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Development of fusion molecular imaging technique based on functional molecular design

Research Project

Project/Area Number 21685019
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Chemistry related to living body
Research InstitutionOsaka University

Principal Investigator

MIZUKAMI Shin  大阪大学, 大学院・工学研究科, 准教授 (30420433)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2010: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2009: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Keywords蛍光プローブ / MRI / ラベル化 / イメージング / β-ラクタマーゼ / βラクタマーゼ / タンパク質ラベル / 量子ドット
Research Abstract

Several elemental technologies for next-generation molecular imaging methods were developed by integrating chemical principle-based functional probe design, protein engineering, and latest instrumental analyses. Specifically, three technologies, which are^19F MRI detection system to visualize living cellular gene expression, novel protein labeling technology, and time-resolved photoluminescence imaging technology for proteins, were developed.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (90 results)

All 2012 2011 2010 2009 Other

All Journal Article (43 results) (of which Peer Reviewed: 42 results) Presentation (34 results) Book (5 results) Remarks (4 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 2 results)

  • [Journal Article] No-Wash Protein Labeling with Designed Fluorogenic Probes and Application to Real-Time Pulse-Chase Analysis2012

    • Author(s)
      Shin Mizukami, Shuji Watanabe, Yuri Akimoto, Kazuya Kikuchi
    • Journal Title

      J. Am. Chem. Soc

      Volume: 134 Pages: 1623-1629

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Switchable MRI Contrast Agents Based on Morphological Changes of pH-Responsive Polymers2012

    • Author(s)
      Satoshi Okada, Shin Mizukami, Kazuya Kikuchi
    • Journal Title

      Bioorg. Med. Chem

      Volume: 20 Pages: 769-774

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A New Approach for Fluorogenic Protein Labeling via Photoinduced Electron Transfer Based BL-tag Protein Labeling Method2012

    • Author(s)
      Kalyan K. Sadhu, Shin Mizukami, Carolyn R. Lanam, and Kazuya Kikuchi
    • Journal Title

      Chem. Asian J.

      Volume: 7 Pages: 272-276

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 生体反応を可視化する19F MRIプローブ開発2012

    • Author(s)
      水上進・菊地和也
    • Journal Title

      生物物理

      Volume: 52 Pages: 24-25

    • NAID

      10030038125

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A New Approach for Fluorogenic Protein Labeling via Photoinduced Electron Transfer Based BL-tag Protein Labeling Method2012

    • Author(s)
      Kalyan K.Sadhu, Shin Mizukami, Carolyn R.Lanam, Kazuya Kikuchi
    • Journal Title

      Chem.Asian J.

      Volume: 7 Issue: 2 Pages: 272-276

    • DOI

      10.1002/asia.201100647

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Switchable MRI Contrast Agents Based on Morphological Changes of pH-responsive Polymers2012

    • Author(s)
      S. Okada, S. Mizukami & K. Kikuchi
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 20 Issue: 2 Pages: 769-774

    • DOI

      10.1016/j.bmc.2011.12.005

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] No-Wash Protein Labeling with Designed Fluorogenic Probes and Application to Real-Time Pulse-Chase Analysis2012

    • Author(s)
      S. Mizukami, S. Watanabe, Y. Akimoto & K. Kikuchi
    • Journal Title

      J. Am. Chem. Soc

      Volume: 134 Issue: 3 Pages: 1623-1629

    • DOI

      10.1021/ja208290f

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of 19F MRI Probes that Visualize Biological Reactions2012

    • Author(s)
      水上進・菊地和也
    • Journal Title

      Seibutsu Butsuri

      Volume: 52 Issue: 1 Pages: 024-025

    • DOI

      10.2142/biophys.52.024

    • NAID

      10030038125

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Molecular Imaging Tools to Investigate Protein Functions by Chemical Probe Design2011

