Elucidation of functional roles of membrane lipids

• Project/Area Number: 21687016
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Kyoto University
• Principal Investigator: IKENOUCHI Junichi 京都大学, 大学院・工学研究科, 准教授 (10500051)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: 脂質 / 生体膜 / 上皮細胞 / スフィンゴミエリン / シリカ / 質量分析 / ライセニン / アピカル膜 / 間葉細胞 / 細胞膜 / 細胞接着 / F9細胞 / 脂質多様性 / 細胞極性

Research Abstract

The lipid of cell surface membrane accounts for only 1% of total lipids. In this research project, I aimed to determine lipid species enriched at the cell surface membrane and clarify the functional roles of lipids consisting of cell surface membrane. For this purpose, I established a new method to analyze lipid composition of cell surface membrane.

Research Products

• [Journal Article] Upregulated function of mitochondria-associated ER membranes in Alzheimer disease (2012)
  • Author(s): Area-Gomez E, Lara Castillo M, Tambini M, Guardia-Laguarta C, Ad J.C. de Groof Madra M, Ikenouchi J, Umeda M, Bird T, Sturley S, and Schon E
  • Journal Title: EMBO J Volume: 31(21) Issue: 21 Pages: 4106-23
  • DOI: 10.1038/emboj.2012.202
  • Peer Reviewed
• [Journal Article] Lipid polarity is maintained in absence of tight junctions. (2012)
  • Author(s): Ikenouchi J, Suzuki M, Umeda K, Ikeda K, Taguchi R, Kobayashi T, Sato SB, Kobayashi T, Stolz DB, Umeda M
  • Journal Title: J Biol Chem Volume: 287(12) Issue: 12 Pages: 9525-33
  • DOI: 10.1074/jbc.m111.327064
  • Peer Reviewed
• [Journal Article] LSR defines cell corners for tricellular tight junction formation in epithelial cells (2011)
  • Author(s): Masuda S, Oda Y, Sasaki H, Ikenouchi J, Higashi T, Akashi M, Nishi E, Furuse M
  • Journal Title: J Cell Sci Volume: 124(Pt 4) Issue: 4 Pages: 548-55
  • DOI: 10.1242/jcs.072058
  • Peer Reviewed
• [Journal Article] Phosphorylation state regulates the localization of Scribble at adherens junctions and its association with E-cadherin-catenin complexes (2011)
  • Author(s): Yoshihara K, Ikenouchi J, Izumi Y, Akashi M, Tsukita S, Furuse M
  • Journal Title: Exp Cell Res Volume: 317(4) Issue: 4 Pages: 413-22
  • DOI: 10.1016/j.yexcr.2010.12.004
  • Peer Reviewed
• [Journal Article] LSR defines cell corners for tricellular tight junction formation in epithelial cells (2011)
  • Author(s): Masuda S, Oda Y, Sasaki H, Ikenouchi J, Higashi T, Akashi M, Nishi E, Furuse M
  • Journal Title: Journal of Cell Science Volume: 124 Pages: 548-555
  • Peer Reviewed
• [Journal Article] Phosphorylation state regulates the localization of Scribble at adherens junctions and its association with E-cadherin-catenin complexes (2011)
  • Author(s): Yoshihara K, Ikenouchi J, Izumi Y, Akashi M, Tsukita S, Furuse M
  • Journal Title: Experimental Cell Research Volume: 317 Pages: 413-422
  • Peer Reviewed
• [Journal Article] FRMD4A regulates epithelial polarity by connecting Arf6 activation with the PAR complex (2010)
  • Author(s): Ikenouchi J, Umeda M
  • Journal Title: Proc Natl Acad Sci USA Volume: 107(2) Issue: 2 Pages: 748-53
  • DOI: 10.1073/pnas.0908423107
  • Peer Reviewed
• [Journal Article] FRMD4A regulates epithelial polarity by connecting Arf6 activation with the PAR complex. (2010)
  • Author(s): Junichi Ikenouchi, Masato Umeda
  • Journal Title: Proc Natl Acad Sci USA. 107 Pages: 748-753
  • Peer Reviewed
• [Presentation] Supramolecular complexes at tight junctions (2012)
  • Author(s): Ikenouchi J
  • Organizer: In: KyotoUniversity GCOE symposium "Biomembrane and Channels"
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2012-12-10
• [Presentation] 細胞接着装置における超分子複合体の解析 (2012)
  • Author(s): 池ノ内順一
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨(招待講演)
  • Year and Date: 2012-03-27
• [Presentation] 細胞接着装置における超分子複合体の解析 (2012)
  • Author(s): 池ノ内順一
  • Organizer: 第117回 日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨
  • Year and Date: 2012-03-26
• [Presentation] タイトジャンクションにおける超分子複合体の解析 (2011)
  • Author(s): 池ノ内順一
  • Organizer: 第84回 日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] Re-examination of the role of tight junctions as a lipid diffusion barrier (2011)
  • Author(s): 池ノ内順一、梅田一彰、梅田真郷
  • Organizer: 第53回日本脂質生化学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-12
• [Presentation] Re-examination of the role of tight junctions as a lipid diffusion barrier (2011)
  • Author(s): Junichi Ikenouchi, Kazuaki Umeda, Masato Umeda
  • Organizer: 第53回日本脂質生化学会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-05-12
• [Presentation] 上皮細胞の細胞膜ドメインを規定する脂質の探索 (2009)
  • Author(s): 池ノ内順一、梅田真郷
  • Organizer: 第61回日本細胞生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-03
• [Presentation] 上皮細胞の細胞膜ドメインを規定する脂質の探索(若手優秀発表賞受賞講演) (2009)
  • Author(s): 池ノ内順一
  • Organizer: 第61回日本細胞生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-03
• [Book] 上皮細胞の細胞膜における膜ドメイン.実験医学 (2010)
  • Author(s): 池ノ内順一、梅田真郷
  • Publisher: 羊土社
• [Book] 膜ドメインの姿と生体脂質のダイナミクス. 実験医学 (2010)
  • Author(s): 池ノ内順一、藤本豊士
  • Publisher: 羊土社
• [Book] 生体膜における脂質分子の局在および動態のイメージング. 浜地格/二木史朗編. 生命現象を理解する分子ツール (2010)
  • Author(s): 池ノ内順一、梅田真郷
  • Publisher: 化学同人
• [Book] 実験医学 上皮細胞の細胞膜における膜ドメイン機構 (2010)
  • Author(s): 池ノ内順一、梅田真郷
  • Publisher: 羊土社