Archaeal hunting : explore new metabolic functions of uncultured Archaea by using new culturing techniques
Project/Area Number |
21687006
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Biodiversity/Systematics
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Research Institution | Japan Agency for Marine-Earth Science and Technology |
Principal Investigator |
IMACHI Hiroyuki 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (20361933)
|
Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2011: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2010: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2009: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
|
Keywords | アーキア / 分離培養 / 16S rRNA遺伝子 / メタン / 嫌気的メタン酸化 / 古細菌 / 分離 |
Research Abstract |
Cultured enrichments and isolates are important in developing our understanding of microbial physiology, genetics, and ecology. However, many microorganisms, particularly members of the domain Archaea, remain uncultured. In this study, I attempted to cultivate and isolate uncultured Archaea from various environmental samples using new cultivation techniques I developed. As a result, I successfully cultivated and isolated methanogenic and methanotrophic Archaea. These results strongly suggest that the culturing methods I developed are effective ways to cultivate fastidious but ecologically important archaeal species that would otherwise escape conventional isolation strategies. Also, these isolates obtained in this study would promote to understand carbon cycle on earth and to develop new environmental technologies.
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Report
(4 results)
Research Products
(56 results)
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[Journal Article] Methanolinea mesophila, sp. nov., a hydrogenotrophic methanogen isolated from a rice field in Taiwan, and proposal of the new archaeal family Methanoregulaceae fam. nov2012
Author(s)
Sakai, S., M. Ehara, I-C. Tseng, T. Yamaguchi, S. L. Brauer, H. Cadillo-Quiroz, S. H. Zinder and H. Imachi
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Journal Title
Int. J. Syst
Volume: 62
Pages: 1389-1395
Related Report
Peer Reviewed
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[Journal Article] Cultivation of methanogenic community from subseafloor sediments using a continuous-flow bioreactor2011
Author(s)
Imachi, H., K. Aoi, E. Tasumi, Y. Saito, Y. Yamanaka, Y. Saito, T. Yamaguchi, H. Tomaru, R. Takeuchi, Y. Morono, F. Inagaki and K. Takai
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Journal Title
ISME J.
Volume: 5
Pages: 1913-1925
Related Report
Peer Reviewed
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