A nalys i s of biological effect o f clustered DNA damage induced by X-ray microbeam

• Project/Area Number: 21681006
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Central Research Institute of Electric Power Industry
• Principal Investigator: TOMITA Masanori (財)電力中央研究所, 原子力技術研究所, 主任研究員 (00360595)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000); Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2009: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: クラスターDNA損傷 / マイクロビーム / X線 / 放射線影響 / バイスタンダー応答

Research Abstract

DNA single-strand break(SSB) repair proteins were localized in the irradiated region of the cells with low doses of X-ray microbeam. W hen the irradiated doses became higher, DNA double-strand break(DSB) repair proteins were localized in the irradiate d region instead of SSB repair proteins. Repair efficiency of DSBs did not depend on the energy of X-ray. In addition, X-ray-induced bystander response depended on the tumor suppressor p53 status.

Research Products

• [Journal Article] Microbeam Studies of Soft X-ray Induced Bystander Cell Killing Using Microbeam X-ray Cell Irradiation System at CRIEPI (2012)
  • Author(s): Masanori Tomita, Katsumi Kobayashi, Munetoshi Maeda
  • Journal Title: Journal of Radiation Research Volume: 53 Issue: 3 Pages: 482-488
  • DOI: 10.1269/jrr.11055
  • NAID: 130001876861
  • ISSN: 0449-3060, 1349-9157
  • Peer Reviewed
• [Journal Article] Involvement of DNA-PK and ATM in Radiation- and Heat-induced DNA Damage Recognition and Apoptotic Cell Death (2010)
  • Author(s): Masanori Tomita
  • Journal Title: Journal of Radiation Research Volume: 51 Issue: 5 Pages: 493-501
  • DOI: 10.1269/jrr.10039
  • NAID: 10026644057
  • ISSN: 0449-3060, 1349-9157
  • Peer Reviewed
• [Journal Article] Bystander c ell k illing in n ormal h uman f ibroblasts is i nduced by s ynchrotron X-ray m icrobeams (2010)
  • Author(s): Masanori Tomita, Munetoshi Maeda, Hiroshi Maezawa, Noriko Usami, Katsumi Kobayashi
  • Journal Title: Radiation Research Volume: 173 Issue: 3 Pages: 380-385
  • DOI: 10.1667/rr1995.1
  • Peer Reviewed
• [Journal Article] Involvement of DNA-PK and ATM in radiation- and heat-induced DNA damage recognition and apoptotic cell death (2010)
  • Author(s): Masanori Tomita
  • Journal Title: Journal of Radiation Research Volume: VOL.51 Pages: 493-501
  • NAID: 10026644057
  • Peer Reviewed
• [Journal Article] Bystander Cell Killing in Normal Human Fibroblasts is Induced by Synchrotron X-ray Microbeams (2010)
  • Author(s): Masanori Tomita
  • Journal Title: Radiation Research VOL.173 Pages: 380-385
  • Peer Reviewed
• [Presentation] Development of Microbeam X-ray Cell Irradiation System at CRIEPI and its use in studying by stander response (2012)
  • Author(s): Masanori Tomita
  • Organizer: 10th International Workshop Microbeam Probes o f Cellular Radiation Response
  • Place of Presentation: Columbia University(米国)
  • Year and Date: 2012-03-15
• [Presentation] Development of Microbeam X-ray Cell Irradiation System at CRIEPI and Its Use in Studying Bystander Response (2012)
  • Author(s): Masanori Tomita
  • Organizer: 10th International Workshop Microbeam Probes of Cellular Radiation Response
  • Place of Presentation: Columbia University(米国)
  • Year and Date: 2012-03-15
• [Presentation] X線照射によって生じるバイスタンダー細胞死におけるp 53の役割 (2011)
  • Author(s): 冨田雅典
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス(兵庫県)
  • Year and Date: 2011-09-18
• [Presentation] 軟X線照射によって生じるバイスタンダー細胞死におけるp53の役割 (2011)
  • Author(s): 冨田雅典
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス(兵庫県)
  • Year and Date: 2011-09-18
• [Presentation] Microbeam studies of X-ray induced b ystander cell killing effect (2011)
  • Author(s): Masanori Tomita
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: The Palace of Culture and Science(ポーランド)
  • Year and Date: 2011-08-29
• [Presentation] Microbeam Studies of X-ray Induced Bystander Cell Killing Effect (2011)
  • Author(s): Masanori Tomita
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: The Palace of Culture and Science(ポーランド)
  • Year and Date: 2011-08-29
• [Presentation] X線マイクロビーム照射によって生じたDNA損傷に対するDNA修復タンパク質の応答 (2010)
  • Author(s): 冨田雅典
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2010-09-20
• [Presentation] Microbeam X-ray irradiation system at CRIEPI and its use in bystander response (2010)
  • Author(s): Masanori Tomita
  • Organizer: 9th International Microbeam Workshop
  • Place of Presentation: Technical University of Darmstadt(ドイツ)
  • Year and Date: 2010-07-15
• [Presentation] Microbeam X-ray irradiation system at CRIEPI and its use in bystander response (2010)
  • Author(s): Masanori Tomita
  • Organizer: 9th International Microbeam Workshop
  • Place of Presentation: Technical University of Darmstadt (ドイツ)
  • Year and Date: 2010-07-15
• [Presentation] X線マイクロビームを用いた放射線誘発DNA損傷修復機構の解析 (2009)
  • Author(s): 冨田雅典
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: アスティとくしま(徳島県)
  • Year and Date: 2009-11-01
• [Remarks]
  • URL: http://criepi.denken.or.jp/jp/ldrc/information/result/paperL.html
• [Remarks]
  • URL: http://http://criepi.denken.or.jp/jp/ldrc/information/result/paperL.html
• [Remarks]
  • URL: http://criepi.denken.or.jp/jp/ldrc/result/results.html
• [Remarks]
  • URL: http://criepi.denken.or.jp/jp/ldrc/result/results.html