| Project/Area Number |
21659477
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Porphyromonas gingivalis / Streptococcus mutans / 分子標的治療 / PNA / ペプチド核酸(PNA) / アンチセンス法 / 膜透過型ペプチド / 歯周病 / 齲蝕 / Aggregatibacter actinomycetemcomitans |
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| Research Abstract |
There has been no report that antisense method is applied to causative bacteria of the periodontal disease. In this study, we investigated whether antisense method was available for those causative bacteria by using PNA that was the artificial nucleic acid and was not affected by RNase. Antisense PNA was reacted with Porphyromonas gingivalis that was causative organism of the periodontitis that was one of the periodontal diseases. As a result, we did not obtain the expected antisense effect because the strong peptidase activity of P. gingivalis hydrolyzed the membrane transport guidance protein or the main body of PNA constructed of peptide bonds. On the other hand, antisense PNA against Streptococcus mutans that was carious causative bacteria tended to suppress the targeted gtfB gene expression although the significant difference was not shown. In our future study, it is necessary to investigate the peptide sequence that facilitate for PNA to penetrate bacterial membrane and the antisense PNA sequence that inhibit the targeted gene expression efficiently.
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