Molecular background underlying reviving step of anhydrobiosis

• Project/Area Number: 21688004
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: KIKAWADA Takahiro 独立行政法人農業生物資源研究所, 昆虫機能研究開発ユニット, 主任研究員 (60414900)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥27,040,000 (Direct Cost: ¥20,800,000、Indirect Cost: ¥6,240,000); Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
• Keywords: 昆虫 / ストレス / 修復 / 遺伝子 / 乾燥耐性 / 生体分子修復 / 乾燥休眠 / トレハロース / 網羅的解析 / ESTデータベース / 吸水過程

Research Abstract

We investigated molecular mechanisms underlying reparation systems during rehydration process of anhydrobiosis in the sleeping chironomid. From microarray analysis, expressions of over 400 genes were specifically up-regulated in the process of rehydration. The process of desiccation and rehydration in anhydrobiosis should cause serious oxidative stress in the sleeping chironomid, so that their genomic DNAs and proteins must be injured during anhydrobiosis. During rehydration, expression of reparation enzymes for DNA and proteins, such as Rad51 and protein L-isoaspartyl methyltransferase (PIMT), may lead to successful resurrection from desiccated form

Research Products

• [Journal Article] Wide overlap in mRNA transcription response to desiccation or oxidative stress in larvae of the cryptobiotic insect (2012)
  • Author(s): Mukae K, Gusev O, Hatanaka R, Cornette R, Okuda T, Kikawada T
  • Journal Title: Cryobiol Cryotech Volume: 58 Pages: 191-194
  • NAID: 110009612936
  • Peer Reviewed
• [Journal Article] Elucidation of mechanisms underlying desiccation tolerance with exhaustive gene analysis (2012)
  • Author(s): Mukae K, Cornette R, Gusev O, Hatanaka R, Okuda T, Kikawada T
  • Journal Title: Cryobiol Cryotech Volume: 58 Pages: 73-76
  • NAID: 110009457812
  • Peer Reviewed
• [Journal Article] 極限環境に生きる昆虫ネムリユスリカの生存戦略 (2012)
  • Author(s): 菊田真吾, 畑中理恵, 黄川田隆洋
  • Journal Title: 生物科学 Volume: 63 Pages: 195-204
• [Journal Article] Elucidation of mechanisms underlying desiccation tolerance with exhaustive gene analysis (2012)
  • Author(s): Mukae K, Cornette R, Gusev O, Hatanaka R, Okuda T, Kikawada T
  • Journal Title: 低温生物工学会誌 Volume: 58 Pages: 73-76
  • NAID: 110009457812
  • Peer Reviewed
• [Journal Article] Wide overlap in mRNA transcription response to desiccation or oxidative stress in larvae of the cryptobiotic insect (2012)
  • Author(s): Mukae K, Gusev O, Hatanaka R, Cornette R, Okuda T, Kikawada T
  • Journal Title: 低温生物工学会誌 Volume: 58 Pages: 191-194
  • NAID: 110009612936
  • Peer Reviewed
• [Journal Article] Effects of Group 3 LEA protein model peptides on desiccation-induced protein aggregation (2012)
  • Author(s): Furuki T, Shimizu T, Chakrabortee S, Yamakawa K, Hatanaka R, Takahashi T, Kikawada T, Okuda T, Mihara H, Tunnacliffe A, Sakurai M
  • Journal Title: Biochimica et Biophysica Acta-Proteins & Proteomics Volume: 1824 Issue: 7 Pages: 891-897
  • DOI: 10.1016/j.bbapap.2012.04.013
  • Peer Reviewed
• [Journal Article] The induction of anhydrobiosis in the sleeping chironomid: Current status of our knowledge (2011)
  • Author(s): Cornette R, Kikawada T
