲也, 渡辺哲郎, 北川善政, 倉科憲治, 瀬戸宗一, 福田仁一
  • Journal Title: 日本口腔外科学会雑誌 55巻 Pages: 553-561
  • NAID: 10027091304
  • Peer Reviewed
• [Presentation] The down-regulated micro RNA in oral squamous cell carcinoma acts as a tumor suppressor (2011)
  • Author(s): Tomohide Yoshimura, Ge Huabing, Wang Jianguo, Eri Sasabe, Tomohiro Yamada, Tetsuya Yamamoto
  • Organizer: American Association of Oral and Maxillofacial Surgeons. 2011 Annual Meeting
  • Place of Presentation: Philadelphia Convention Center, Philadelphia, USA
  • Year and Date: 2011-09-18
• [Presentation] The down-regulated micro RNA in oral squamous cell carcinoma acts as a tumor suppressor (2011)
  • Author(s): Tomohide Yoshimura, Ge Huabing, Wang Jianguo, Eri Sasabe, Tomohiro Yamada, Tetsuya Yamamoto
  • Organizer: American Association of Oral and Maxillofacial Surgeons 2011 Annual Meeting
  • Place of Presentation: Philadelphia Convention Center, Philadelphia, USA
  • Year and Date: 2011-09-18
• [Presentation] 口腔扁平上皮癌細胞の放射線感受性に及ぼすマイクロRNAの影響に関する検討 (2011)
  • Author(s): 吉村友秀、笹部衣里、葛化冰、北村直也、山本哲也
  • Organizer: 第65回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-04-21
• [Presentation] 口腔扁平上皮癌細胞の放射線感受性に及ぼすマイクロRNAの影響に関する検討 (2011)
  • Author(s): 吉村友秀、笹部衣里、葛化冰、北村直也、山本哲也
  • Organizer: 第65回NPO法人日本口腔科学会学術集会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2011-04-21
• [Presentation] 口腔扁平上皮癌におけるマイクロRNAの発現と増殖・浸潤における役割についての検討 (2010)
  • Author(s): 吉村友秀、笹部衣里、北村直也、山本哲也
  • Organizer: 第47回日本口腔組織培養学会学術大会
  • Place of Presentation: 高知
  • Year and Date: 2010-11-13
• [Presentation] 口腔扁平上皮癌におけるマイクロRNAの発現と増殖・浸潤における役割についての検討 (2010)
  • Author(s): 吉村友秀、笹部衣里、北村直也、山本哲也
  • Organizer: 第47回日本口腔組織培養学会学術大会
  • Place of Presentation: 高知城ホール(高知)
  • Year and Date: 2010-11-13
• [Presentation] 口腔扁平上皮癌細胞におけるマイクロRNAの発現変化ならびにその機能 (2010)
  • Author(s): 吉村友秀、笹部衣里、森下慶子、山田朋弘、山本哲也
  • Organizer: 第64回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-06-25
• [Presentation] 口腔扁平上皮癌におけるマイクロRNAの発現と増殖・浸潤における役割についての検討 (2010)
  • Author(s): 吉村友秀、笹部衣里、森下慶子、山田朋弘、山本哲也
  • Organizer: 第64回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 札幌プリンスホテル国際館パミール(札幌)
• [Book] 口腔外科学(第3版) (2010)
  • Author(s): 山本哲也
  • Total Pages: 21
  • Publisher: 医歯薬出版株式会社
• [Remarks]
  • URL: http://www.kochi-ms.ac.jp/~fm_dntst/index.htm
• [Remarks]
  • URL: http://www.kochi-ms.ac.jp/~fm_dntst/index.htm