Development of antibody screening system in mammalian cells using chimeric receptors
| Project/Area Number |
21686077
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Keywords | 抗体 / 受容体 / 細胞増殖 / ライブラリー選択 / シグナル伝達 |
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| Research Abstract |
In this study, we aimed to develop a novel method to rapidly and easily select antigen-specific antibody from an antibody library based on growth activity of mammalian cells. To achieve this, fusion proteins composed of an antibody library and a growth-inducing receptor were expressed on mammalian cells, and the resultant cells were cultured in the medium containing a dimeric target antigen, followed by analysis of antigen binding and specificity of the selected cells. As a result, antigen-binding antibody fragments were successfully obtained based on the cell growth.
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Report
(4 results)
Research Products
(57 results)
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[Journal Article] Growth promotion ofgenetically modified hematopoieticprogenitors using an antibody/c-Mpl chimera2011
Author(s)
Kawahara, M., Chen, J., Sogo, T., Teng, J., Otsu, M., Onodera, M., Nakauchi, H., Ueda, H. and Nagamune, T.
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Journal Title
Cytokine
Volume: 55
Issue: 3
Pages: 402-408
DOI
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Peer Reviewed
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[Journal Article] T cell growth control usinghapten-specific antibody/interleukin-2receptor chimera2009
Author(s)
Sogo, T., Kawahara, M., Ueda, H., Otsu, M., Onodera, M., Nakauchi, H. and Nagamune, T.
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Journal Title
Cytokine
Volume: 46
Issue: 1
Pages: 127-136
DOI
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[Book] Animal Cell Technology : Basic & Applied Aspects2010
Author(s)
Kawahara, M., Inoue, T., Ren, X., Sogo, T., Yamada, H., Katoh, M., Ueda, H., Oshimura, M. and Nagamune, T.
Publisher
Growth control ofmammalian cells via a human artificialchromosome harboring a chimeric receptor
Related Report
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[Book] Animal Cell Technology : Basic & Applied Aspects2009
Author(s)
Liu, W., Kawahara, M., Ueda, H., and Nagamune, T.
Publisher
Construction of afluorescein-responsive chimeric receptorwith strict ligand dependency and analysis ofthe role of erythropoietin receptor domainsin signal transduction
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