Molecular mechanism of inhibition of insect odor-gated ion channels

• Project/Area Number: 21680035
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology and muscle physiology
• Research Institution: The University of Tokyo
• Principal Investigator: SATO Koji 東京大学, 生産技術研究所, 特任講師 (20444101)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥25,870,000 (Direct Cost: ¥19,900,000、Indirect Cost: ¥5,970,000); Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2009: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
• Keywords: 嗅覚 / 味覚 / イオンチャネル / シグナル伝達 / 受容体 / 感覚生理学

Research Abstract

Insect olfactory receptors compose heteromeric ion channels activated by odorants. Receptor screening experiments revealed the genes encoding ionotropic receptors. I expressed the receptors in heterologous cells, and analyzed their activity. I found some compounds competed with a ligand at their binding site on receptors. The receptor screening of silk moth genome showed a sugar-regulated ion channel, in which structurally related sugar inhibited its binding site. Finally, mutagenesis experiment of olfactory receptor complex identified the amino acid residue contributing the function of channel pore formation.

Research Products

• [Journal Article] 昆虫の化学感覚器に発現するイオノトロピック型化学感覚受容体 (2012)
  • Author(s): 佐藤幸治
  • Journal Title: 比較生理生化学 Volume: 29 Pages: 50-57
  • NAID: 10030727634
  • Peer Reviewed
• [Journal Article] Amino acid residues contributing to function of the heteromeric insect olfactory receptor complex (2012)
  • Author(s): Nakagawa T, Pellegrino M, Sato K, Vosshall LB, Touhara K
  • Journal Title: PLoS ONE Volume: 7 Issue: 3 Pages: e32372-e32372
  • DOI: 10.1371/journal.pone.0032372
  • Peer Reviewed
• [Journal Article] Sugar-regulated cation channel formed by an insect gustatory receptor (2011)
  • Author(s): Sato K, Tanaka K, Touhara K
  • Journal Title: Proceeding of the National Academy of Science of the United States of America Volume: 108 Issue: 28 Pages: 11680-11685
  • DOI: 10.1073/pnas.1019622108
  • Peer Reviewed
• [Journal Article] Adiponectin and AdipoR1 regulate PGC-1α and mitochondria by Ca2+ and AMPK/SIRT1 (2010)
  • Author(s): Iwabu, M., Yamauchi, T., Okada-Iwabu, M., Sato, K., Nakagawa, T., Funata, M., Yamaguchi, M., Namiki, S., Nakayama, R., Tabata, M., Ogata, H., Kubota, N.,Takamoto, I., Hayashi, YK., Yamauchi, N., Waki, H., Fukayama, M., Nishino, I., Tokuyama, K., Ueki, K., Oike, Y., Ishii, S., Hirose, K., Shimizu, T., Touhara, K., and Kadowaki, T
  • Journal Title: Nature Volume: 464 Issue: 7293 Pages: 1313-1319
  • DOI: 10.1038/nature08991
  • Peer Reviewed
• [Journal Article] 昆虫の7回膜貫通型嗅覚受容体複合体が構成する匂い活性型イオンチャネル (2009)
  • Author(s): 佐藤幸治
  • Journal Title: 日本薬理学雑誌 Volume: 134 Pages: 248-253
  • NAID: 10025741872
• [Journal Article] Two chemoreceptors mediate developmental effects of dauer pheromone in C. elegans (2009)
  • Author(s): Kim K, Sato K, Shibuya M, Zeiger DM, Butcher RA, Ragains JR, Clardy J, Touhara K, Sengupta P
  • Journal Title: Science Volume: 326 Issue: 5955 Pages: 994-998
  • DOI: 10.1126/science.1176331
  • Peer Reviewed
• [Journal Article] Two chemoreceptors mediate developmental effects of dauer pheromone in C.elegans. (2009)
  • Author(s): Kyuhyung Kim
  • Journal Title: Science Volume: 326 Pages: 994-998
  • Peer Reviewed
• [Presentation] Insect chemosensory receptor channels (2010)
  • Author(s): Sato K
  • Organizer: The 58th Annual Meeting of the Entomological Society of America
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-12-12
• [Presentation] Insect chemosensory receptor channels. (2010)
  • Author(s): 佐藤幸治
  • Organizer: The 58^<th> Annual Meeting of the Entomological Society of America
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-12-12
• [Presentation] 匂い物質によるイオンチャネル型昆虫嗅覚受容体の競合阻害 (2010)
  • Author(s): 佐藤幸治
  • Organizer: 日本農芸化学会2010年度東京大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-30
• [Presentation] 匂い物質によるイオンチャネル型昆虫嗅覚受容体の競合阻害 (2010)
  • Author(s): 佐藤幸治
  • Organizer: 日本農芸化学会2010年度東京大会
  • Place of Presentation: 東京大学駒場キャンパス、東京
  • Year and Date: 2010-03-30
• [Presentation] Insect chemosensation via ligand-gated cation channels (2009)
  • Author(s): Sato K
  • Organizer: The European Chemoreception Research Organisation XIX Congress
  • Place of Presentation: Villasimius, Italy
  • Year and Date: 2009-09-24
• [Presentation] Insect chemosensation via ligand-gated cation channels. (2009)
  • Author(s): 佐藤幸治
  • Organizer: The European Chemoreception Research Organisation XIX Congress
  • Place of Presentation: Italy, Villasimius
  • Year and Date: 2009-09-24
• [Presentation] Putative amino acids involved in ion channel activities of insect olfactory receptors (2009)
  • Author(s): Nakagawa T, Sato K, Touhara K
  • Organizer: 11th European Symposium for Insect taste and olfaction
  • Place of Presentation: Villasimius, Italy
  • Year and Date: 2009-09-19
• [Presentation] Putative amino acids involved in ion channel activities of insectolfactory receptors (2009)
  • Author(s): 中川龍朗
  • Organizer: 11^<th> European Symposium for Insect taste and olfaction
  • Place of Presentation: Italy, Villasimius
  • Year and Date: 2009-09-19
• [Book] 化学物質に対する昆虫の反応行動とその役割、化学受容の科学 (2012)
  • Author(s): 佐藤幸治
  • Publisher: 化学同人、京都
• [Book] 化学受容の科学-匂い・味・フェロモン分子から行動まで (2012)
  • Author(s): 佐藤幸治, ほか
  • Total Pages: 251
  • Publisher: 化学同人
• [Book] Insect Olfaction : Receptors, Signal Transduction, and Behavior, In Results and Problems in Cell Differentiation (2009)
  • Author(s): Sato K, Touhara K
  • Publisher: Springer, Berlin
• [Book] Results and Problems in Cell Differentiation (2009)
  • Author(s): 佐藤幸治
  • Publisher: Springer
• [Remarks]
  • URL: http://www.h.u-tokyo.ac.jp/press/press_archives/20100401.html
• [Remarks]
  • URL: http://www.a.u-tokyo.ac.jp/topics/2011/20110628-1.html
• [Remarks]
  • URL: http://www.a.u-tokyo.ac.jp/topics/2012/20120308-1.html
• [Remarks]
  • URL: http://www.a.u-tokyo.ac.jp/topics/2011/20110628-1.html
• [Remarks]
  • URL: http://www.a.u-tokyo.ac.jp/topics/2012/20120308-1.html