Functional Analysis of the Psyllid Bacteriome to Harbor Carsonella, Mutualistic Bacteria with an Extremely Reduced Genome

• Project/Area Number: 21687020
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: Toyohashi University of Technology (2011-2012); The Institute of Physical and Chemical Research (2009-2010)
• Principal Investigator: NAKABACHI Atsushi 豊橋技術科学大学, エレクトロニクス先端融合研究所, 准教授 (40332267)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000); Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2010: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000); Fiscal Year 2009: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
• Keywords: キジラミ / 共生 / 菌細胞 / カルソネラ / プロフテラ / 極小ゲノム / トランスクリプトーム解析 / ゲノム解析 / 二次共生細菌 / RNA干渉 / bacteriome / トランスクリプトーム / RNA-Seq / 新規生理活性物質 / 第二世代シーケンサー / 次世代シーケンサー

Research Abstract

Transcriptome analysis of the bacteriome of the two psyllid species, P. venustaand D. citrirevealed genes that apparently play pivotal roles to maintain either the psyllid-Carsonellasymbiosis or the psyllid-Carsonella-Profftellasymbiosis.RNAi analysis gave insight into the function of these genes. Whole genome analysis of Carsonella_DC and Profftellarevealed their reductive evolution, demonstrating that Profftellais an unprecedented type of defensive symbiont.

