Construction of biocatalysts based on substrate misrecognition of enzymes
| Project/Area Number |
21685018
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Nagoya University |
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Principal Investigator |
SHOJI Osami 名古屋大学, 理学研究科, 助教 (90379587)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2009: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Keywords | 酵素化学 / 蛋白質 / 酵素反応 / バイオテクノロジー / 生体機能利用 / シトクロムP450 / 疑似基質 / 過酸化水素 / ガス状アルカン / P450 |
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| Research Abstract |
We have demonstrated that the substrate specificity of cytochrome P450s can be altered by inducing their substrate misrecognition using a decoy molecule that has a structural similarity to those of natural substrate. Accordingly, we have succeeded in constructing a novel reaction system that catalyzes oxidation reactions of non-natural substrates without any mutagenesis. Notably, our reaction system can alter the enantioselectivity of products just by changing the structure of the decoy molecules.
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Report
(4 results)
Research Products
(49 results)
