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Elucidation of membrane fusion machinery in fertilization

Research Project

Project/Area Number 21687018
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionOsaka University

Principal Investigator

INOUE Naokazu  大阪大学, 微生物病研究所, 助教 (50379096)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2010: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords受精 / 膜融合 / 精子 / 卵子
Research Abstract

Despite the biological importance of sperm-egg fusion in fertilization, the molecular mechanisms underlying this step remain largely unknown. We focused on elucidating the molecular mechanisms of sperm-egg fusion by using genetically manipulated mice. In this application, we reached to obtain two aspects about mammalian fertilization as follows. We identified an IZUMO1-interacting protein in spermatozoon. We produced an Ace3 disrupted mouse line, and found the localization of IZUMO1 spread in a little wider area on spermatozoa, but the elimination of ACE3 did not result in a loss of sperm fertilizing ability. Utilizing Izumo1-deficient spermatozoa, we found that the spermatozoa had the ability to pass through the ZP at least twice. Apparently, some spermatozoa that had undergone the acrosome reaction long before contact with the ZP remained capable of crossing the ZP and fertilizing eggs. The concept that acrosome-reacted spermatozoa are unable to bind to the ZP and have lost their fertilizing capacity must be reconsidered.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (28 results)

All 2011 2010 2009 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (10 results) Remarks (5 results)

  • [Journal Article] Acrosome-reacted mouse spermatozoa recovered from the perivitelline space can fertilize other eggs2011

    • Author(s)
      Inoue N, Satouh Y, Ikawa M, Okabe M, Yanagimachi R
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 108 Issue: 50 Pages: 20008-20011

    • DOI

      10.1073/pnas.1116965108

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calsperin Is a Testis-specific Chaperone Required for Sperm Fertility.2011

    • Author(s)
      Ikawa M*#, Tokuhiro K*, Yamaguchi R, Benham AM, Tamura T, Wada I, Satouh Y, Inoue N, Okabe M
    • Journal Title

      J Biol Chem

      Volume: 286 Issue: 7 Pages: 5639-5646

    • DOI

      10.1074/jbc.m110.140152

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The mechanism of sperm-egg interaction and the involvement of IZUMO1 in fusion2011

    • Author(s)
      Inoue N., et al
    • Journal Title

      Asian J Androl

      Volume: 13 Issue: 1 Pages: 81-87

    • DOI

      10.1038/aja.2010.70

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calsperin is a testis-specific chaperone required for sperm fertility.2011

    • Author(s)
      Ikawa, M., Tokuhiro, K, Yamaguchi, R., Benham, A, M., Tamura, T., Wada, I., Satouh, Y., Inoue, N., Okabe, M.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 288(7) Pages: 5639-46

    • NAID

      120006957386

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The mechanism of sperm-egg interaction and the involvement of IZUMO1 in fusion.2011

    • Author(s)
      Inoue, N., Ikawa, M., Okabe, M.
    • Journal Title

      Asian Journal of Andrology

      Volume: 13(1) Pages: 81-7

    • NAID

      120006957388

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection2010

    • Author(s)
      Oshiumi H, Miyashita M, Inoue N, Okabe M, Matsumoto M, Seya T
    • Journal Title

      Cell Host Microbe

      Volume: 8 Issue: 6 Pages: 496-509

    • DOI

      10.1016/j.chom.2010.11.008

    • NAID

      120002725554

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and disruption of sperm-specific angiotensin converting enzyme-3(ACE3) in mouse2010

    • Author(s)
      Inoue N, Kasahara T, Ikawa M, Okabe M
    • Journal Title

      P LoS One

      Volume: 5

    • NAID

      120006957389

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse2010

    • Author(s)
      Fujihara Y, Murakami M, Inoue N, Satouh Y, Kaseda K, Ikawa M, Okabe M
    • Journal Title

      J Cell Sci

      Volume: 123(Pt 9) Issue: 9 Pages: 1531-1536

    • DOI

      10.1242/jcs.067363

    • NAID

      120006957390

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fertilization: a sperm's journey to and interaction with the oocyte.2010

    • Author(s)
      Ikawa M, Inoue N, Benham AM, Okabe M#.
    • Journal Title

      J Clin Invest

      Volume: 120 Issue: 4 Pages: 984-994

    • DOI

      10.1172/jci41585

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection.2010

    • Author(s)
      Oshiumi, H., Miyashita, M., Inoue, N., Okabe, M., Matsumoto, M., Seya, T.
    • Journal Title