  • Journal Title: IUBMB Life Volume: 63 Issue: 6 Pages: 419-429
  • DOI: 10.1002/iub.463
  • Peer Reviewed
• [Journal Article] ネムリユスリカLEAタンパク質(PvLEA4)の機能解析 (2011)
  • Author(s): 畑中理恵,薦田(萩原)優香,金森保志,藤田弥佳,コルネット・リシャー,奥田隆,黄川田隆洋
  • Journal Title: Cryobiol Cryotech Volume: 57 Pages: 135-138
  • NAID: 110009457796
  • Peer Reviewed
• [Journal Article] Mechanisms underpinning induction of anhydrobiosis in Polypedilum vanderplanki (2011)
  • Author(s): Kikawada T
  • Journal Title: Cryobiol Cryotech Volume: 57 Pages: 33-43
• [Journal Article] ネムリユスリカ由来の細胞保護タンパク室の機能と低分子ペプチドによる代替 (2011)
  • Author(s): 畑中理恵,古木隆生,櫻井実,黄川田隆
  • Journal Title: バイオインダストリー Volume: 28 Pages: 43-48
• [Journal Article] Expression of heat shock protein-coding genes associated with anhydrobiosis in an African chironomid Polypedilum vanderplanki (2011)
  • Author(s): Gusev O, Cornette R, Kikawada T, Okuda T
  • Journal Title: Cell Stress Chaperones Volume: 16 Issue: 1 Pages: 81-90
  • DOI: 10.1007/s12192-010-0223-9
  • Peer Reviewed
• [Journal Article] Salt effects on the structural and thermodynamic properties of a group 3 LEA protein model peptide (2011)
  • Author(s): Furuki T, Shimizu T, Kikawada T, Okuda T, Sakurai M
  • Journal Title: Biochemistry Volume: 50 Issue: 33 Pages: 7093-7103
  • DOI: 10.1021/bi200719s
  • Peer Reviewed
• [Journal Article] グループ3LEAタンパク質の22-merモデルペプチドが示すタンパク質凝集抑制効果 (2011)
  • Author(s): 古木隆生, 清水天平, Chakraborte S, 畑中理恵, 高橋剛, 黄川田隆洋, 奥田隆, 三原久和, Tunnacliffe A, 櫻井実
  • Journal Title: 低温生物工学会誌 Volume: 57 Pages: 139-142
  • NAID: 110009457797
  • Peer Reviewed
• [Journal Article] ネムリユスリカLEAタンパク質(PvLEA4)の機能解析 (2011)
  • Author(s): 畑中理恵, 薦田(萩原)優香, 金森保志, 藤田弥佳, コルネット・リシャー, 奥田隆, 黄川田隆洋
  • Journal Title: 低温生物工学会誌 Volume: 57 Pages: 135-138
  • NAID: 110009457796
  • Peer Reviewed
• [Journal Article] Mechanisms underpinning induction of anhydrobiosis in Polypedilum vanderplanki (2011)
  • Author(s): Kikawada T
  • Journal Title: 低温生物工学会誌 Volume: 57 Pages: 33-43
• [Journal Article] Expression of heat shock protein-coding genes associated with anhydrobiosis in an African chironomid Polypedilum vanderplanki (2011)
  • Author(s): Gusev O, Cornette R, Kikawada T, Okuda T
  • Journal Title: Cell Stress & Chaperones Volume: 16 Pages: 81-90
  • Peer Reviewed
• [Journal Article] Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki (2010)
  • Author(s): Mitsumasu K, Kanamori Y, Fujita M, Iwata K, Tanaka D, Kikuta S, Watanabe M, Cornette R, Okuda T, Kikawada T
  • Journal Title: FEBS J Volume: 277 Issue: 20 Pages: 4215-4228
  • DOI: 10.1111/j.1742-4658.2010.07811.x
  • Year and Date: 2010-09-06
  • Peer Reviewed
• [Journal Article] Anhydrobiosis-associated nuclear DNA damage and repair in the sleeping chironomid: Linkage with radioresistance (2010)
  • Author(s): Gusev O, Nakahara Y, Vanyagina V, Malutina L, Cornette R, Sakashita T, Hamada N, Kikawada T, Kobayashi Y, Okuda T
  • Journal Title: PLoS ONE Volume: 5 Issue: 11 Pages: 14008-14008
  • DOI: 10.1371/journal.pone.0014008
  • Peer Reviewed
• [Journal Article] Identification of anhydrobiosis-related genes from an expressed sequence tag database in the cryptobiotic midge Polypedilum vanderplanki (diptera; chironomidae) (2010)
