Identificationand the analysis of the genes involvedin photoreceptor morphogenesis and maintenance in Drosophila

• Project/Area Number: 21687005
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: Hiroshima University (2011); Nagoya University (2009-2010)
• Principal Investigator: SATOH Akiko 広島大学, 大学院・総合科学研究科, 准教授 (30529037)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000); Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2010: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2009: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: ショウジョウバエ / 視細胞 / ロドプシン・アレスチン / GPI結合タンパク質 / トランスゴルジネットワーク / 網膜 / 形態形成 / 小胞輸送 / 明暗順応 / ロドプシン / 極性輸送 / 色素顆粒 / スクリーニング

Research Abstract

We screened to identify new genes involved in Rhodopsin transport andfound two that the deficiency of glycosylphosphatidylinositol (GPI) synthesis/attachment processes causes the loss of the apical transport of Rhodopsinfrom the TGN (Development, 2013). In the process of studying Arrstin mediated signaling, we found that Arr2 translocation in Drosophilaphotoreceptors is driven by diffusion, but profoundly accelerated by phototransduction and Ca2+influx (Neuron 2010, J. Neursci. 2012)

Research Products

• [Journal Article] GPI biosynthesis is essential for Rhodopsin sorting at the trans-Golgi network in Drosophila photoreceptors (2013)
  • Author(s): Satoh, T., Inagaki, T., Liu, J., Watanabe, R. and Satoh, A. K
  • Journal Title: Development Volume: 140 Pages: 385-94
  • Peer Reviewed
• [Journal Article] GPI biosynthesis is essential for Rhodopsin sorting at the trans-Golgi network in Drosophila photoreceptors. (2013)
  • Author(s): Satoh, T., Inagaki, T., Watanabe, R. and Satoh, A. K.# (#: corresponding author)
  • Journal Title: Development Volume: 140 Issue: 2 Pages: 385-94
  • DOI: 10.1242/dev.083683
  • Peer Reviewed
• [Journal Article] Regulation of arrestin translocation by Ca2+and Myosin III in Drosophila photoreceptors (2012)
  • Author(s): Hardie, R. C., Satoh, A. K. and Liu, C-H
  • Journal Title: J. Neurosci Volume: 32 Pages: 9205-16
  • Peer Reviewed
• [Journal Article] ショウジョウバエ視細胞における色素顆粒運動の分子機構 (2011)
  • Author(s): 佐藤明子
  • Journal Title: 生化学 Volume: 83 Pages: 1032-5
• [Journal Article] Arrestin translocation is stoichiometric to rhodopsin isomerization and accelerated by phototransduction in Drosophila photoreceptors. (2010)
  • Author(s): Satoh, A. K.#, Xia, H.#, Yan, L., Huang J., Hardie R. C. and Ready, D. F
  • Journal Title: Neuron Volume: 67 Pages: 997-1008
  • Peer Reviewed
• [Journal Article] ショウジョウバエ視細胞において、アレスチンのトランスロケーションは光情報変換系により促進され、その絶対量はロドプシンの異性化量に対して化学量論的である (2010)
  • Author(s): 佐藤明子
  • Journal Title: ライフサイエンス新着レビュー
• [Journal Article] ショウジョウバエロドプシンの輸送機構 (2010)
  • Author(s): 佐藤明子
  • Journal Title: 新学術領域「細胞内ロジスティクス」Newsletter Volume: 3 Pages: 6-10
• [Presentation] Drosophila dPobはロドプシンの合成中間体の安定化に必要である (2012)
  • Author(s): 佐藤卓至, 大場綾, Ziguang Liu, 稲垣壮, 佐藤明子
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-16
• [Presentation] ショウジョウバエロドプシンの選別輸送にはGPI生合成が必要である (2012)
  • Author(s): 佐藤卓至, 稲垣壮, Ziguang Liu, 渡辺玲香, 佐藤明子
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] dPobはロドプシンのフォールディングに必要である (2012)
  • Author(s): 佐藤卓至, 大場綾, Ziguang Liu, 稲垣壮, 佐藤明子
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] ショウジョウバエ視細胞における色素顆粒運動に関わる因子の変異体探索 (2012)