    • Author(s)
      Shin Mizukami
    • Journal Title

      Chem. Pharm. Bull

      Volume: 59 Pages: 1435-1446

    • NAID

      130001852115

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Covalent Protein Labeling with a Lanthanide Complex and its Application to Photoluminescence Lifetime-based Multicolor Bioimaging2011

    • Author(s)
      Shin Mizukami, Taku Yamamoto, Akimasa Yoshimura, Shuji Watanabe, and Kazuya Kikuchi
    • Journal Title

      Angew. Chem. Int. Ed

      Volume: 50 Pages: 8750-8752

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular Protein Labeling with Prodrug-Like Probes Using a Mutant.-Lactamase Tag2011

    • Author(s)
      Shuji Watanabe, Shin Mizukami, Yuri Akimoto, Yuichiro Hori, and Kazuya Kikuchi
    • Journal Title

      Chem. Eur. J

      Volume: 17 Pages: 8342-8349

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 19F MRI Detection of.-Galactosidase Activity for Imaging of Gene Expression2011

    • Author(s)
      Shin Mizukami, Hisashi Matsushita, Rika Takikawa, Fuminori Sugihara, Masahiro Shirakawa, and Kazuya Kikuchi
    • Journal Title

      Chem. Sci

      Volume: 2 Pages: 1151-1155

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sequential Ordering among Multicolor Fluorophores for Protein Labeling Facility via Aggregation-Elimination Based.-Lactam Probes2011

    • Author(s)
      Kalyan K. Sadhu, Shin Mizukami, Shuji Watanabe, and Kazuya Kikuchi
    • Journal Title

      Mol. BioSyst

      Volume: 7 Pages: 1766-1772

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cell-Surface Protein Labeling with Luminescent Nanoparticles through Biotinylation by Using Mutant.-Lactamase-Tag Technology2011

    • Author(s)
      Akimasa Yoshimura, Shin Mizukami, Yuichiro Hori, Shuji Watanabe, and Kazuya Kikuchi
    • Journal Title

      ChemBioChem

      Volume: 12 Pages: 1031-1034

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Switching Modulation for Protein Labeling with Activatable Fluorescent Probe2011

    • Author(s)
      Kalyan K. Sadhu, Shin Mizukami, Yuichiro Hori, and Kazuya Kikuchi
    • Journal Title

      ChemBioChem

      Volume: 12 Pages: 1299-1308

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] β-ラクタマーゼを用いた蛋白質可視化技術の開発2011

    • Author(s)
      水上進・渡辺修司・吉村彰真・菊地和也
    • Journal Title

      JSMI report

      Volume: 4 Pages: 19-21

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular Protein Labeling with Prodrug-Like Probes Using a Mutant β-Lactamase Tag2011

    • Author(s)
      Shuji Watanabe, Shin Mizukami, Yuri Akimoto, Yuichiro Hori, Kazuya Kikuchi
    • Journal Title

      Chem. Eur. J.

      Volume: 17 Issue: 30 Pages: 8342-8349

    • DOI

      10.1002/chem.201100973

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Covalent Protein Labeling with a Lanthanide Complex and its Application to Photoluminescence Lifetimebased Multicolor Bioimaging2011

    • Author(s)
      S. Mizukami, T. Yamamoto, A. Yoshimura, S. Watanabe & K. Kikuchi
    • Journal Title

      Angew. Chem. Int. Ed

      Volume: 50 Issue: 37 Pages: 8750-8752

    • DOI

      10.1002/anie.201103775

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Molecular Imaging Tools to Investigate Protein Functions by Chemical Probe Design2011

    • Author(s)
      Shin Mizukami
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 59 Issue: 12 Pages: 1435-1446

    • DOI

      10.1248/cpb.59.1435

    • NAID

      130001852115

    • ISSN
      0009-2363, 1347-5223
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell-Surface Protein Labeling with Luminescent Nanoparticles through Biotinylation by Using Mutant β-Lactamase-Tag Technology2011

    • Author(s)
      Akimasa Yoshimura, Shin Mizukami, Yuichiro Hori, Shuji Watanabe, Kazuya Kikuchi
    • Journal Title

      ChemBioChem

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequential Ordering among Multicolor Fluorophores for Protein Labeling Facility Aggregation-Elimination Based β-Lactam Probes2011

    • Author(s)
      Kalyan K.Sadhu, Shin Mizukami, Shuji Watanabe, Kazuya Kikuchi
    • Journal Title

      Mol.Biosys.