  • Author(s): Cornette R, Kanamori Y, Watanabe M, Nakahara Y, Gusev O, Mitsumasu K, Kadono-Okuda K, Shimomura M, Mita K, Kikawada T, Okuda T
  • Journal Title: J Biol Chem Volume: 285 Issue: 46 Pages: 35889-35899
  • DOI: 10.1074/jbc.m110.150623
  • Peer Reviewed
• [Journal Article] ネムリユスリカの極限乾燥耐性(アンヒドロビオシス) (2010)
  • Author(s): コルネット・リシャー,黄川田隆洋
  • Journal Title: バイオサイエンスとインダストリー Volume: 68 Pages: 178-184
  • NAID: 10026195120
  • Peer Reviewed
• [Journal Article] Cells from an anhydrobiotic chironomid survive almost complete desiccation. (2010)
  • Author(s): Nakahara Y, Imanishi S, Mitsumasu K, Kanamori Y, Iwata K, Watanabe M, Kikawada T, Okuda T
  • Journal Title: Cryobiology Volume: 60 Issue: 2 Pages: 138-146
  • DOI: 10.1016/j.cryobiol.2009.10.004
  • Peer Reviewed
• [Journal Article] Anhydrobiosis-associated nuclear DNA damage and repair in the sleeping chironomid : Linkage with radioresistance (2010)
  • Author(s): Gusev O, Nakahara Y, Vanyagina V, Malutina L, Cornette R, Sakashita T, Hamada N, Kikawada T, Kobayashi Y, Okuda T
  • Journal Title: PLoS ONE Volume: 5
  • Peer Reviewed
• [Journal Article] Identification of anhydrobiosis-related genes from an expressed sequence tag database in the cryptobiotic midge Polypedilum vanderplanki (diptera ; chironomidae) (2010)
  • Author(s): Cornette R, Kanamori Y, Watanabe M, Nakahara Y, Gusev O, Mitsumasu K, Kadono-Okuda K, Shimomura M, Mita K, Kikawada T, Okuda T
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 35889-35899
  • Peer Reviewed
• [Journal Article] The effect of trehalose on the properties of mutant γPKC, which causes spinocerebellar ataxia type 14 (SCA14), in neuronal cell lines and cultured Purkinje cells (2010)
  • Author(s): Seki T, Abe-Seki N, Kikawada T, Takahashi H, Yamamoto K, Adachi N, Tanaka S, Hide I, Saito N, Sakai N
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 33252-33264
  • Peer Reviewed
• [Journal Article] Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki (2010)
  • Author(s): Mitsumasu K, Kanamori Y, Fujito M, Iwata K, Tanaka D, Kikuto S, Watanabe M, Cornette R, Okuda T, Kikawada T
  • Journal Title: FEBS Journal Volume: 277 Pages: 4215-4228
  • Peer Reviewed
• [Journal Article] Sugar transporter genes of the brown planthopper, Nilaparvata lugens : A facilitated glucose/fructose transporter (2010)
  • Author(s): Kikuta S, Kikawada T, Hagiwara-Komoda Y, Nakashima N, Noda H
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 40 Pages: 805-813
  • Peer Reviewed
• [Journal Article] The Trehalose transporter 1 gene sequence is conserved in insects and encodes proteins with different kinetic properties involved in trehalose import into peripheral tissues (2010)
  • Author(s): Kanamori Y, Saito A, Hagiwara-Komoda Y, Tanaka D, Mitsumasu K, Kikuta S, Watanabe M, Cornette R, Kikawada T, Okuda T
  • Journal Title: Insect Biochemistry and Molecular Biology 40 Pages: 30-37
  • Peer Reviewed
• [Journal Article] Cells from an anhydrobiotic chironomid survive almost complete desiccation (2010)
