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Histone demethylase Jmjd1a as a regulator of glycolipid metabolism

Research Project

Project/Area Number 21687013
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionThe University of Tokyo

Principal Investigator

INAGAKI Takeshi  東京大学, 先端科学技術研究センター, 特任准教授 (10507825)

Research Collaborator SAKAI Juro  東京大学, 先端科学技術研究センター, 教授 (80323020)
SHINKAI Yoichi  理化学研究所, 主任研究員 (20211972)
TACHIBANA Makoto  京都大学, ウィルス研究所, 准教授 (80303915)
ABURATANI Hiroyuki  東京大学, 先端科学技術研究センター, 教授 (10202657)
KODAMA Tatsuhiko  東京大学, 先端科学技術研究センター, 教授 (90170266)
KAWAMURA Takeshi  東京大学, 先端科学技術研究センター, 助教 (70306835)
OKAMURA Hitoshi  京都大学, 薬学研究科, 教授 (60158813)
DOI Masao  京都大学, 薬学研究科, 准教授 (20432578)
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywordsエピゲノム / ヒストン脱メチル化酵素 / Jmjd1a / 糖脂質代謝調節 / ヒストン / Jmjdla / 肥満 / ChIPシークエンス / RNAシークエンス / ヒストンメチル化 / エビゲノム / H3K9 / Jhdm2a / 代謝 / 糖脂質代謝 / ChIP
Research Abstract

Jmjd1a (also known as Jhdm2a and KDM3a) catalyzes removal of H3K9 mono- and dimethylation. We established JMJD1a deficient mice (JMJD1A -/-) and discovered that the mice develop adult onset obesity, hypertriglyceridemia, hypercholesterolemia, and insulin insensitivity, that are hallmarks of metabolic syndrome. To investigate the molecular mechanism of Jmjd1a working machinery, we sought to elucidate Jmjd1a target genes and working complex using techniques of chromatin immonoprecipitation and mass spectrometry. We raised monoclonal antibodies against mouse and human Jmjd1a protein prepared by baculovirus expression system. ChIP sequence using Jmjd1a antibody as well as methylated histone antibodies showed a specific binding region which codes a retrotransposon. Whole cell extracted protein from HeLa cells was immunoprecipitated using the established Jmjd1a antibodies and the precipitated protein complex was analyzed using shotgun mass spectrometry. Interestingly, a series of splicing factors and sin3a complex forming proteins appeared in the list of shotgun proteomics. It is also elucidated that there is a functional phosphorylated site in Jmjd1a. Our result indicates that Jmjd1a regulates gene transcription through changing the chromatin formation.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (37 results)

All 2012 2011 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (20 results) (of which Invited: 1 results) Remarks (12 results)

  • [Journal Article] Dynamic change of chromatin conformation in response to hypoxia enhances the expression of GLUT3 (SLC2A3) by cooperative interaction of hypoxia-inducible factor 1 and KDM3A.2012

    • Author(s)
      Mimura I
    • Journal Title

      Mol Cell Biol.

      Volume: Aug;32(15) Issue: 15 Pages: 3018-3032

    • DOI

      10.1128/mcb.06643-11

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of histone methylation and demethylation in adipogenesis and obesity2010

    • Author(s)
      Okamura M., Inagaki T., Tanaka T., Sakai J
    • Journal Title

      Organogenesis

      Volume: 6(1) Pages: 24-32

    • URL

      http://dx.doi.org/10.4161/org.6.1.11121

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of histone methylation and demethylation in adipogenesis and obesity.2010

    • Author(s)
      Okamura M., Inagaki T., 他
    • Journal Title

      Organogenesis

      Volume: 6(1) Pages: 24-32

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Obesity and Metabolic Syndrome in Histone Demethylase JHDM2a Deficient Mice2009

    • Author(s)
      Inagaki T., Tachibana M., Magoori K., Kudo H., Tanaka T., Okamura M., Naito M., Kodama T., Shinkai Y., Sakai J
    • Journal Title

      Genes to Cells

      Volume: 14(8) Issue: 8 Pages: 991-1001

    • DOI

      10.1111/j.1365-2443.2009.01326.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Obesity and Metabolic Syndrome in Histone Demethylase JHDM2a Deficient Mice2009

    • Author(s)
      Inagaki T, et al
    • Journal Title

      Genes to Cells 14

      Pages: 991-1001

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 核内受容体によるFGF制御とエピゲノムを介した糖脂質代謝調節2012

    • Author(s)
      稲垣毅
    • Organizer
      京都成人血管病シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2012-12-05
    • Related Report
      2012 Final Research Report
  • [Presentation] 転写因子とエピゲノムを介したエネルギー代謝の制御機構2011

    • Author(s)
      稲垣毅
    • Organizer
      平成23年度第1回応用動物科学セミナー
    • Place of Presentation
      東京大学農学部(東京都)(招待講演)
    • Year and Date
      2011-07-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒストン脱メチル化酵素Jmjd1aによる代謝調節2011

    • Author(s)
      稲垣毅、立花誠、眞貝洋一、酒井寿郎
    • Organizer
      第84回日本内分泌学会学術総会(シンポジウム)
    • Place of Presentation
      神戸
    • Year and Date
      2011-04-23
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒストン脱メチル化酵素Jmjd1aによる代謝調節2011

    • Author(s)
      稲垣毅、立花誠、眞貝洋一、酒井寿郎
    • Organizer
      第84回日本内分泌学会学術総会
    • Place of Presentation
      神戸国際会議場(兵庫県)(シンポジウム)(招待講演)
    • Year and Date
      2011-04-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Transcriptional and Epigenetic Regulations of Metabolism2011

