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Differentiation response of stem cells in noisy induction environments

Research Project

Project/Area Number 21684024
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Biophysics/Chemical physics
Research InstitutionThe University of Tokyo

Principal Investigator

WAKAMOTO Yuichi  東京大学, 大学院・総合文化研究科, 准教授 (30517884)

Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2009: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Keywords生物物理 / 1細胞計測 / 幹細胞 / 適応度 / 分化応答 / ES細胞 / 分化 / 応答特性
Research Abstract

Stem cells can differentiate into different cell types according to external cues andintrinsically generated information. For understanding the logic of stem cell differentiation, an experimental tool that allows the measurement of state transition of stem cells under controlled environmental conditions is required in addition to conventional population-level measurements. In this research project, we developeda new single-cell analysis device and applied it to the study on differentiation response of mouse ES cells at the single-cell level.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (34 results)

All 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 3 results) Presentation (12 results) (of which Invited: 3 results) Book (2 results) Remarks (6 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] 細胞計測で明らかになる集団の増殖と適応2013

    • Author(s)
      野添嵩、橋本幹弘、若本祐一、
    • Journal Title

      実験医学

      Volume: 31(8) Pages: 1209-1216

    • Related Report
      2012 Final Research Report
  • [Journal Article] ネガティブフィードバックのトレードオフを回避するウイルスの発現制御回路2013

    • Author(s)
      若本祐一
    • Journal Title

      実験医学

      Volume: 31(6) Pages: 893-894

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] DynamicPersistence of Antibiotic-StressedMycobacteria2013

    • Author(s)
      Wakamoto, Y., Dhar, N., Chait, R.,Schneider, K., Signorino-Gelo, F.,Leibler, S., McKinney, J. D.
    • Journal Title

      Science

      Volume: 339(6115) Issue: 6115 Pages: 91-95

    • DOI

      10.1126/science.1229858

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 1細胞計測で明らかになる集団の増殖と適応2013

    • Author(s)
      野添 嵩
    • Journal Title

      実験医学

      Volume: 31 Pages: 1209-1216

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 生細胞1個の質量変化を測る2012

    • Author(s)
      若本祐一
    • Journal Title

      実験医学

      Volume: 30(18) Pages: 2950-2951

    • Related Report
      2012 Final Research Report
  • [Journal Article] 抗生物質に対する耐性菌進化の予測は可能か?2012

    • Author(s)
      若本祐一
    • Journal Title

      実験医学

      Volume: 30(8) Pages: 1294-1295

    • Related Report
      2012 Final Research Report
  • [Journal Article] Optimal lineageprinciple for age-structured populations2012

    • Author(s)
      Wakamoto, Y., Grosberg, A. Y.,Kussell, E.
    • Journal Title

      Evolution

      Volume: 66(1) Issue: 1 Pages: 115-134

    • DOI

      10.1111/j.1558-5646.2011.01418.x

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 生細胞1個の質量変化を測る2012

    • Author(s)
      若本祐一
    • Journal Title

      実験医学

      Volume: 30 Pages: 2950-2951

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 抗生物質に対する耐性菌進化の予測は可能か?2012

    • Author(s)
      若本祐一
    • Journal Title

      実験医学

      Volume: 30 Pages: 1294-1295

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 確率的表現型変化によるがん細胞構成比の安定性2011

    • Author(s)
      若本祐一
    • Journal Title

      実験医学

      Volume: 29(18) Pages: 2980-2981

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Journal Article] Multitude of morphologicaldynamics of giant multilamellar vesicles in regulated nonequilibriumenvironments2011

    • Author(s)
      Tomita, T., Sugawara, T., Wakamoto,Y.
    • Journal Title

      Langmuir

      Volume: 27(16) Issue: 16 Pages: 10106-10112

    • DOI

      10.1021/la2018456

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 成長ゆらぎに依存する細胞集団と歴史統計量の性質2013

    • Author(s)
      若本祐一
    • Organizer
      2012年度国立遺伝学研究所研究会「単細胞システムの構築とその維持機構の研究」
    • Place of Presentation
      国立遺伝学研究所(三島)
    • Related Report
      2012 Final Research Report
  • [Presentation] 成長ゆらぎに依存する細胞集団と歴史統計量の性質2013