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ^<19>F MRI Detection of β-Galactosidase Activity for Imaging of Gene Expression2011

    • Author(s)
      Shin Mizukami, Hisashi Matsushita, Rika Takikawa, Fuminori Sugihara, Masahiro Shirakawa, Kazuya Kikuchi
    • Journal Title

      Chem.Sci.

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multicolor Protein Labeling in Living Cells Using Mutant.-lactamase-tag Technology2010

    • Author(s)
      Shuji Watanabe, Shin Mizukami, Yuichiro Hori, and Kazuya Kikuchi
    • Journal Title

      Bioconjug. Chem

      Volume: 21 Pages: 2320-2326

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Turn-on Fluorescent Switch Involving Aggregation and Elimination Processes forβ-Lactamase-Tag2010

    • Author(s)
      Kalyan K. Sadhu, Shin Mizukami, Shuji Watanabe, and Kazuya Kikuchi
    • Journal Title

      Chem. Commun

      Volume: 46 Pages: 7403-7405

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photocontrolled Compound Release System Using Caged Antimicrobial Peptide2010

    • Author(s)
      Shin Mizukami, Mariko Hosoda, Takafumi Satake, Satoshi Okada, Yuichiro Hori, Toshiaki Furuta, and Kazuya Kikuchi
    • Journal Title

      J. Am. Chem. Soc

      Volume: 132 Pages: 9524-9525

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of a Stimuli-responsive Polymer to the Development of Novel MRI Probes2010

    • Author(s)
      Satoshi Okada, Shin Mizukami, and Kazuya Kikuchi
    • Journal Title

      ChemBioChem

      Volume: 11 Pages: 785-787

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 化学プローブのデザイン・合成によるタンパク質機能の可視化ツール2010

    • Author(s)
      水上進・菊地和也
    • Journal Title

      生化学

      Volume: 82 Pages: 21-29

    • NAID

      10026300192

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photocontrolled Compound Release System Using Caged Antimicrobial Peptide2010

    • Author(s)
      Shin Mizukami, Mariko Hosoda, Takafumi Satake, Satoshi Okada, Yuichiro Hori, Toshiaki Furuta, Kazuya Kikuchi
    • Journal Title

      J.Am.Chem.Soc.

      Volume: 132 Pages: 9524-9525

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Turn-on Fluorescent Switch Involving Aggregation and Elimination Processes for β-Lactamase-Tag2010

    • Author(s)
      Kalyan K.Sadhu, Shin Mizukami, Shuji Watanabe, Kazuya Kikuchi
    • Journal Title

      Chem.Commun.

      Volume: 46 Pages: 7403-7405

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multicolor Protein Labeling in Living Cells Using Mutant β-lactamase-tag Technology2010

    • Author(s)
      Shuji Watanabe, Shin Mizukami, Yuichiro Hori, Kazuya Kikuchi
    • Journal Title

      Bioconjug.Chem.

      Volume: 21 Pages: 2320-2326

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 金属イオンプローブを用いた生体機能イメージング2010

    • Author(s)
      水上進・松下尚嗣・菊地和也
    • Journal Title

      化学フロンティア-生命現象を理解する分子ツール

      Pages: 15-24

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Application of Stimuli-responsive Polymer for Development of Novel MRI Probes2010

    • Author(s)
      Satoshi Okada, Shin Mizukami, Kazuya Kikuchi
    • Journal Title

      ChemBioChem (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 化学プローブのデザイン・合成によるタンパク質機能の可視化ツール2010