  • Author(s): Nakahara Y, Imanishi S, Mitsumasu K, Kanamori Y, Iwata K, Watanabe M, Kikawada T, Okuda T
  • Journal Title: Cryobiology 60 Pages: 138-146
  • Peer Reviewed
• [Journal Article] Desiccation-induced structuralization and glass formation of Group 3 late embryogenesis abundant protein model peptides (2010)
  • Author(s): Shimizu T, Kanamori Y, Furuki T, Kikawada T, Okuda T, Takahashi T, Mihara H, Sakurai M
  • Journal Title: Biochemistry 49 Pages: 1093-1104
  • Peer Reviewed
• [Journal Article] ネムリユスリカの乾燥耐性-アンヒドロビオシス-の分子機構 (2009)
  • Author(s): 黄川田隆洋
  • Journal Title: 比較内分泌学 Volume: 35 Pages: 98-106
  • NAID: 10024884417
• [Journal Article] ネムリユスリカの乾燥耐性-アンヒドロビオシス-の分子機構 (2009)
  • Author(s): 黄川田隆洋
  • Journal Title: 比較内分泌学 35 Pages: 98-106
  • NAID: 10024884417
• [Presentation] ネムリユスリカのゲノム解析から見えてきた乾燥無代謝休眠の分子機構 (2013)
  • Author(s): 黄川田隆洋,Gusev O Cornette R,末次克行
  • Organizer: 第57回日本応用動物昆虫学会大会 (招待講演)
  • Place of Presentation: 藤沢、神奈川
  • Year and Date: 2013-03-29
• [Presentation] ネムリユスリカの乾燥無代謝休眠を支えるゲノム情 (2012)
  • Author(s): 黄川田隆洋,グセフオレグ,コルネットリシャー,末次克行
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] Comparative genomics for understanding how to survive under extreme desiccation conditions (2012)
  • Author(s): Kikawada T, Gusev O, Suetsugu Y, Cornette R, Okuda T
  • Organizer: The Third International Scientific and Practical Conference: Postgenomic Methods of Analysis in Biology, and Laboratory and Clinical Medicine (招待講演)
  • Place of Presentation: カザニ、ロシア
  • Year and Date: 2012-11-24
• [Presentation] メタボローム解析からみたネムリユスリカの乾燥耐性におけるトレハロースの役割 (2012)
  • Author(s): 黄川田隆洋
  • Organizer: 第16回トレハロースシンポジウム (招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2012-10-25
• [Presentation] ネムリユスリカの極限乾燥耐性機構の分子基盤 (2012)
  • Author(s): 黄川田隆洋
  • Organizer: 第57回低温生物工学会年会 (招待講演)
  • Place of Presentation: つくば
  • Year and Date: 2012-06-01
• [Presentation] The sleeping chironomid genome project toward understanding molecular context underlying the extreme desiccation tolerance, anhydrobiosis (2012)
  • Author(s): Kikawada T, Gusev O, Cornette R, Okuda T
  • Organizer: International symposium of NODAI genome research center, Genome Research, Current challenges and Future directions (招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2012-01-21
• [Presentation] The sleeping chironomid genome project toward understanding molecular context underlying the extreme desiccation tolerance, anhydrobiosis (2012)
  • Author(s): Kikawada T, Gusev O, Cornette R, Okuda T
  • Organizer: International symposium of NODAI genome research center, Genome Research, Current challenges and Future directions
  • Place of Presentation: 東京品川コクヨホール(招待講演)
  • Year and Date: 2012-01-21
• [Presentation] Molecular mechanisms of anhydrobiosis in larvae of the African midge Polypedilum vanderplanki (2012)
  • Author(s): Cornette R, Kikawada T, Okuda T
  • Organizer: 6th International Workshop on Desiccation Sensitivity and Tolerance in Seeds and Vegetative Plant Tissues
  • Place of Presentation: Cradle of Humankind, South Africa
  • Year and Date: 2012-01-09
• [Presentation] Mecanisme de l' anhydrobiose chez la larve du Chironome africain Polypedilum vanderplanki (2011)