Research Products

• [Journal Article] Defensive bacteriome symbiont with a drastically reduced genome. (2013)
  • Author(s): Nakabachi A, UeokaR, OshimaK, TetaR, MangoniA, GurguiM, OldhamNJ, van Echten-DeckertG, OkamuraK, YamamotoK, InoueH, OhkumaM, HongohY, MiyagishimaSY, HattoriM, PielJ, FukatsuT
  • Journal Title: CurrBiol
  • Peer Reviewed
• [Journal Article] 新規防除法開発の標的としてのアブラムシ栄養共生系 (2012)
  • Author(s): 中鉢淳
  • Journal Title: 植物防疫 Volume: 66(2) Pages: 24-29
• [Journal Article] Genome size of Pachypsylla venusta(Hemiptera: Psyllidae), and the ploidy of its bacteriocyte, the symbiotic host cellthat harbors intracellular mutualistic bacteria with the smallest cellular genome (2010)
  • Author(s): Nakabachi A, Koshikawa S, Miura T, Miyagishima S
  • Journal Title: Bull Entomol Res Volume: 100(1) Pages: 27-33
  • Peer Reviewed
• [Journal Article] Genome sequence of the pea aphid Acyrthosiphon pisum (2010)
  • Author(s): The International Aphid Genomics Consortium
  • Journal Title: PLoS Biol Volume: 8(2)e1000313
  • Peer Reviewed
• [Journal Article] Bacterial genes in the aphid genome: Absence of functional gene transfer from Buchnerato its host (2010)
  • Author(s): Nikoh N, McCutcheon JP, Kudo T, Miyagishima S, Moran NA, Nakabachi A.
  • Journal Title: PLoS Genet Volume: 6(2):e1000827
  • Peer Reviewed
• [Journal Article] Expansion of genes encoding a novel type of dynamin in the genome of the pea aphid, Acyrthosiphon pisum (2010)
  • Author(s): Nakabachi A, Miyagishima S.
  • Journal Title: Insect Mol Biol Volume: 19(s2) Pages: 165-173
  • Peer Reviewed
• [Journal Article] Chitinase-like proteins encoded in the genome of the pea aphid, Acyrthosiphon pisum (2010)
  • Author(s): Nakabachi A, Shigenobu S, Miyagishima S.
  • Journal Title: Insect Mol Biol Volume: 19(s2) Pages: 175-185
  • Peer Reviewed
• [Journal Article] A full-length cDNA resource for the pea aphid, Acyrthosiphon pisum (2010)
  • Author(s): Shigenobu S,Richards S, Cree AG, Morioka M, Fukatsu T, Kudo T, Miyagishima S, Gibbs RA, Stern D, Nakabachi A
  • Journal Title: Insect Mol Biol Volume: 19(s2) Pages: 23-31
  • Peer Reviewed
• [Journal Article] A genomic analysis of transcytosis in the pea aphid, Acyrthosiphon pisum, a mechanism involved in virus transmission (2010)
  • Author(s): Tamborindeguy C,MonsionB, BraultV, HunnicuttL, Ju HJ, NakabachiA, Van FleetE.
  • Journal Title: Insect Mol Biol Volume: 19(s2) Pages: 259-272
  • Peer Reviewed
• [Journal Article] Immunity and other defenses in pea aphids, Acyrthosiphon pisum. (2010)
  • Author(s): GerardoNM, AltincicekB, AnselmeC, AtamianH, BarribeauSM, de VosM, Duncan EJ, EvansJD, GabaldonT, GhanimM, HeddiA, KaloshianI, LatorreA, MoyaA, NakabachiA, ParkerBJ, Perez-BrocalV, PignatelliM, RahbeY, RamseyJS, Spragg C, TamamesJ, TamaritD, TamborindeguyC, Vincent-Monegat C, VilcinskasA.
  • Journal Title: Genome Biol Volume: 11(2):R21
  • Peer Reviewed
• [Journal Article] Genomic evidence for complementary purine metabolism in the pea aphid, Acyrthosiphon pisum, and its symbiotic bacterium Buchnera aphidicola. (2010)
  • Author(s): RamseyJS, MacDonald SJ, Jander G, Nakabachi A, Thomas GH, Douglas AE
  • Journal Title: Insect Mol Biol Volume: 19(s2) Pages: 241-248
  • Peer Reviewed
• [Journal Article] Genome size of Pachypsylla venusta(Hemiptera : Psyllidae), and the ploidy of its bacteriocyte, the symbiotic host cell that harbors intracellular mutualistic bacteria with the smallest cellular genome (2010)
  • Author(s): Nakabachi A, Koshikawa S, Miura T, Miyagishima S
  • Journal Title: Bulletin of Entomological Research 100 Pages: 27-33
  • Peer Reviewed
• [Journal Article] Expansion of genes encoding a novel type of dynamin in the genome of the pea aphid, Acyrthosiphon pisum (2010)
  • Author(s): Nakabachi A, Miyagishima S
  • Journal Title: Insect Molecular Biology 19 Pages: 165-173
  • Peer Reviewed
• [Journal Article] Chitinase-like proteins encoded in the genome of the pea aphid, Acyrthosiphon pisum (2010)
  • Author(s): Nakabachi A, Shigenobu S, Miyagishima S
  • Journal Title: Insect Molecular Biology 19 Pages: 175-185
  • Peer Reviewed
• [Journal Article] A full-length cDNA resource for the pea aphid, Acyrthosiphon pisum (2010)
  • Author(s): Shigenobu S, Richards S, Cree AG, Morioka M, Fukatsu T, Kudo T, Miyagishima S, Gibbs RA, Stern D, Nakabachi A
  • Journal Title: Insect Molecular Biology 19 Pages: 23-31
  • Peer Reviewed
• [Journal Article] Bacterial genes in the aphid genome : Absence of functional gene transfer from Buchnera to its host (2010)
  • Author(s): Nikoh N, McCutcheon JP, Kudo T, Miyagishima S, Moran NA, Nakabachi A
  • Journal Title: PLoS Genetics 6
  • Peer Reviewed
• [Journal Article] Genome sequence of the pea aphid Acyrthosiphon pisum (2010)
  • Author(s): The International Aphid Genomics Consortium
  • Journal Title: PLoS Biology 8
