Elucidation of membrane fusion machinery in fertilization
Project/Area Number |
21687018
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Developmental biology
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Research Institution | Osaka University |
Principal Investigator |
INOUE Naokazu 大阪大学, 微生物病研究所, 助教 (50379096)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2010: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
|
Keywords | 受精 / 膜融合 / 精子 / 卵子 |
Research Abstract |
Despite the biological importance of sperm-egg fusion in fertilization, the molecular mechanisms underlying this step remain largely unknown. We focused on elucidating the molecular mechanisms of sperm-egg fusion by using genetically manipulated mice. In this application, we reached to obtain two aspects about mammalian fertilization as follows. We identified an IZUMO1-interacting protein in spermatozoon. We produced an Ace3 disrupted mouse line, and found the localization of IZUMO1 spread in a little wider area on spermatozoa, but the elimination of ACE3 did not result in a loss of sperm fertilizing ability. Utilizing Izumo1-deficient spermatozoa, we found that the spermatozoa had the ability to pass through the ZP at least twice. Apparently, some spermatozoa that had undergone the acrosome reaction long before contact with the ZP remained capable of crossing the ZP and fertilizing eggs. The concept that acrosome-reacted spermatozoa are unable to bind to the ZP and have lost their fertilizing capacity must be reconsidered.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Calsperin is a testis-specific chaperone required for sperm fertility.2011
Author(s)
Ikawa, M., Tokuhiro, K, Yamaguchi, R., Benham, A, M., Tamura, T., Wada, I., Satouh, Y., Inoue, N., Okabe, M.
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Journal Title
Journal of Biological Chemistry
Volume: 288(7)
Pages: 5639-46
NAID
Related Report
Peer Reviewed
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[Journal Article] The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection.2010
Author(s)
Oshiumi, H., Miyashita, M., Inoue, N., Okabe, M., Matsumoto, M., Seya, T.
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Journal Title
Cell Host Microbe
Volume: 8(6)
Pages: 496-509
NAID
Related Report
Peer Reviewed
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[Journal Article] Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse2010
Author(s)
Fujihara, Y., Murakami, M., Inoue, N., Satouh, Y., Kaseda, K., Ikawa, M., Okabe, M.
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Journal Title
Journal of cell science
Volume: 123(Pt9)
Pages: 1531-6
NAID
Related Report
Peer Reviewed
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