Control of membrane fouling in MBRs on the basis of proteome analysis and measurements of atomic force

• Project/Area Number: 21686050
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Civil and environmental engineering
• Research Institution: Hokkaido University
• Principal Investigator: KIMURA Katsuki 北海道大学, 大学院・工学研究院, 准教授 (10292054)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2009: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 下水処理 / 膜処理 / 膜分離活性汚泥法 / 膜ファウリング / タンパク質 / MBR / 2D-PAGE / 代謝産物 / 原子間力顕微鏡

Research Abstract

In this study, structure of proteins that were involved in membrane fouling in membrane bioreactors(MBRs) was investigated. Proteins causing membrane fouling were obtained from fouled membranes used in an MBR treating municipal wastewater. Modification of sample pre-treatment enabled application of two-dimensional gel electrophoresis and the protein causing membrane fouling could be separated on the basis of molecular weights and isoelectric points. The separated proteins were subjected to amino acid sequence analysis. It was shown that outer membrane proteins of bacteria belonging to the genus Pseudomonas played an important role in the evolution of membrane fouling in MBRs.

Research Products

• [Journal Article] Characteristics of foulants in air-sparged side-stream tubular membranes used in a municipal wastewater membrane bioreactor (2012)
  • Author(s): Hoque, A., Kimura, K., Miyoshi, T., Yamato, N., Watanabe, Y.
  • Journal Title: Separation and Purification Technology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Performance of Membrane Bio-reactor Equipped with Air-sparged Side-stream Tubular Membrane : Treatment Efficiency and Membrane Fouling (2012)
  • Author(s): Hoque, A., Miyoshi, T., Kimura, K., Watanabe, Y.
  • Journal Title: Separation Science and Technology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Characteristics of proteins involved in membrane fouling in membrane bioreactors(MBRs) treating municipal wastewater : the application of metaproteomic analyses (2011)
  • Author(s): Miyoshi, T., Aizawa, T., Kimura, K., Watanabe, Y.
  • Journal Title: Desalination and Water Treatment Volume: Vol.34(1-3) Pages: 150-155
  • NAID: 120005228167
  • Peer Reviewed
• [Journal Article] Characteristics of proteins involved in membrane fouling in membrane bioreactors (MBRs) treating municipal wastewater : the application of metaproteomic analyses (2011)
  • Author(s): Miyoshi, T., Aizawa, T., Kimura, K., Watanabe, Y.
  • Journal Title: Desalination and Water Treatment Volume: 34(1-3) Issue: 1-3 Pages: 150-155
  • DOI: 10.5004/dwt.2011.2894
  • NAID: 120005228167
  • Peer Reviewed
• [Journal Article] Fouling potentials of polysaccharides in membrane bioreactors (MBAs) assessed by lectin affinity chromatography (2010)
  • Author(s): Taro Miyoshi, et al.
  • Journal Title: Water Science and Technology Volume: 61 Pages: 1787-1792
  • Peer Reviewed
• [Journal Article] Influence of membrane properties on physically reversible and irreversible fouling in membrane bioreactors (2010)
  • Author(s): Tomoo Tsuyuhara, et al.
  • Journal Title: Water Science and Technology Volume: 61 Pages: 2235-2240
  • Peer Reviewed
• [Journal Article] Membrane fouling in nanofiltration/reverse osmosis membranes coupled with a membrane bioreactor used for municipal wastewater treatment (2010)