    • Author(s)
      稲垣毅
    • Organizer
      日本ベーリンガーインゲルハイム 神戸医薬研究所セミナー
    • Place of Presentation
      日本ベーリンガーインゲルハイム 神戸医薬研究所(兵庫県)(招待講演)
    • Year and Date
      2011-04-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] TranscriptionalandEpigeneticRegulationsofMetabolism2011

    • Author(s)
      稲垣毅
    • Organizer
      熊本大学発生医学研究所グローバルCOEリエゾンラボ研究会
    • Place of Presentation
      熊本
    • Year and Date
      2011-03-09
    • Related Report
      2012 Final Research Report
  • [Presentation] Transcriptional and Epigenetic Regulations of Metabolism2011

    • Author(s)
      稲垣毅
    • Organizer
      熊本大学発生医学研究所グローバルCOEリエゾンラボ研究会
    • Place of Presentation
      熊本大学
    • Year and Date
      2011-03-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Metabolic regulation by the histone demethylase Jmjdla2011

    • Author(s)
      稲垣毅
    • Organizer
      第9回システム生物医学研究会
    • Place of Presentation
      箱根湯本富士屋ホテル
    • Year and Date
      2011-02-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒストン脱メチル化酵素Jmjd1aによる糖脂質代謝調節2010

    • Author(s)
      稲垣毅、立花誠、眞貝洋一、酒井寿郎
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会(BMB2010)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒストン脱メチル化酵素Jmjdlaによる糖脂質代謝調節2010

    • Author(s)
      稲垣毅, 他
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会(BMB2010)他
    • Place of Presentation
      神戸ポートピアホテル
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒストンリジンメチル化修飾による代謝制御2010

    • Author(s)
      稲垣毅
    • Organizer
      京都大学大学院薬学研究科医薬創成情報科学講座システムバイオロジー分野第17回システムバイオロジーセミナー
    • Place of Presentation
      京都大学大学院
    • Year and Date
      2010-12-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Glycolipid Metabolism Regulation by Fibroblast Growth Factors2010

    • Author(s)
      稲垣毅
    • Organizer
      第53回日本糖尿病学会年次学術集会(シンポジウム)
    • Place of Presentation
      岡山
    • Year and Date
      2010-05-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Glycolipid Metabolism Regulation by Fibroblast Growth Factors2010

    • Author(s)
      稲垣毅
    • Organizer
      第53回日本糖尿病学会年次学術集会
    • Place of Presentation
      岡山全日空ホテル(シンポジウム)
    • Year and Date
      2010-05-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 「ヒストン脱メチル化酵素によ る代謝調節」東京2010

    • Author(s)
      稲垣毅
    • Organizer
      Keystone symposia, adipose tissue biology
    • Place of Presentation
      Keystone, Colorado, USA
    • Year and Date
      2010-01-25
    • Related Report
      2012 Final Research Report
  • [Presentation] Histone demethylase JMJDla regulates fat storage2010

    • Author(s)
      Takeshi Inagaki, et al
    • Organizer
      Keystone Symposia
    • Place of Presentation
      Keystone, CO, USA
    • Year and Date
      2010-01-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Obesity and Metabolic Syndrome in Histone Demethylase JHDM2a Deficient Mice2009

    • Author(s)
      稲垣毅
    • Organizer
      第6回インスリン抵抗性とメタボリックシンドローム研究会
    • Place of Presentation
      東京
    • Year and Date
      2009-08-11
    • Related Report
      2012 Final Research Report
  • [Presentation] 「ヒストン脱メチル化酵素による代謝調節」東京2009

    • Author(s)
      稲垣毅
    • Organizer
      Brainstorming Medical Conference
    • Place of Presentation
      東京
    • Year and Date
      2009-04-18
    • Related Report
      2012 Final Research Report
  • [Presentation] The role of JmjC domain-containing protein Fbxl10/Jhdm1b in adipocyte differentiation and obesity

    • Author(s)
      稲垣毅
    • Organizer
      第9回インスリン抵抗性とメタボリックシンドローム研究会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 核内受容体によるFGF制御とエピゲノムを介した糖脂質代謝調節

    • Author(s)
      稲垣毅
    • Organizer
      京都成人血管病シンポジウム
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] JmjC-domain-containing histone demethylase 1b (Jhdm1b)による脂肪細胞分化制御

    • Author(s)
      稲垣毅
    • Organizer
      第35回日本分子生物学会年会 (ワークショップ)
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.lsbm.org/staff/inagaki.html

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.mm.rcast.u-tokyo.ac.jp

    • Related Report
      2012 Final Research Report
  • [Remarks] 東京大学先端科学技術研究センター代謝医学分野稲垣毅

    • URL

      http://www.mm.rcast.u-tokyo.ac.jp/member/inagaki.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 東京大学先端科学技術研究センター稲垣毅

    • URL

      http://www.rcast.u-tokyo.ac.jp/ja/people/staff-inagaki_takeshi.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 東京大学先端科学技術研究センターシステム生物医学ラボラトリー稲垣毅

    • URL

      http://www.lsbm.org/staff/inagaki.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.lsbm.org/staff/inagaki.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.rcast.u-tokyo.ac.jp/ja/people/staff-inagaki_takeshi.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.mm.rcast.u-tokyo.ac.jp/member/inagaki.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.rcast.u-tokyo.ac.jp/ja/people/staff-inagaki_takeshi.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.mm.rcast.u-tokyo.ac.jp/inagaki.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.lsbm.org/staff/inagaki.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.tsbmi.m.u-tokyo.ac.jp/taisei/t_inagaki.html

    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2019-07-29  

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