      Cell Host Microbe

      Volume: 8(6) Pages: 496-509

    • NAID

      120002725554

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fertilization : sperm's journey to and interaction with the oocyte2010

    • Author(s)
      Ikawa, M., Inoue, N., Benham, A., Okabe M.
    • Journal Title

      Journal of clinical investigation

      Volume: 120(4) Pages: 984-94

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse2010

    • Author(s)
      Fujihara, Y., Murakami, M., Inoue, N., Satouh, Y., Kaseda, K., Ikawa, M., Okabe, M.
    • Journal Title

      Journal of cell science

      Volume: 123(Pt9) Pages: 1531-6

    • NAID

      120006957390

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and disruption of sperm-specific angiotensin converting enzyme-3 (ACE3) in mouse2010

    • Author(s)
      Inoue, N., Kasahara, T., Ikawa, M., Okabe, M.
    • Journal Title

      PloS One

      Volume: 5(4)

    • NAID

      120006957389

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] The mechanism of sperm-egg fusion in mouse and the involvement of IZUMO1 and a novel factor2011

    • Author(s)
      Inoue N
    • Organizer
      Gordon Research Conferences" Cell-Cell Fusion"
    • Place of Presentation
      USA
    • Year and Date
      2011-08-09
    • Related Report
      2011 Final Research Report
  • [Presentation] The Mechanism of Sperm-Egg Fusion in Mouse and the Involvement of IZUMO1 and a Novel Factor2011

    • Author(s)
      Naokazu Inoue
    • Organizer
      Gordon Research Conferences "Cell-Cell Fusion"
    • Place of Presentation
      University of New England, USA(招待講演)
    • Year and Date
      2011-08-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] ほ乳類の融合関連因子IZUMO1の構造と局在解析2010

    • Author(s)
      井上直和
    • Organizer
      第33回日本分子生物学会年会、ワークショップ
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 受精の膜融合における活性化メカニズム2010

    • Author(s)
      井上直和
    • Organizer
      大阪大学蛋白質研究所セミナー疾患と膜動態の蛋白質科学
    • Place of Presentation
      吹田市
    • Year and Date
      2010-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] 受精の暎融合における活性化メカニズム2010

    • Author(s)
      井上直和
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      大阪大学蛋白質研究所(招待講演)
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification and disruption of sperm-specific angiotensin converting enzyme-3(ACE3) in mouse2010

    • Author(s)
      Inoue N
    • Organizer
      International Symposium for Immunology of Reproduction joint meeting in conjunction with The 25^<th> Annual Meeting of Japan Society for Immunology of Reproduction
    • Place of Presentation
      Suita-city
    • Year and Date
      2010-08-28
    • Related Report
      2011 Final Research Report
  • [Presentation] A helix bundle involved in cell-cell fusion as shown by molecular dissection of sperm protein IZUMO12010

    • Author(s)
      Inoue N
    • Organizer
      11^<th> International Symposium on Spermatology
    • Place of Presentation
      Nago-city
    • Year and Date
      2010-06-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 受精の膜融合因子IZUMO1の膜融合活性化領域の同定とその構造解析2010

    • Author(s)
      井上直和
    • Organizer
      第57回日本実験動物学会
    • Place of Presentation
      京都市
    • Year and Date
      2010-05-12
    • Related Report
      2011 Final Research Report
  • [Presentation] 受精の膜融合因子IZUMO1の膜融合活性化領域の同定とその構造解析2009

    • Author(s)
      井上直和
    • Organizer
      第32回日本分子生物学会年会,ワークショップ
    • Place of Presentation
      横浜市
    • Year and Date
      2009-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 受精の膜融合因子Izumoの膜融合活性化領域の同定とその構造解析2009

    • Author(s)
      井上直和、浜田大三、上久保裕生、平田邦生、片岡幹雄、山本雅貴、伊川正人、岡部勝、萩原義久
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2009-12-10
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.egr.biken.osaka-u.ac.jp/index.php

    • Related Report
      2011 Final Research Report
  • [Remarks] MSN産経ニュース報道関係

    • URL

      http://sankei.jp.msn.com/science/news/120320/scn12032013550000-n1.htm

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.egr.biken.osaka-u.ac.jp/index.php

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://133.1.15.131/EGR/index.cfm

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://133.1.15.131/EGR/indexJ.cfm

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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