  • Author(s): 山本詩織, 佐藤卓至, 杉山伸, 尾崎浩一, 佐藤明子
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] biosynthesis is essential for the sorting of Rh1 at the trans Golgi network in Drosophila photoreceptors. (2012)
  • Author(s): Satoh, T., Inagaki, T., Liu, J., Watanabe, R. and Satoh, A. K
  • Organizer: Gordon Research Conference on Visual system development
  • Place of Presentation: New London at UAS
  • Year and Date: 2012-08-21
• [Presentation] The mechanism of Rhodopsin transport to the photoreceptive membrane, rhabdomeres in Drosophila photoreceptors. (2011)
  • Author(s): 佐藤明子
  • Organizer: 第82回日本生化学会大会. シンポジウム"オルガネラ研究新地平
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] GPI biosynthesis is essential for the sorting of Rh1 at the trans Golgi network in Drosophila photoreceptors. (2011)
  • Author(s): Satoh, T., Inagaki, T., Watanabe, R. and Satoh, A. K
  • Organizer: FASEB Summer Research Conference on Protein lipidation, signaling, and membrane domains
  • Place of Presentation: axton River at UAS
  • Year and Date: 2011-07-27
• [Presentation] in vivo cell biology : a model system of Drosophila eye, two tales of Rhodopsin transport and pigment granules migration. (2010)
  • Author(s): 佐藤明子
  • Organizer: International Symposium "Beyond the frontier of Genetics"
  • Place of Presentation: 国立遺伝学研究所
  • Year and Date: 2010-10-14
• [Presentation] ショウジョウバエ視細胞の構造形成と機能維持機構の解明 (2010)
  • Author(s): 佐藤明子
  • Organizer: 動物学会 奨励賞受賞者講演
  • Place of Presentation: 東京大学
  • Year and Date: 2010-09-24
• [Presentation] アレスチントランスロケーションは光情報伝達シグナルにより加速される (2010)
  • Author(s): 佐藤明子
  • Organizer: 第82回日本動物学会大会.
  • Place of Presentation: 東京
  • Year and Date: 2010-09-23
• [Presentation] アレスチンの移行は光情報変換系により加速される (2010)
  • Author(s): 佐藤明子
  • Organizer: 動物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-09-23
• [Presentation] ロドプシン輸送を阻害する変異体のスクリーニング (2009)
  • Author(s): 佐藤明子
  • Organizer: 第81回日本動物学会大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-18
• [Presentation] GPI biosynthesis is essential for the sorting of Rh1 at the trans Golgi network in Drosophila photoreceptors.
  • Author(s): Satoh, T., Inagaki, T., Watanabe, R. and (Satoh, A. K.)
  • Organizer: FASEB Summer Research Conference on Protein lipidation, signaling, and membrane domains
  • Place of Presentation: Saxton River at UAS
• [Presentation] The mechanism of Rhodopsin transport to the photoreceptive membrane, rhabdomeres in Drosophila photoreceptors.
  • Author(s): (佐藤明子)
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 京都
  • Invited
• [Presentation] GPI biosynthesis is essential for the sorting of Rh1 at the trans Golgi network in Drosophila photoreceptors
  • Author(s): Satoh, T., Inagaki, T., Liu, J., Watanabe, R. and (Satoh, A. K. )
  • Organizer: Gordon Research Conference on Visual system development
  • Place of Presentation: New London at UAS
• [Presentation] ショウジョウバエロドプシンの選別輸送にはGPI生合成が必要である
  • Author(s): 佐藤卓至, 稲垣壮, Ziguang Liu, 渡辺玲香, (佐藤明子)
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪
• [Presentation] dPobはロドプシンのフォールディングに必要である
  • Author(s): 佐藤卓至, 大場綾, Ziguang Liu, 稲垣壮,(佐藤明子)
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪
• [Presentation] ショウジョウバエ視細胞における色素顆粒運動に関わる因子の変異体探索
  • Author(s): 山本詩織, 佐藤卓至, 杉山伸, 尾崎浩一, 佐藤明子.
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