Development of advanced wastewater treatment technology by deigning the microbial community

• Project/Area Number: 21681009
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Environmental technology/Environmental materials
• Research Institution: Osaka University
• Principal Investigator: SODA Satoshi 大阪大学, 工学研究科, 准教授 (30322176)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000); Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2009: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: 活性汚泥 / プラスミド / バイオオーグメンテーション / 廃水処理 / 微生物 / 遺伝子 / 微生物群集

Research Abstract

Application of bioaugmentation as a design method of the microbial community was proposed for advanced wastewater treatment. Distribution of bacteria and plasmids in activate sludge were investigated. Plasmid-mediated bioaugmentation was demonstrated for enhancing removal of 2,4-dichlorophenoxyacetic acid as a persistent organic pollutant by introducing bacteria harboring 2,4-D-degrading plasmid. Cell bioaugmentation was demonstrated for enhancing removal of selenium as a toxic metal by introducing a selenium-reducing bacterium.

Research Products

• [Journal Article] Plasmid-mediated bioaugmentation of sequencing batch reactors for enhancement of 2,4-dichlorophenoxyacetic acid removal in wastewater using plasmid pJP4. (2013)
  • Author(s): Tsutsui, H., Anami, Y., Matsuda, M., Hashimoto, K., Inoue, D., Sei, K., Soda, S., Ike, M.
  • Journal Title: Biodegradation Volume: 24 Pages: 343-352
• [Journal Article] Plasmid-mediated bioaugmentation of sequencing batch reactors for enhancement of 2,4-dichlorophenoxyacetic acid removal in wastewater using plasmid pJP4 (2013)
  • Author(s): Tsutsui, H., Anami, Y., Matsuda, M., Hashimoto, K., Inoue, D., Sei, K., Soda, S., and Ike, M.
  • Journal Title: Biodegradation Volume: 24 Issue: 3 Pages: 343-352
  • DOI: 10.1007/s10532-012-9591-4
  • Peer Reviewed
• [Journal Article] ジーン バイオオーグメンテーションによる活性汚泥リアクターの2,4-D除去能の向上 (2012)
  • Author(s): 惣田訓、穴見泰崇、筒井裕文、橋本くるみ、松田真佐美、井上大介、清和成、池道彦
  • Journal Title: 土木学会論文集G (環境) Volume: 68巻
• [Journal Article] Biotreatment of selenium refinery wastewater using pilot-scale granular sludge and swim-bed bioreactors augmented with a selenium-reducing bacteriumPseudomonas stutzeri NT-I. (2012)
  • Author(s): Soda, S., Takahashi, H., Kagami, T., Miyake, M., Notaguchi, E., Sei, K,Iwasaki, N., and Ike, M.
  • Journal Title: Japanese Journal Water Treatment Biology Volume: 48 Pages: 63-71
  • NAID: 10030809041
• [Journal Article] 活性汚泥内の抗生物質耐性細菌および重金属耐性細菌の保有するプラスミドの特徴づけ (2012)
  • Author(s): 橋本くるみ、井上大介、惣田訓、池道彦
  • Journal Title: 下水道協会誌論文集 Volume: 49/594 Pages: 104-111
  • NAID: 10030145147
• [Journal Article] ジーン バイオオーグメンテーションによる活性汚泥リアクターの2,4-D除去能の向上 (2012)
  • Author(s): 惣田訓，穴見泰崇，筒井裕文，橋本くるみ，松田真佐美，井上大介，清和成，池道彦
  • Journal Title: 土木学会論文集G (環境) Volume: 68
  • Peer Reviewed
• [Journal Article] 活性汚泥内の抗生物質耐性細菌および重金属耐性細菌の保有するプラスミドの特徴づけ (2012)
  • Author(s): 橋本くるみ，井上大介，惣田訓，池道彦
  • Journal Title: 下水道協会誌論文集 Volume: 49 Pages: 104-111
  • NAID: 10030145147
  • Peer Reviewed