  • Author(s): Cornette R, Kikawada T, Okuda T
  • Organizer: Journee Francophone de la Recherche 2011
  • Place of Presentation: 東京日仏会館
  • Year and Date: 2011-12-17
• [Presentation] 極限乾燥耐性昆虫ネムリユスリカのLEAタンパク質は乾燥時に抗凝集活性を示す (2011)
  • Author(s): 畑中理恵, 薦田(萩原)優香, 金森保志, 藤田弥佳, 奥田隆, 黄川田隆洋
  • Organizer: 第12回極限環境生物学会年会
  • Place of Presentation: 長崎大学
  • Year and Date: 2011-11-27
• [Presentation] ネムリユスリカの乾燥耐性特異的遺伝子を導入したキイロショウジョウバエにおける耐性能力検証の試み (2011)
  • Author(s): 金森保志, 山田博万,渡邊和代, コルネット・リシャー, 黄川田隆洋, 奥田隆, 山本雅敏
  • Organizer: 第12回極限環境生物学会年会
  • Place of Presentation: 長崎大学
  • Year and Date: 2011-11-27
• [Presentation] 乾燥・放射線ストレスによる活性酸素種除去機構の差異 (2011)
  • Author(s): 迎恭輔, コルネット・リシャー, グセフ・オレグ, 奥田隆, 黄川田隆洋
  • Organizer: 第12回極限環境生物学会年会
  • Place of Presentation: 長崎大学
  • Year and Date: 2011-11-27
• [Presentation] 昆虫の排泄器官における糖の再吸収に関与するトランスポーターの同定 (2011)
  • Author(s): 菊田真吾, 萩原(薦田)優香, 野田博明, 黄川田隆洋
  • Organizer: 日本農芸化学会関東支部2011年度大会
  • Place of Presentation: 群馬県邑楽郡板倉町東洋大学生命科学部
  • Year and Date: 2011-10-15
• [Presentation] Anhydrobiomics research of the sleeping chironomid: toward a comprehensive understanding the molecular context underlying extreme desiccation tolerance (2011)
  • Author(s): Kikawada T, Cornette R, Gusev O, Suetsugu Y, Mukae K, Kikuta S, Hatanaka R, Shimura S, Satoh N, Okuda T
  • Organizer: 4th International Symposium of Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: レンヌ、フランス
  • Year and Date: 2011-07-18
• [Presentation] Adaptation to extreme environments and small genome size in Chironomids (2011)
  • Author(s): Comette R, Gusev O, Nakahara Y, Shimura S, Kikawada T, Okuda T
  • Organizer: 4th International Symposium of Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: Rennes, France
  • Year and Date: 2011-07-18
• [Presentation] Effects of Group 3 LEA model peptides on desiccation-induced protein aggregation (2011)
  • Author(s): Fukui T, Shimizu T, Chakrabortee S, Hatanaka R, Kikawada T, Okuda T, Tunnacliffe A, Sakurai M
  • Organizer: 4th International Symposium of Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: Rennes, France
  • Year and Date: 2011-07-18
• [Presentation] DNA damage and repair upon anhydrobiosis in the Sleeping Chironomid, Polypedilum vanderplanki (2011)
  • Author(s): Gusev O, Nakahara Y, Cornette R, Iwata K, Sakashita T, Hamada N, Kikawada T, Kobayashi Y, Majima H, Okuda T
  • Organizer: 4th International Symposium of Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: Rennes, France
  • Year and Date: 2011-07-18
• [Presentation] Biochemical characterization of the chironomid LEA protein, PvLEA4 (2011)
  • Author(s): Hatanaka R, Hagiwara-Komoda Y, Kanamori Y, Fujita M, Cornette R, Okuda T, Kikawada T
  • Organizer: 4th International Symposium of Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: Rennes, France
  • Year and Date: 2011-07-18
• [Presentation] Anhydrobiomics research of the sleeping chironomid : toward a comprehensive understanding the molecular context underlying extreme desiccation tolerance (2011)
  • Author(s): Kikawada T, Cornette R, Gusev O, Suetsugu Y, Mukae K, Kikuta S, Hatanaka R, Shimura S, Satoh N, Okuda T