  • Peer Reviewed
• [Journal Article] A genomic analysis of transcytosis in the pea aphid, Acyrthosiphon pisum, a mechanism involved in virus transmission (2010)
  • Author(s): Tamborindeguy C, Monsion B, Brault V, Hunnicutt L, Ju HJ, Nakabachi A, Van Fleet E
  • Journal Title: Insect Molecular Biology 19 Pages: 259-272
  • Peer Reviewed
• [Journal Article] Genomic evidence for complementary purine metabolism in the pea aphid, Acyrthosiphon pisum, and its symbiotic bacterium Buchnera aphidicola (2010)
  • Author(s): Ramsey JS, MacDonald SJ, Jander G, Nakabachi A, Thomas GH, Douglas AE
  • Journal Title: Insect Molecular Biology 19 Pages: 241-248
  • Peer Reviewed
• [Journal Article] Immunity and other defenses in pea aphids, Acyrthosiphon pisum (2010)
  • Author(s): Gerardo NM et al.
  • Journal Title: Genome Biology 11
  • Peer Reviewed
• [Presentation] Functions and evolution of a dual bacterial symbiosis in a serious citrus pest (2012)
  • Author(s): Nakabachi A, UeokaR, OshimaK, TetaR, MangoniA, GurguiM, OldhamNJ, van Echten-DeckertG, OkamuraK, YamamotoK, InoueH, OhkumaM, HongohY, MiyagishimaSY, HattoriM, PielJ, FukatsuT.
  • Organizer: 第28回日本微生物生態学会大会
  • Place of Presentation: 豊橋技術科学大学(豊橋)
  • Year and Date: 2012-09-20
• [Presentation] Aphids acquiredsymbiotic genes from bacteria (2012)
  • Author(s): Nakabachi A
  • Organizer: XXIV International Congress of Entomology
  • Place of Presentation: Daegu, Korea
  • Year and Date: 2012-08-20
• [Presentation] ミカンキジラミの防衛共生 (2012)
  • Author(s): 中鉢淳、上岡麗子、大島健志朗、Alfonso Mangoni、Mihaela Gurgui、Neil Oldham、Gerhild van Echten-Deckert、井上広光、大熊盛也、本郷裕一、宮城島進也、服部正平、Jorn Piel、深津武馬
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 近畿大学(奈良)
  • Year and Date: 2012-03-28
• [Presentation] ミカンキジラミ共生細菌2種のゲノム構造と機能的役割 (2012)
  • Author(s): 中鉢淳、大島健志朗、上岡麗子、Alfonso Mangoni、Mihaela Gurgui、Neil Oldham、Gerhild van Echten-Deckert、井上広光、大熊盛也、本郷裕一、宮城島進也、Jorn Piel、服部正平、深津武馬
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学(東京)
  • Year and Date: 2012-03-10
• [Presentation] Functions and evolution of a dual bacterial symbiosis in a serious citrus pest, Diaphorina citri (2012)
  • Author(s): A. Nakabachi, R. Ueoka, K. Oshima, A. Mangoni, M. Gurgui, N. Oldham, G. van Echten-Deckert, H. Inoue, M. Ohkuma, Y. Hongoh, S. Miyagishima, M. Hattori, J. Piel, T. Fukatsu
  • Organizer: 第 28 回日本微生物生態学会大会
  • Place of Presentation: 豊橋技術科学大学(愛知県豊橋市)
• [Presentation] The obligate symbiosis in aphids: apromising target for highly selective pest control (2011)
  • Author(s): Nakabachi A
  • Organizer: The Asia-Pacific Interdisciplinary Research Conference 2011
  • Place of Presentation: Toyohashi University of Technology (Toyohashi).
  • Year and Date: 2011-11-18
• [Presentation] The Obligate Symbiosis in Aphids : A Promising Target for Highly Selective Pest Control (2011)
  • Author(s): Atsushi Nakabachi
  • Organizer: The Asia-Pacific Interdisciplinary Research Conference 2011
  • Place of Presentation: Toyohashi University of Technology (Toyohashi)
  • Year and Date: 2011-11-18
• [Presentation] アブラムシゲノム上の細菌由来水平転移遺伝子は発現し、産物はBuchneraへ輸送される (2011)
  • Author(s): 中鉢淳
  • Organizer: 第55回日本応用動物昆虫学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-03-27
• [Presentation] ミカンキジラミ必須共生細菌2種のゲノム解析 (2011)
  • Author(s): 中鉢淳、大島健志朗、宮城島進也、服部正平、深津武馬
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 東北学院大学(仙台)
  • Year and Date: 2011-03-14
• [Presentation] ミカンキジラミ必須共生細菌2種のゲノム解析 (2011)
  • Author(s): 中鉢淳
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-14
• [Presentation] Aphids acquired symbiotic genes via lateral gene transfer : evidence at the protein level (2010)
  • Author(s): Atsushi Nakabachi
  • Organizer: Memorial Symposium for the 26th International Prize for Biology
  • Place of Presentation: つくば
  • Year and Date: 2010-12-07
• [Presentation] アブラムシは、かつて別の細菌から獲得した遺伝子で必須共生細菌を制御する (2010)
  • Author(s): 中鉢淳
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-25
• [Presentation] 宿主昆虫と必須共生細菌のゲノム進化 (2010)
  • Author(s): 中鉢淳
  • Organizer: 日本進化学会大会第12回大会
  • Place of Presentation: 東京工業大学(東京)
  • Year and Date: 2010-08-03
• [Presentation] 宿主昆虫と必須共生細菌のゲノム進化 (2010)
  • Author(s): 中鉢淳
  • Organizer: 日本進化学会大会第12回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-08-03
• [Presentation] アブラムシはボルバキアから得た遺伝子を利用してブフネラを制御する? (2010)
  • Author(s): 中鉢淳、John McCutcheon、工藤俊章、宮城島進也、Nancy Moran、二河成男
  • Organizer: 第54回日本応用動物昆虫学会大会
  • Place of Presentation: 千葉大学・西千葉キャンパス(千葉市)
  • Year and Date: 2010-03-27
• [Presentation] アブラムシゲノムにコードされ、共生器官で高発現する細菌由来水平転移遺伝子群 (2010)
  • Author(s): 中鉢淳、John McCutcheon、工藤俊章、宮城島進也、Nancy Moran、二河成男
  • Organizer: 第4回日本ゲノム微生物学会年会
  • Place of Presentation: 九州大学医学部百年講堂(福岡市)
  • Year and Date: 2010-03-08
• [Remarks]
  • URL: http://www.eiiris.tut.ac.jp/nakabachi.html
• [Remarks]
  • URL: http://www.riken.jp/r-world/info/release/press/2010/100223_2/