    • Author(s)
      水上進・菊地和也
    • Journal Title

      生化学 82

      Pages: 21-29

    • NAID

      10026300192

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design and Synthesis of Coumarin-based Zn2+ Probes for Ratiometric Fluorescence Imaging2009

    • Author(s)
      Shin Mizukami, Satoshi Okada, Satoshi Kimura, and Kazuya Kikuchi
    • Journal Title

      Inorg. Chem

      Volume: 48 Pages: 7630-7638

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of Ratiometric Fluorescent Probes for Phosphatases by Using a pKa-Switching Mechanism2009

    • Author(s)
      Shin Mizukami, Shuji Watanabe, and Kazuya Kikuchi
    • Journal Title

      ChemBioChem

      Volume: 10 Pages: 1465-1468

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Covalent Protein Labeling Based on Noncatalyticβ-Lactamase and a Designed FRET Substrate2009

    • Author(s)
      Shin Mizukami, Shuji Watanabe, Yuichiro Hori, and Kazuya Kikuchi
    • Journal Title

      J. Am. Chem. Soc

      Volume: 131 Pages: 5016-5017

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] in vivoイメージングを目指した可視化プローブ開発2009

    • Author(s)
      菊地和也・水上進
    • Journal Title

      ぶんせき

      Volume: 4 Pages: 200-202

    • NAID

      10025216425

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Covalent Protein Labeling Based on Noncatalytic ss-Lactamase and a Designed FRET Substrate2009

    • Author(s)
      Shin Mizukami, Shuji Watanabe, Yuichiro Hori, Kazuya Kikuchi
    • Journal Title

      J.Am.Chem.Soc. 131

      Pages: 5016-5017

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Ratiometric Fluorescent Probes for Phosphatases Using pK_a Switching Mechanism2009

    • Author(s)
      Shin Mizukami, Shuji Watanabe, Kazuya Kikuchi
    • Journal Title

      ChemBioChem 10

      Pages: 1465-1468

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anion Sensor-Based Ratiometric Peptide Probe for Protein Kinase Activity2009

    • Author(s)
      Kazuya Kikuchi, Shigeki Hashimoto, Shin Mizukami, Tetsuo Nagano
    • Journal Title

      Org.Lett. 11

      Pages: 2732-2735

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design and Synthesis of Coumarin-based Zn^<2+> Probes for Ratiometric Fluorescence Imaging2009

    • Author(s)
      Shin Mizukami, Satoshi Okada, Satoshi Kimura, Kazuya Kikuchi
    • Journal Title

      Inorg.Chem. 48

      Pages: 7630-7638

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoactive Yellow Protein-based Protein Labeling System with Turn-on Fluorescence Intensity2009

    • Author(s)
      Yuichiro Hori, Hideki Ueno, Shin Mizukami, Kazuya Kikuchi
    • Journal Title

      J.Am.Chem.Soc. 131

      Pages: 16610-16611

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] in vivoイメージングを目指した可視化プローブ開発2009

    • Author(s)
      菊地和也・水上進
    • Journal Title

      ぶんせき 4

      Pages: 200-202

    • NAID

      10025216425

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] ナノプローブ設計に基づく生体機能解析技術の開発2012

    • Author(s)
      水上進
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2012-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] ナノプローブ設計に基づく生体機能解析技術の開発2012

    • Author(s)
      水上進
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌)(招待講演)
    • Year and Date
      2012-03-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 精密プローブ設計に基づくバイオイメージング技術の開発2012

    • Author(s)
      水上進
    • Organizer
      理研ものつくりシンポジウム
    • Place of Presentation
      理化学研究所(和光)
    • Year and Date
      2012-03-08
    • Related Report
      2011 Final Research Report
  • [Presentation] 精密プローブ設計に基づくバイオイメージング技術の開発2012

    • Author(s)
      水上進
    • Organizer
      理研ものつくりシンポジウム
    • Place of Presentation
      理化学研究所(和光)(招待講演)
    • Year and Date
      2012-03-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] ナノとバイオを繋ぐ分子設計2011