• [Journal Article] Impacts of gene bioaugmentation with pJP4-harboring bacteria of 2,4-D-contaminated soil slurry on the indigenous microbial community (2012)
  • Author(s): Daisuke Inoue, Yuji Yamazaki, Hirofumi Tsutsui, Kazunari Sei, Satoshi Soda, Masanori Fujita, Michihiko Ike
  • Journal Title: Biodegradation Volume: VOL.23 Issue: 2 Pages: 263-276
  • DOI: 10.1007/s10532-011-9505-x
  • Peer Reviewed
• [Journal Article] Impacts of gene bioaugmentation with pJP4-harboring bacteria of 2,4-D-contaminated soil slurry on the indigenous microbial community. (2011)
  • Author(s): Inoue, D., Yamazaki, Y., Tsutsui, H., Sei, K., Soda, S., Fujita, M., Ike M.
  • Journal Title: Biodegradation Volume: 23 Pages: 263-276
• [Journal Article] Transfer of plasmid pJP4 from Escherichia coliand Pseudomonas putidato bacteria in activated sludge developed under different sludge retention times. (2010)
  • Author(s): Tsutsui, H., Anami, Y., Matsuda, M., Inoue, D., Sei, K., Soda, S., and Ike, M.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 110 Pages: 684-689
• [Journal Article] Transfer of plasmid pJP4 from Escherichia coli and Pseudomonas putida to bacteria in activated sludge developed under different sludge retention times. (2010)
  • Author(s): Tsutsui, H., Anami, Y., Matsuda, M., Inoue, D., Sei, K., Soda, S., Ike, M.
  • Journal Title: Journal of.Bioscience and Bioengineering Volume: 110 Pages: 684-689
  • NAID: 110008143504
  • Peer Reviewed
• [Journal Article] Transfer of plasmid pJP4 from Escherichia colito activated sludge bacteria by filter mating (2009)
  • Author(s): Tsutsui, H., Soda, S., Takeda, S., Ohtsuki, H., Inoue, D., Sei, K., and Ike, M.
  • Journal Title: Japanese Journal of Water Treatment Biology Volume: 45 Pages: 185-191
• [Journal Article] Transfer of plasmid pJP4 from Escherichia coli to activated sludge bacteria by filter mating (2009)
  • Author(s): Hirofumi Tsutsui, Satoshi Soda, Satoshi Takeda, Hidetaka Ohtsuki, Daisuke Inoue, Kazunari Sei, Michihiko Ike
  • Journal Title: Japanese Journal of Water Treatment Biology VOL.45 Pages: 185-191
  • NAID: 120004837347
  • Peer Reviewed
• [Presentation] ジーン バイオオーグメンテーションによる活性汚泥リアクターの2,4-D除去能の向上 (2012)
  • Author(s): 惣田訓，穴見泰崇，筒井裕文，橋本くるみ，松田真佐美，井上大介，清和成，池道彦
  • Organizer: 土木学会環境工学フォーラム
  • Place of Presentation: 京都大学百周年会館
• [Presentation] Isolation and characterization of plasmids harbored by antibiotic- and metal-resistant bacteria in activated sludge. (2011)
  • Author(s): Hashimoto, K., Tsutsui, H., Inoue, D., Sei, K., Soda, S., Ike, M.
  • Organizer: The 4th International Water Association -Asia-Pacific Region Conference & Exhibition
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2011-10-04
• [Presentation] Plasmid-mediated bioaugmentation of sequencing batch reactor for enhancement of 2,4-dichlorophenoxyacetic acid removal in wastewater using plasmid pJP4 (2011)
  • Author(s): Tsutsui, H., Anami, Y., Matsuda, M., Hashimoto, K., Inoue, D., Sei, K., Soda, S., and Ike, M.
  • Organizer: The 4th International Water Association -Asia-Pacific Region Conference & Exhibition.
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2011-10-04
• [Presentation] Biotreatment of selenium refinery wastewater using pilot-scale UASB and swim-bed bioreactors augmented with a selenium-reducing bacterium Pseudomonas stutzeriNT-I. . (2011)
  • Author(s): Soda, S., Takahashi, H., Kagami, T., Miyake, M., Notaguchi, E., Sei, K., Iwasaki, N., and Ike, M.
  • Organizer: The 4th International Water Association -Asia-Pacific Region Conference & Exhibition
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2011-10-04
• [Presentation] Isolation and characterization of plasmids harbored by antibiotic- and metal-resistant bacteria in activated sludge (2011)
  • Author(s): Hashimoto, K., Tsutsui, H., Inoue, D., Sei, K., Soda, S., Ike, M.
  • Organizer: The 4th International Water Association-Asia-Pacific Region Conference & Exhibition
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2011-10-04
• [Presentation] Plasmid-mediated bioaugmentation of sequencing batch reactor for enhancement of 2,4-dichlorophenoxyacetic acid removal in wastewater using plasmid pJP4 (2011)
  • Author(s): Tsutsui, H., Anami, Y., Matsuda, M., Hashimoto, K., Inoue, D., Sei, K., Soda, S., Ike, M.
  • Organizer: The 4th International Water Association-Asia-Pacific Region Conference & Exhibition
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2011-10-04
• [Presentation] 池道彦活性汚泥内の重金属と抗生物質耐性細菌の有するプラスミドの特徴づけ (2011)
  • Author(s): 橋本くるみ、筒井裕文、井上大介、清和成、惣田訓
  • Organizer: 第45回日本水環境学会年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-03-18
• [Presentation] 活性汚泥内の重金属と抗生物質耐性細菌の有するプラスミドの特徴づけ (2011)
  • Author(s): 橋本くるみ, 筒井裕文, 井上大介, 清和成, 惣田訓, 池道彦
  • Organizer: 第45回日本水環境学会年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-03-18
• [Presentation] 実下水処理場内の微生物群集の構造と炭素源資化能の解析 (2010)
  • Author(s): 井上大介、松田真佐美、〓俊琴、筒井裕文、清和成、惣田訓、池道彦
  • Organizer: 日本水処理生物学会第47回大会
  • Place of Presentation: 筑波大学会館
  • Year and Date: 2010-11-19
• [Presentation] 活性汚泥内に存在する抗生物質・重金属耐性細菌の有するプラスミドの特徴づけ (2010)
  • Author(s): 橋本くるみ、筒井裕文、池道彦、井上大介、清和成、惣田訓、池道彦
  • Organizer: 第47回日本下水道研究発表会
  • Place of Presentation: ポートメッセなごや
  • Year and Date: 2010-07-28
• [Presentation] 活性汚泥内に存在する抗生物質・重金属耐性細菌の有するプラスミドの特徴づけ (2010)
  • Author(s): 橋本くるみ, 筒井裕文, 池道彦, 井上大介, 清和成, 惣田訓
  • Organizer: 第47回日本下水道研究発表会
  • Place of Presentation: ポートメッセなごや(名古屋市)
  • Year and Date: 2010-07-28
• [Presentation] プラスミドオーグメンテーションによる活性汚泥の2,4-D分解能強化の検討 (2010)
  • Author(s): 筒井博文, 惣田訓, 池道彦, 穴見泰崇, 松田真佐美, 井上大介, 清和成
  • Organizer: 第47回日本下水道研究発表会
  • Place of Presentation: ポートメッセなごや(名古屋市)
  • Year and Date: 2010-07-28
• [Presentation] プラスミド・オーグメンテーションによる活性汚泥プロセスの2 (2009)
  • Author(s): 惣田訓、穴見泰崇、筒井裕文、松田真佐美、井上大介、清和成、池道彦
  • Organizer: 4-D分解能の強化日本水処理生物学会
  • Place of Presentation: 高知市文化プラザかるぽーと
  • Year and Date: 2009-11-13
• [Presentation] プラスミド・オーグメンテーションによる活性汚泥プロセスの2、4-D分解能の強化 (2009)
  • Author(s): 惣田訓、穴見泰崇、筒井裕文、松田真佐美、井上大介、清和成、池道彦
  • Organizer: 日本水処理生物学会
  • Place of Presentation: 高知市文化プラザかるぽーと(高知市)
  • Year and Date: 2009-11-13