| Budget Amount *help |
¥69,550,000 (Direct Cost: ¥53,500,000、Indirect Cost: ¥16,050,000)
Fiscal Year 2012: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2011: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2010: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2009: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
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| Research Abstract |
Germline development and meiosis are pivotal for all genetic events, and elucidation of the molecular mechanisms is an important subject for both basic and applied genetics. Here I used genetic resources of rice, a model crop, and analyzed the functions of two RNA-binding proteins, MEL1 and MEL2. MEL1 is known to play important roles in early meiotic processes. In this study, 21-and 24-nucleotides small RNAs that bind to MEL1 (mipsRNAs) were identified. Mapping onto the rice genome revealed that the mipsRNAs were processed from 700 species of long intergenic non-coding RNAs (lincRNAs). MEL2 functions to promote mitosis-meiosis transition in appropriate timing. This study revealed that RNA recognition motif (RRM) of MEL2 preferentially binded to the AU-rich RNA sequences (UUAGUU(U/A)(U/G)(A/U/G)U), and that 249 rice genes conserved the AU-rich consensus sequence. Identification of genes directly targeted by MEL1 and MEL2 will open the door to understanding transcriptional or post-transcriptional regulatory networks mediated by RNA-binding proteins in plant reproduction and meiosis.
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