    • Author(s)
      水上進
    • Organizer
      フロンティア生命化学研究会
    • Place of Presentation
      ラフォーレ南紀白浜(白浜町)
    • Year and Date
      2011-12-03
    • Related Report
      2011 Final Research Report
  • [Presentation] ナノとバイオを繋ぐ分子設計2011

    • Author(s)
      水上進
    • Organizer
      フロンティア生命化学研究会
    • Place of Presentation
      ラフォーレ南紀白浜(白浜町)(招待講演)
    • Year and Date
      2011-12-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Fast Fluorogenic Protein Labeling with Designed FRET Probes and Application to Real-Time Pulse-Chase Analysis2011

    • Author(s)
      Shin Mizukami, Shuji Watanabe, Yuri Akimoto, Kazuya Kikuchi
    • Organizer
      Imaging 2020
    • Place of Presentation
      Jackson Lake Lodge(WY, USA)
    • Year and Date
      2011-09-18
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞内蛋白質の蛍光ラベル化法の開発2011

    • Author(s)
      水上進.秋元悠里.菊地和也
    • Organizer
      バイオ関連化学合同シンポジウム
    • Place of Presentation
      つくば国際会議場(つくば)
    • Year and Date
      2011-09-12
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Photocontrolled compound release using caged antimicrobial peptide2011

    • Author(s)
      Shin Mizukami, Kengo Matsumoto, Kazuya Kikuchi
    • Organizer
      Gordon Research Conference, Bioorganic Chemistr
    • Place of Presentation
      Procter Academy(NH, USA)
    • Year and Date
      2011-06-12
    • Related Report
      2011 Final Research Report
  • [Presentation] 革新的分子イメージングへの応用を目的とした生体機能解析技術の開発2011

    • Author(s)
      水上進
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡県コンベンションアーツセンター(静岡)
    • Year and Date
      2011-03-29
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 機能性分子ラベル化技術を用いた蛋白質・細胞の可視化2011

    • Author(s)
      水上進、渡辺修司、吉村彰真、山本拓、秋元悠里、菊地和也
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡県コンベンションアーツセンター(静岡)
    • Year and Date
      2011-03-28
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 変異型β-ラクタマーゼを用いた細胞内蛋白質ラベル化法の開発2011

    • Author(s)
      水上進、渡辺修司、秋元悠里、菊地和也
    • Organizer
      第1回Vivid Workshop
    • Place of Presentation
      アートランドホテル蓼科(茅野)
    • Year and Date
      2011-03-10
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Functional Molecule Labeling and Photocontrolled Compound Release Technologies for Bioimaging2011

    • Author(s)
      Shin Mizukami, Shuji Watanabe, Mariko Hosoda, Kazuya Kikuchi
    • Organizer
      JSPS Sweden-Japan Joint Colloquium
    • Place of Presentation
      Lund University(Lund、Sweden)
    • Year and Date
      2011-01-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Functional Molecule Labeling and Photocontrolled Compound Release Technologies for Bioimaging2011

    • Author(s)
      Shin Mizukami, Shuji Watanabe, Mariko Hosoda, Kazuya Kikuchi
    • Organizer
      JSPS Sweden-Japan Joint Colloquium
    • Place of Presentation
      Lund University (Lund, Sweden)
    • Year and Date
      2011-01-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Photocontrolled compound release using caged antimicrobial peptide2011

    • Author(s)
      Shin Mizukami, Kengo Matsumoto, Kazuya Kikuchi
    • Organizer
      Grordon Research Conference, Bioorganic Chemistry
    • Place of Presentation
      Procter Academy (NH, USA)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Fast Fluorogenic Protein Labeling with Designed FRET Probes and Application to Real-Time Pulse-Chase Analysis2011

    • Author(s)
      Shin Mizukami, Shuji Watanabe, Yuri Akimoto, Kazuya Kikuchi
    • Organizer
      Imaging 2020
    • Place of Presentation
      Jackson Lake Lodge (WY, USA)
    • Related Report
      2011 Annual Research Report
  • [Presentation] UV light-induced Compound Release Using a Caged Antimicrobial Peptide2010

    • Author(s)
      Shin Mizukami, Mariko Hosoda, Kazuya Kikuchi
    • Organizer
      Pacifichem 2010
    • Place of Presentation
      Hawaii Convention Center(Waikiki, HI)
    • Year and Date
      2010-12-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of a pH-responsive MRI probe using a functional polymer2010

    • Author(s)
      Shin Mizukami, Shuji Watanabe, Kazuya Kikuchi
    • Organizer
      2010 World Molecular Imaging Congress
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2010-09-08
    • Related Report
      2011 Final Research Report
  • [Presentation] UV light-induced Compound Release Using a Caged Antimicrobial Peptide2010

    • Author(s)
      Shin Mizukami, Satoshi Okada, Kazuya Kikuchi
    • Organizer
      第20回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      徳島文理大学(徳島)
    • Year and Date
      2010-06-25
    • Related Report
      2011 Final Research Report
  • [Presentation] β-ラクタマーゼを用いた分子イメージング基盤技術の開発2010

    • Author(s)
      水上進、渡辺修司、吉村彰真、松下尚嗣、菊地和也
    • Organizer
      第5回日本分子イメージング学会学術集会
    • Place of Presentation
      ピアザ淡海(大津)
    • Year and Date
      2010-05-22
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 変異β-ラクタマーゼを用いた新規蛋白質ラベル化法の開発2010

    • Author(s)
      水上進、渡辺修司、菊地和也
    • Organizer
      日本ケミカルバイオロジー学会第五回年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(横浜)
    • Year and Date
      2010-05-18
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 光感受性抗菌ペプチドを用いた薬物放出システムの開発2010

    • Author(s)
      水上進・細田真梨子・菊地和也
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山コンベンションセンター(岡山)
    • Year and Date
      2010-03-28
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 多機能性タンパク質ラベル化技術の開発とイメージングへの応用2010

    • Author(s)
      水上進・渡辺修司・山本拓・吉村彰真・菊地和也
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山コンベンションセンター(岡山)
    • Year and Date
      2010-03-28
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Development of a pH-responsive MRI probe using a functional polymer2010

    • Author(s)
      Shin Mizukami, Satoshi Okada, Kazuya Kikuchi
    • Organizer
      第20回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      徳島文理大学(徳島)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Protein-labeling technology based on a mutant β-lactamase and its functional substrates2010

    • Author(s)
      Shin Mizukami, Shuji Watanabe, Kazuya Kikuchi
    • Organizer
      2010 World Molecular Imaging Congress
    • Place of Presentation
      京都国際会館(京都)
    • Related Report
      2010 Annual Research Report
  • [Presentation] UV light-induced Compound Release Using a Caged Antimicrobial Peptide2010

    • Author(s)
      Shin Mizukami, Mariko Hosoda, Kazuya Kikuchi
    • Organizer
      Pacifichem 2010
    • Place of Presentation
      Hawaii Convention Center (Waikiki, HI)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 希土類金属錯体を用いたin vivoイメージング用プローブの開発2009

    • Author(s)
      水上進
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      アスティ徳島(徳島)
    • Year and Date
      2009-10-30
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Development of MRI Probes for Detecting Biological Signals2009

    • Author(s)
      Shin Mizukami, Hisashi Matsushita, Kazuya Kikuchi
    • Organizer
      Gordon Research Conference-Bioorganic Chemistry
    • Place of Presentation
      Procter Academy, Andover, NH, USA.
    • Year and Date
      2009-06-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 化学原理に基づくバイオイメージングツール開発2009

    • Author(s)
      水上進
    • Organizer
      第2回稲盛フロンティア研究講演会
    • Place of Presentation
      九州大学伊都キャンパス(福岡)
    • Year and Date
      2009-05-19
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 光応答性薬物放出システムの開発2009

    • Author(s)
      水上進・佐竹孝文・菊地和也
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      神戸市産業振興センター(神戸)
    • Year and Date
      2009-05-18
    • Related Report
      2011 Final Research Report
  • [Presentation] レポーター酵素活性を検出する^19F MRIプローブ2009

    • Author(s)
      水上進・松下尚嗣・滝川利佳・菊地和也
    • Organizer
      日本ケミカルバイオロジー学会第4回年会
    • Place of Presentation
      学術総合センター(東京都千代田区)
    • Year and Date
      2009-05-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 希土類金属錯体を用いたin vivoイメージング用プローブの開発2009

    • Author(s)
      Shin Mizukami
    • Organizer
      Gordon Research Conference - Bioorganic Chemistry
    • Place of Presentation
      Procter Academy, Andover, NH, USA
    • Related Report
      2009 Annual Research Report
  • [Presentation] 光応答性薬物放出システムの開発2009

    • Author(s)
      水上進・佐竹孝文・菊地和也
    • Organizer
      日本ケミカルバイオロジー学会第4回年会
    • Place of Presentation
      神戸市産業振興センター(神戸)
    • Related Report
      2009 Annual Research Report
  • [Presentation] レポーター酵素活性を検出する^<19>F MRIプローブ2009

    • Author(s)
      水上進・松下尚嗣・滝川利佳・菊地和也
    • Organizer
      日本分子イメージング学会第4回学会総会・学術集会
    • Place of Presentation
      学術総合センター(東京都千代田区)
    • Related Report
      2009 Annual Research Report
  • [Book] 「機能性分子設計に基づく蛋白質の蛍光ラベル化」蛍光イメージング/ MRIプローブの開発2011

    • Author(s)
      水上進・菊地和也
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Final Research Report
  • [Book] 「酵素活性を検出する19F MRIプローブの開発」蛍光イメージング/ MRIプローブの開発2011

    • Author(s)
      水上進・菊地和也
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Final Research Report
  • [Book] 蛍光イメージング/MRIプローブの開発第9章機能性分子設計に基づく蛋白質の蛍光ラベル化2011

    • Author(s)
      水上進・菊地和也
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Annual Research Report
  • [Book] 蛍光イメージング/MRIプローブの開発第11章酵素活性を検出する^<19>F MRIプローブの開発2011

    • Author(s)
      水上進・菊地和也
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Annual Research Report
  • [Book] 「金属イオンプローブを用いた生体機能イメージング」化学フロンティア-生命現象を理解する分子ツール2010

    • Author(s)
      水上進・松下尚嗣・菊地和也
    • Publisher
      化学同人
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www-molpro.mls.eng.osaka-u.ac.jp/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www-molpro.mls.eng.osaka-u.ac.jp/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www-molpro.mls.eng.osaka-u.ac.jp/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www-molpro.mls.eng.oska-u.ac.jp/

    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] タンパク質を蛍光標識する方法2012

    • Inventor(s)
      Kazuya Kikuchi, Shin Mizukami, Shuji Watanabe, Yuri Akimoto
    • Industrial Property Rights Holder
      Osaka University
    • Filing Date
      2012-02-01
    • Related Report
      2011 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 特許2012

    • Inventor(s)
      Kazuya Kikuchi, Shin Mizukami, Shuji Watanabe, Yuri Akimoto
    • Industrial Property Rights Holder
      Osaka University
    • Filing Date
      2012-02-01
    • Related Report
      2011 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] タンパク質を蛍光標識する方法2011

    • Inventor(s)
      水上進、渡辺修司、秋元悠里、菊地和也
    • Industrial Property Rights Holder
      大阪大学
    • Industrial Property Number
      2010-020804
    • Filing Date
      2011-02-02
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] 特許2011

    • Inventor(s)
      水上進、渡辺修司、秋元悠里、菊地和也
    • Industrial Property Rights Holder
      大阪大学
    • Industrial Property Number
      2010-020804
    • Filing Date
      2011-02-02
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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