    • Author(s)
      若本 祐一
    • Organizer
      2012年度国立遺伝学研究所研究会「単細胞システムの構築とその維持機構の研究」
    • Place of Presentation
      国立遺伝学研究所(三島)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Bacterial persistence based on epigenetically correlated stochastic gene expression2012

    • Author(s)
      Wakamoto, Y.
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎(招待講演)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 分化様のふるまいを示す人工遺伝子ネットワークの構築2012

    • Author(s)
      小野すみれ、大倉玲子、若本祐一
    • Organizer
      定量生物の会第5回年会
    • Place of Presentation
      東京大学生産技術研究所(東京)
    • Related Report
      2012 Final Research Report
  • [Presentation] Inconsistencybetweenpopulationandsinglecellgrowthratesrevealedbydynamicscytometer2012

    • Author(s)
      YuichiWakamoto
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(名古屋)
    • Related Report
      2012 Final Research Report
  • [Presentation] Syntheticbiologyofstemcells:creatingartificialgenenetworkof"differentiation2012

    • Author(s)
      SumireOno,ReikoOkura,YuichiWakamoto
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(名古屋)
    • Related Report
      2012 Final Research Report
  • [Presentation] 幹細胞様のふるまいを示す人工遺伝子ネットワークの構築2012

    • Author(s)
      小野すみれ、若本祐一
    • Organizer
      第9回21世紀大腸菌研究会
    • Place of Presentation
      長浜ロイヤルホテル(長浜)
    • Related Report
      2012 Final Research Report
  • [Presentation] バクテリアのクローン増殖の背景にある現象論的ルール2012

    • Author(s)
      若本 祐一
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Inconsistency between population and single cell growth rates revealed by dynamics cytometer2012

    • Author(s)
      Yuichi Wakamoto
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(名古屋)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Natural selection based on stochastic gene expression and generality of phenotypic adaptation2011

    • Author(s)
      Wakamoto, Y.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜(招待講演)
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Bacterial persistence based on epigenetically correlated gene expression2011

    • Author(s)
      Wakamoto, Y.
    • Organizer
      5th International Conference on Analysis of Microbial Cells at the Sinele Cell Level
    • Place of Presentation
      Carry-Le-Rouet, France
    • Year and Date
      2011-11-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Single-cell measurement toolbox for studying evolution of restriction-modification systems2011

    • Author(s)
      Wakamoto, Y.
    • Organizer
      The 11th HFSP Awardees Meeting
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2011-06-06
    • Related Report
      2011 Annual Research Report
  • [Book] Controlling Cell Migration with Micropatterns2012

    • Author(s)
      Toyota, T., Wakamoto, Y., Hayashi, K.Ohnuma,K.
    • Publisher
      Innovations in Biotechnology, Dr. Eddy C. Agbo (Ed.), InTech,
    • URL

      http://www.intechopen.com/books/innovations-in-biotechnology/controlling-cell-migration-with-micropatterns

    • Related Report
      2012 Final Research Report
  • [Book] Controlling Cell Migration with Micropattems, In : Innovations in Biotechnology2012

    • Author(s)
      Toyota, T., Wakamoto, Y., Hayashi, K., Ohnuma, K.
    • Total Pages
      474
    • Publisher
      InTech
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/wakamoto-lab/index_j.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 若本研究室ホームページ

    • URL

      http://park.itc.u-tokyo.ac.jp/wakamoto-lab/index_j.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] ReaD & Researchmap

    • URL

      http://researchmap.jp/ywakamoto/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/wakamoto-lab/index_j.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/wakamoto-lab/index_j.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/wakamoto-lab/indexj.html

    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞培養装置、細胞培養方法、および細胞培養観察方法2012

    • Inventor(s)
      若本祐一 、橋本幹弘
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構
    • Filing Date
      2012-07-13
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 胞培養装置、細胞培養方法、および細胞培養観察方法2011

    • Inventor(s)
      若本祐一 、橋本幹弘
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構
    • Industrial Property Number
      2011-156767
    • Filing Date
      2011-07-15
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 細胞培養装置、細胞培養方法、および細胞培養観察方法2011

    • Inventor(s)
      若本祐一、橋本幹弘
    • Industrial Property Rights Holder
      科学技術振興機構(100%)
    • Industrial Property Number
      2011-156767
    • Filing Date
      2011-07-15
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2019-07-29  

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