The biological role of odorant-binding proteins in insect behavior

• Project/Area Number: 21688003
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: The University of Tokyo (2011); Tokyo Metropolitan University (2009-2010)
• Principal Investigator: MATSUO Takashi 東京大学, 大学院・農学生命科学研究科, 准教授 (70301223)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
• Keywords: 昆虫分子生物学 / 化学感覚 / 進化 / 食性 / ショウジョウバエ / OBP

Research Abstract

Biological roles of odorant-binding proteins were studied at the multiple levels. At the molecular level, synthesized OBP57d and OBP57e from Drosophila melanogaster showed highest affinity to tridecanoic acid in the in vitro binding assay. At the behavioral level, D. melanogaster showed avoidance to tridecanoic acid in the oviposition-site selection assay. These results proved a link between the molecular function and the biological role of OBPs.

Research Products

• [Journal Article] Contribution of olfactory and gustatory sensations of octanoic acid in the oviposition behavior of Drosophila melanogaster(Diptera : Drosophilidae) (2012)
  • Author(s): Matsuo T
  • Journal Title: Applied Entomology and Zoology Volume: 47 Issue: 2 Pages: 137-142
  • DOI: 10.1007/s13355-012-0100-3
  • NAID: 40019278575
  • Peer Reviewed
• [Journal Article] Functional Evolution of Duplicated Odorant-Binding Protein Genes, Obp57d and Obp57e, in Drosophila (2012)
  • Author(s): Eriko Harada
  • Journal Title: PLoS ONE Volume: 7(1) Issue: 1 Pages: e29710-e29710
  • DOI: 10.1371/journal.pone.0029710
  • Peer Reviewed
• [Journal Article] Two types of cis-trans compensation in the evolution of transcriptional regulation (2011)
  • Author(s): Takahashi KR, Matsuo T, Takano-Shimizu-Kouno T.
  • Journal Title: Proceedings of National Academy of Science USA Volume: 108 Issue: 37 Pages: 15276-15281
  • DOI: 10.1073/pnas.1105814108
  • Peer Reviewed
• [Journal Article] Identification of odorant-binding protein genes from antennal expressed sequence tags of the onion fly, Delia antiqua (2011)
  • Author(s): Mitaka H, Matsuo T, Miura N, Ishikawa Y.
  • Journal Title: Molecular Biology Reports Volume: 38 Issue: 3 Pages: 1787-1792
  • DOI: 10.1007/s11033-010-0293-x
  • Peer Reviewed
• [Journal Article] Evolution of expression patterns of two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila (2010)
  • Author(s): Yasukawa J, Tomioka S, Aigaki T, Matsuo T
  • Journal Title: Gene Volume: 467 Issue: 1-2 Pages: 25-34
  • DOI: 10.1016/j.gene.2010.07.006
  • Peer Reviewed
• [Journal Article] The shaping of male courtship posture by lateralized gustatory inputs to male-specific interneurons (2010)
  • Author(s): Koganezawa M, Haba D, Matsuo T, Yamamoto D
  • Journal Title: Current Biology Volume: 20 Issue: 1 Pages: 1-8
  • DOI: 10.1016/j.cub.2009.11.038
  • Peer Reviewed
• [Journal Article] ショウジョウバエの食性の進化と行動 (2010)
  • Author(s): 松尾隆嗣
  • Journal Title: 昆虫と自然 Volume: 45 Pages: 12-15
• [Journal Article] The shaping of male courtship posture by lateralized gustatory inputs to male-specific interneurons. (2010)
  • Author(s): Koganezawa, M., et al.
  • Journal Title: Current Biology 20 Pages: 1-8
  • Peer Reviewed
• [Journal Article] ショウジョウバエの食性の進化と行動 (2010)
  • Author(s): 松尾隆嗣
  • Journal Title: 昆虫と自然 45 Pages: 12-15
• [Journal Article] ショウジョウバエの食性進化と化学感覚受容 (2009)
  • Author(s): 松尾隆嗣
  • Journal Title: 生物科学 Volume: 61 Pages: 24-34
• [Journal Article] ショウジョウバエの食性進化と化学感覚受容 (2009)
  • Author(s): 松尾隆嗣
  • Journal Title: 生物科学 61 Pages: 24-31
  • Peer Reviewed
• [Presentation] ショウジョウバエの食性と匂い物質結合蛋白質の進化 (2012)
  • Author(s): 松尾隆嗣
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 信州大学(長野県)(招待講演)
  • Year and Date: 2012-03-31
• [Presentation] ショウジョウバエ近縁種間における食性多様化の遺伝的メカニズム (2012)
  • Author(s): 松尾隆嗣
  • Organizer: 第59回日本生態学会大会
  • Place of Presentation: 龍谷大学(滋賀県)(招待講演)
  • Year and Date: 2012-03-20
• [Presentation] ショウジョウバエの食性と匂い物質結合蛋白質の進化 (2011)
  • Author(s): 松尾隆嗣
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都大学(京都府)(招待講演)
  • Year and Date: 2011-09-20
• [Presentation] Evolution of two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila (2011)
  • Author(s): Matsuo T.
  • Organizer: International Congress of Comparative Physiology and Biochemistry 2011
  • Place of Presentation: Nagoya
  • Year and Date: 2011-06-01
• [Presentation] Evolution of two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila (2011)
  • Author(s): Takashi Matsuo
  • Organizer: International Congress of Comparative Physiology and Biochemistry 2011
  • Place of Presentation: 名古屋国際会議場(愛知県)(招待講演)
  • Year and Date: 2011-06-01
• [Presentation] Evolution of two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila (2010)
  • Author(s): Matsuo T.
  • Organizer: Beijing International Symposium on Cellular and Molecular Mechanisms of Chemoreception
  • Place of Presentation: Beijing
  • Year and Date: 2010-09-03
• [Presentation] Evolution of two odorant-binding protein genes, Obp57d and Ohp57e, in Drosophila (2010)
  • Author(s): Takashi Matsuo
  • Organizer: Beijing international symposium on cellular and molecular mechanisms of chemoreception
  • Place of Presentation: China Agricultural University, Beijing, China
• [Presentation] Genetic analysis of two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila ; their evolution and biological roles (2009)
  • Author(s): Matsuo T.
  • Organizer: Fifth Asia Pacific Conference on Chemical Ecology
  • Place of Presentation: Hawaii
  • Year and Date: 2009-10-28
• [Presentation] Genetic analysis of two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila ; their evolution and biological roles. (2009)
  • Author(s): Matsuo, T.
  • Organizer: Fifth Asia Pacific Conference on Chemical Ecology
  • Place of Presentation: Hawaii
  • Year and Date: 2009-10-28
• [Presentation] Rapid evolution of two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila. (2009)
  • Author(s): Matsuo, T.
  • Organizer: Association for Chemoreception Sciences 31st annual meeting
  • Place of Presentation: Florida
  • Year and Date: 2009-04-24
• [Presentation] Rapid evolution of two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila (2009)
  • Author(s): Matsuo T.
  • Organizer: Association for Chemoreception Sciences 31st annual meeting
  • Place of Presentation: Florida
  • Year and Date: 2009-02-24
• [Remarks]
  • URL: http://sites.google.com/site/matzoresearch/
• [Remarks]
  • URL: http://sites.google.com/site/matzoresearch/
• [Remarks]
  • URL: http://sites.google.com/site/matzoresearch/
• [Remarks]
  • URL: http://fly.biol.se.tmu.ac.jp/