  • Organizer: 4th International Symposium of Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: Rennes, France
  • Year and Date: 2011-07-18
• [Presentation] 極限乾燥耐性の分子メカニズム理解に向けたネムリユスリカanhydrobiomics. (2011)
  • Author(s): 黄川田隆洋,Cornette R, Gusev O,末次克行,菊田真吾,迎恭輔,畑中理恵,志村幸子,佐藤矩行,奥田隆
  • Organizer: 第56回低温生物工学会年会セミナー及び年会
  • Place of Presentation: 盛岡
  • Year and Date: 2011-07-08
• [Presentation] グループ3LEAタンパク質の22merモデルペプチドが示すタンパク質凝集抑制効果 (2011)
  • Author(s): 古木隆生, 清水天平, Chakraborte S, 高橋剛, 畑中理恵, 黄川田隆洋, 三原久和, 奥田隆, Tunnacliffe A, 櫻井実
  • Organizer: 第56回低温生物工学会年会セミナー及び年会
  • Place of Presentation: 盛岡市いわて県民情報交流センター
  • Year and Date: 2011-07-08
• [Presentation] ネムリユスリカLEAタンパク質(PvLEA4)の機能解析 (2011)
  • Author(s): 畑中理恵, 薦田(萩原)優香, 金森保志, 藤田弥佳, コルネット・リシャー, 奥田隆, 黄川田隆洋
  • Organizer: 第56回低温生物工学会年会セミナー及び年会
  • Place of Presentation: 盛岡市いわて県民情報交流センター
  • Year and Date: 2011-07-08
• [Presentation] 極限乾燥耐性の分子メカニズム理解に向けたネムリユスリカanhydrobiomics (2011)
  • Author(s): 黄川田隆洋, Cornette R, Gusev O, 末次克行, 菊田真吾, 迎恭輔, 畑中理恵, 志村幸子, 佐藤矩行, 奥田隆
  • Organizer: 第56回低温生物工学会年会セミナー及び年会
  • Place of Presentation: 盛岡市いわて県民情報交流センター
  • Year and Date: 2011-07-08
• [Presentation] マイクロアレイを用いたネムリユスリカにおける乾燥耐性機構の解析 (2011)
  • Author(s): 迎恭輔, コルネット・リシャー, オレグ・グセフ, 畑中理恵, 奥田隆, 黄川田隆洋
  • Organizer: 第56回低温生物工学会年会セミナー及び年会
  • Place of Presentation: 盛岡市いわて県民情報交流センター
  • Year and Date: 2011-07-08
• [Presentation] ヌマエラビル(Ozobranchus jantseanus)における耐凍性 (2011)
  • Author(s): 鈴木大, 宮本智子, 黄川田隆洋, 渡辺学, 鈴木徹
  • Organizer: 第56回低温生物工学会年会セミナー及び年会
  • Place of Presentation: 盛岡市いわて県民情報交流センター
  • Year and Date: 2011-07-08
• [Presentation] ユスリカにおける極限環境適応の進化:系統関係とゲノムサイズの影響 (2011)
  • Author(s): Cornette R, Gusev O, 中原雄一, 黄川田隆洋, 奥田隆
  • Organizer: 第22回ユスリカ研究集会
  • Place of Presentation: 滋賀県近江八幡市「休暇村近江八幡」
  • Year and Date: 2011-05-08
• [Presentation] ネムリユスリカの極限乾燥耐性(anhydrobiosis)を支える分子機構 (2011)
  • Author(s): 黄川田隆洋
  • Organizer: 日本農芸化学会2011年度大会 (招待講演)
  • Place of Presentation: 京都
  • Year and Date: 2011-03-25
• [Presentation] ネムリユスリカの極限乾燥耐性(anhydrobiosis)を支える分子機構 (2011)
  • Author(s): 黄川田隆洋
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-25
• [Presentation] トビイロウンカから同定されたプロトン-トレハロースシンポーターNIST8は生体内の恒常性の維持に関与する (2011)
  • Author(s): 菊田真吾, 萩原優香, 黄川田隆洋, 野田博明
  • Organizer: 第55回日本応用動物昆虫学会大会
  • Place of Presentation: 九州大学
• [Presentation] ネムリユスリカの極限乾燥耐性機構 (2010)
  • Author(s): 黄川田隆洋
  • Organizer: 47th CRCSeminar (招待講演)
  • Place of Presentation: 盛岡
  • Year and Date: 2010-12-16
• [Presentation] ネムリユスリカの極限乾燥耐性機構 (2010)
  • Author(s): 黄川田隆洋
  • Organizer: 47th CRC Seminar
  • Place of Presentation: 岩手大学
  • Year and Date: 2010-12-16
• [Presentation] ネムリユスリカの極限乾燥耐性:LEAタンパク質(PvLEA4)の機能解析 (2010)
  • Author(s): 畑中理恵, 薦田(萩原)優香, 金森保志, 藤田弥佳, コルネット・リシャー, 奥田隆, 黄川田隆洋
  • Organizer: 第11回極限環境生物学会年会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-11-16
• [Presentation] ネムリユスリカにおける乾燥耐性関連遺伝子の網羅的解析 (2010)
  • Author(s): 迎恭輔, コルネット・リシャー, オレグ・グセフ, 畑中恵理, 奥田隆, 黄川田隆洋
  • Organizer: 第11回極限環塊生物学会年会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-11-16
