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Pursuit of multiplication control of the periodontosis bacillus by a new molecule target cure, and the possibility as the anti-periodontosis medicine

Research Project

Project/Area Number 21659477
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Orthodontic/Pediatric dentistry
Research InstitutionNagasaki University

Principal Investigator

HOSHINO Tomonori  長崎大学, 病院, 講師 (00359960)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsPorphyromonas gingivalis / Streptococcus mutans / 分子標的治療 / PNA / ペプチド核酸(PNA) / アンチセンス法 / 膜透過型ペプチド / 歯周病 / 齲蝕 / Aggregatibacter actinomycetemcomitans
Research Abstract

There has been no report that antisense method is applied to causative bacteria of the periodontal disease. In this study, we investigated whether antisense method was available for those causative bacteria by using PNA that was the artificial nucleic acid and was not affected by RNase. Antisense PNA was reacted with Porphyromonas gingivalis that was causative organism of the periodontitis that was one of the periodontal diseases. As a result, we did not obtain the expected antisense effect because the strong peptidase activity of P. gingivalis hydrolyzed the membrane transport guidance protein or the main body of PNA constructed of peptide bonds. On the other hand, antisense PNA against Streptococcus mutans that was carious causative bacteria tended to suppress the targeted gtfB gene expression although the significant difference was not shown. In our future study, it is necessary to investigate the peptide sequence that facilitate for PNA to penetrate bacterial membrane and the antisense PNA sequence that inhibit the targeted gene expression efficiently.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (8 results)

All 2012 2010 2009

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] Novel Epitopic Region of Glucosyltransferase B from Streptococcus mutans2012

    • Author(s)
      Tomonori Hoshino, Yoshio Kondo, Kan Saito, Yutaka Terao, Nobuo Okahashi, Shigetada Kawabata, and Taku Fujiwara, Shigetada Kawabata, and Taku Fujiwara
    • Journal Title

      Clinical and Vaccine Immunology

      Volume: 18(9) Issue: 9 Pages: 1552-1561

    • DOI

      10.1128/cvi.05041-11

    • NAID

      120006985025

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of Streptococcus mutans in infants induces decrease in the proportion of salivaryα-haemolytic bacteria2012

    • Author(s)
      Mamoru Kawaguchi, * Tomonori Hosohino, Takashi Ooshima, Taku Fujiwara
    • Journal Title

      International Journal of Paediatric Dentistry

      Volume: 22(2) Issue: 2 Pages: 139-145

    • DOI

      10.1111/j.1365-263x.2011.01183.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Presentation] Horizontal Transmission of Glucosyltransferase Genes in Streptococcus mutans2012

    • Author(s)
      Hoshino, T., Kawabata, S., Fujiwara, T.
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Novel Epitopic region of Glucosyltransferase-B from Streptococcus mutans MT81482010

    • Author(s)
      Tomonori Hoshino, Kan Saito, Taku Fujiwara
    • Organizer
      the 88th IADR General Session & Exhibition
    • Place of Presentation
      Barcelona(Spain)
    • Related Report
      2011 Final Research Report
  • [Presentation] Horizontal Transmission of Glucosyltransferase Genes in Streptococcus mutans2010

    • Author(s)
      Tomonori Hoshino, Shigetada Kawabata, Taku Fujiwara
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Related Report
      2011 Final Research Report
  • [Presentation] Novel Epitopic region of Glucosyltransferase-B from Streptococcusmutans MT81482010

    • Author(s)
      Tomonori Hoshino, Kan Saito, Take Fujiwara
    • Organizer
      the 88th IADR General Session & Exhibition
    • Place of Presentation
      バルセロナ(スペイン)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Streptococcus mutansグルコシルトランスフェラーゼの新規エピトープとこれにより誘導された抗血清による活性抑制効果について2009

    • Author(s)
      星野倫範, 齋藤幹, 藤原卓
    • Organizer
      第47回日本小児歯科学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2009-05-14
    • Related Report
      2011 Final Research Report
  • [Presentation] Streptococcus mutansグルコシルトランスフェラーゼの新規エピトープとこれにより誘導された抗血清による活性抑制効果について2009

    • Author(s)
      夏野倫範, 齋藤幹, 藤源卓
    • Organizer
      第47回日本小児歯科学会大会
    • Place of Presentation
      大阪大学コンベンションセンター(大阪)
    • Year and Date
      2009-05-14
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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