| Budget Amount *help |
¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2011: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2009: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
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| Research Abstract |
I developed a compound-specific isotope method for measuring the stable nitrogen, carbon, and hydrogen isotopic compositions of amino acids. The results show(1) nitrogen isotopic composition can be determined by pivaloyl/isopropyl ester derivatization, with better than±0.5‰and the minimum sample amount of 0.5 nmol N,(2) carbon isotopic composition can be determined by ethyl ester derivatization, with better than±0.5‰and the minimum sample amount of 0.1 nmol N, and hydrogen isotopic composition can be determined by pivaloyl/isopropyl ester derivatization, with better than±50-100‰and the minimum sample amount of 0.5 nmol N. However, the carbon isotope analysis is only available for standard solution of amino acids but not for natural samples, at this moment.
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