• [Presentation] Identification of anhydrobiosis-related genes from an EST database in the sleeping chironomid Polypedilum vanderplanki (2010)
  • Author(s): Cornette R, Kanamori Y, Gusev O, Nakahara Y, Fujita A, Watanabe M, Kikawada T, Okuda T
  • Organizer: 17th International SPACC Symposium
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2010-10-14
• [Presentation] Protection of genome of Polypedilum vanderplanki againsts DNA damage caused by desiccation and different types of radiation (2010)
  • Author(s): Gusev O, Cornette R, Nakahara Y, Kikawada T, Okuda T
  • Organizer: 17th International SPACC Symposium
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2010-10-14
• [Presentation] The sleeping chironomid : An insect survived 18 months of exposure to outer space (2010)
  • Author(s): Gusev O, Sakashita T, Sychev V, Novikova N, Sugimoto M, Malyutina L, Kikawada T, Okuda T
  • Organizer: 38th COSPAR Scientifrc Assembly
  • Place of Presentation: ブレーメン(ドイツ)
  • Year and Date: 2010-07-20
• [Presentation] Identification and characterization of a newly synthesized protein in suspension-cultured cells of the liverwort Marchantia polymorpha during acquisition of desiccation tolerance (2010)
  • Author(s): Hatanaka R, Furuki T, Shimizu T, Kikawada T, Takezawa D, Sugawara Y
  • Organizer: Cryobiology 2010
  • Place of Presentation: ブリストル(イギリス)
  • Year and Date: 2010-07-19
• [Presentation] Molecular cloning and characterizaton of a novel LEA protein in an anhydrobiosis insect, Polypedilum vanderplanki (2010)
  • Author(s): Hatanaka R, Hagiwara-Komoda Y, Kanamori Y, Fujita M, Okuda T, Kikawada T
  • Organizer: Cryobiology 2010
  • Place of Presentation: ブリストル、イギリス
  • Year and Date: 2010-07-18
• [Presentation] Molecular cloning and characterizaton of a novel LEA protein in an anhydrobiosis insect, Polypedilum vanderplanki (2010)
  • Author(s): Hatanaka R, Hagiwara-Komoda Y, Kanamori Y, Fujita M, Okuda T, Kikawada T
  • Organizer: Cryobiology 2010
  • Place of Presentation: ブリストル(イギリス)
  • Year and Date: 2010-07-18
• [Presentation] ネムリユスリカの極限乾燥耐性の分子機構 (2010)
  • Author(s): 黄川田隆洋
  • Organizer: 第9回バイオデミックフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2010-07-01
• [Presentation] ネムリユスリカの極限乾燥耐性の分子機構 (2010)
  • Author(s): 黄川田隆洋
  • Organizer: 第9回バイオテクノロジー国際会議,バイオアカデミックフォーラム
  • Place of Presentation: 東京ビックサイト
  • Year and Date: 2010-07-01
• [Presentation] ヨーロッパカブトエビ(Triops cancriformis)の休眠卵におけるトレハロース挙動 (2010)
  • Author(s): 柳下良平, 長島孝行, 奥田隆, 黄川田隆洋
  • Organizer: 第55回低温生物工学会年会セミナー
  • Place of Presentation: 東京工業大学
  • Year and Date: 2010-06-26
• [Presentation] ネムリユスリカの乾燥耐性と応答機構 (2010)
  • Author(s): 黄川田隆洋
  • Organizer: 第55回低温生物工学会年会セミナー
  • Place of Presentation: 東京
  • Year and Date: 2010-06-25
• [Presentation] ネムリユスリカの乾燥耐性と応答機構 (2010)
  • Author(s): 黄川田隆洋
  • Organizer: 第55回低温生物工学会年会セミナー
  • Place of Presentation: 東京工業大学
  • Year and Date: 2010-06-25
• [Presentation] 飢餓状態において脂肪体で発現するトビイロウンカ糖トランスポーターNIGLUT1の生理的意義 (2010)
  • Author(s): 菊田真吾、黄川田隆洋、萩原優香、中島信彦、野田博明
  • Organizer: 第54回日本応用動物昆虫学会大会
  • Place of Presentation: 千葉大学西千葉キャンパス
  • Year and Date: 2010-03-28
• [Presentation] ネムリユスリカの極限乾燥耐性 (2010)
  • Author(s): 黄川田隆洋、奥田隆
  • Organizer: 第54回日本応用動物昆虫学会大会 (招待講演)
  • Place of Presentation: 千葉
  • Year and Date: 2010-03-27
• [Presentation] ネムリユスリカの極限乾燥耐性 (2010)
  • Author(s): 黄川田隆洋、奥田隆
  • Organizer: 第54回日本応用動物昆虫学会大会
  • Place of Presentation: 千葉大学西千葉キャンパス
  • Year and Date: 2010-03-27
• [Presentation] ネムリユスリカの極限乾燥耐性を支える分子基盤の解明 (2009)
  • Author(s): 黄川田隆洋
  • Organizer: 極限環境微生物学会2009年度(第10回)年会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-29
• [Presentation] 乾燥耐性を持つ動物培細胞の作出 (2009)
  • Author(s): 中原雄一,今西重雄,光増可奈子,金森保志,岩田健一,渡邊匡彦,黄川田隆洋,奥田隆
  • Organizer: 極限環境微生物学会2009年度(第10回)年会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-29
• [Presentation] ネムリユスリカの極限乾燥耐性を支える分子基盤の解明(学会奨励賞受賞講演) (2009)
  • Author(s): 黄川田隆洋
  • Organizer: 極限環境微生物学会2009年度(第10回)年会
  • Place of Presentation: 明治大学駿河台キャンパス・アカデミーコモン
  • Year and Date: 2009-10-29
• [Presentation] 乾燥耐性を持つ動物培細胞の作出 (2009)
  • Author(s): 中原雄一, 今西重雄, 光増可奈子, 金森保志, 岩田健一, 渡邊匡彦, 黄川田隆洋, 奥田隆
  • Organizer: 極限環境微生物学会2009年度(第10回)年会
  • Place of Presentation: 明治大学駿河台キャンパス・アカデミーコモン
  • Year and Date: 2009-10-29
• [Presentation] Salt effects on the conformational structure of LEA Protein from Polypedilum vanderplanki and of its model peptide (2009)
  • Author(s): Furuki T, Shimizu T, Miyazawa M, Kikawada T, Okuda T, Sakurai M
  • Organizer: 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: 研究交流センター(茨城県つくば市)
  • Year and Date: 2009-08-25
• [Presentation] Model study of the desiccation-induced vitrification of group-3 late embryogenesis abundant proteins (2009)
  • Author(s): Furuki T, Shimizu T, Kikawada T, Okuda T, Takahashi T, Mihara H, Sakurai M
  • Organizer: 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: 研究交流センター(茨城県つくば市)
  • Year and Date: 2009-08-25
• [Presentation] Structural analysis for dehydrated LEA proteins of Polypedilum vanderplanki by replica exchange molecular dynamics simulation (2009)
  • Author(s): Furuki T, Miyama S, Shimizu T, Harano Y, Kanamori Y, Kikawada T, Okuda T, Sakurai M
  • Organizer: 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: 研究交流センター(茨城県つくば市)
  • Year and Date: 2009-08-25
• [Presentation] Regulation of trehalose metabolism underlying rapid accumulation of trehalose in the desiccating larvae of the sleeping chironomid (2009)
  • Author(s): Mitsumasu K, Kanamori Y, Fujita M, Iwata K, Tanaka D, Watanabe M, Kikawada T, Okuda T
  • Organizer: Polypedilum vanderplanki. 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: つくば
  • Year and Date: 2009-08-24
• [Presentation] Molecular bases of anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki (2009)
  • Author(s): Cornette R, 金森保志, Gusev O, 中原雄一, 藤田昭彦, 渡邊匡彦, 黄川田隆洋, 奥田隆
  • Organizer: 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: 研究交流センター(茨城県つくば市)
  • Year and Date: 2009-08-24
• [Presentation] Anhydrobiosis-associated changes in nuclear DNA and their effect on radioresistance in the sleeping chironomid Polypedilum vanderplanki (2009)
  • Author(s): Gusev O, Nakahara Y, Fujita A, Watanabe M, Kikawada T, Okuda T, Sakashita T, Funayama T, Hamada N, Wada S, Kobayashi Y
  • Organizer: 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: 研究交流センター(茨城県つくば市)
  • