|Budget Amount *help
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
We have successfully transplanted a plant specific degradation pathway, that is controlled by the plant hormone auxin, to non-plant cells. This new technology, termed the auxin-inducible degron (AID) system, allows us to deplete a protein of interest in an auxin dependent manner. We showed that AID works in budding and fission yeasts, as well as in chicken DT40, mouse and human cells, and the results were published. We also applied the AID technology for the characterization of proteins in budding yeast and the results were published. Moreover, we successfully made an improved version of AID and applied for a patent.