  • Author(s): Naoko Ogawa, et al.
  • Journal Title: Desalination and Water Treatment Volume: 18 Pages: 292-296
  • Peer Reviewed
• [Journal Article] Seasonal variation in membrane fouling in membrane bioreactors(MBRs)treating municipal wastewater (2009)
  • Author(s): Miyoshi, T., Tsuyuhara, T., Ogyu, R., Kimura, K., Watanabe, Y.
  • Journal Title: Water Research 43 Pages: 5109-5118
  • NAID: 120001909643
  • Peer Reviewed
• [Journal Article] 水温の異なる時期における多糖類のファウリングポテンシャルの変化 (2009)
  • Author(s): 三好太郎、田中一平、木村克輝、渡辺義公
  • Journal Title: 環境工学研究論文集 46 Pages: 645-652
  • Peer Reviewed
• [Presentation] 槽外型MBRに装着したセラミック膜における膜ファウリング原因物質の分析 (2012)
  • Author(s): 安井信人、三好太郎、木村克輝、大和信大、渡辺義公
  • Organizer: 第46回日本水環境学会年会
  • Place of Presentation: 東洋大学(東京都)
  • Year and Date: 2012-03-16
• [Presentation] 担体投入が浸漬型MBRにおける膜ファウリングの発生に及ぼす影響 (2012)
  • Author(s): 栗田宗大、三好太郎、木村克輝、渡辺義公
  • Organizer: 第46回日本水環境学会年会
  • Place of Presentation: 東洋大学(東京都)
  • Year and Date: 2012-03-16
• [Presentation] MBRファウリング多糖のMALDI-TOFMS分析における試料調製条件の検討 (2012)
  • Author(s): 田村尚也、三好太郎、木村克輝、渡辺義公
  • Organizer: 第46回日本水環境学会年会
  • Place of Presentation: 東洋大学(東京都)
  • Year and Date: 2012-03-16
• [Presentation] 槽外型セラミック膜に装着したセラミック膜における膜ファウリング (2011)
  • Author(s): 安井信人、三好太郎、木村克輝、渡辺義公
  • Organizer: 第48回環境工学研究フォーラム
  • Place of Presentation: 大同大学(名古屋市)
  • Year and Date: 2011-11-26
• [Presentation] 担体投入に伴うMBRの運転効率の改善 (2011)
  • Author(s): 栗田宗大、三好太郎、木村克輝、渡辺義公
  • Organizer: 第48回環境工学研究フォーラム
  • Place of Presentation: 大同大学(名古屋市)
  • Year and Date: 2011-11-26
• [Presentation] 膜分離活性汚泥法において膜ファウリングを引き起こしているタンパク質のメタプロテオーム解析 (2011)
  • Author(s): 三好太郎、相沢智康、木村克輝、渡辺義公
  • Organizer: 土木学会第66回年次学術講演会
  • Place of Presentation: 愛媛大学(松山市)
  • Year and Date: 2011-09-07
• [Presentation] Characteristics of proteins involved in membrane fouling in membrane bioreactors (MBRs) treating municipal wastewater : the application of metaproteomic analyses (2010)
  • Author(s): Taro Miyoshi, et al.
  • Organizer: AMS6
  • Place of Presentation: Hilton Sydney、Sydney, Australia
  • Year and Date: 2010-11-24
• [Presentation] 膜分離活性汚泥法において膜ファウリングに関与しているタンパク質の構造解析 (2010)
  • Author(s): 三好太郎, 他
  • Organizer: 第47回環境工学研究フォーラム
  • Place of Presentation: 高知大学、高知
  • Year and Date: 2010-11-13
• [Presentation] Effect of hydraulic condition on membrane fouling in airlift side-stream membrane bioreactor (2010)
  • Author(s): Asiful Hoque, et al.
  • Organizer: MDIW2010
  • Place of Presentation: Rica Nidelven Hotel Trondheim, Norway
  • Year and Date: 2010-06-29
• [Presentation] Metaproteomic analysis of proteins causing physically irreversible fouling in membrane bioreactors (MBRs) (2010)
  • Author(s): Taro Miyoshi, et al.
  • Organizer: MDIW2010
  • Place of Presentation: Rica Nidelven Hotel Trondheim, Norway
  • Year and Date: 2010-06-28
• [Presentation] 膜分離活性汚泥法(MBR)において膜ファウリングに関与しているタンパク質のプロテオーム解析 (2010)
  • Author(s): 三好太郎、木村克輝、相沢智康、渡辺義公
  • Organizer: 第44回日本水環境学会年会
  • Place of Presentation: 福岡大学、福岡
  • Year and Date: 2010-03-15
• [Presentation] Fouling potentials of polysaccharides in MBRs assessed by lectin affinity chromatography (2009)
  • Author(s): Miyoshi, T., Tanaka, T., Tsuyuhara, T., Watanabe, E., Aizawa, T., Kimura, K., Watanabe, Y.
  • Organizer: IWA Membrane Technology Conference & Exhibition 2009
  • Place of Presentation: 北京中国
  • Year and Date: 2009-09-02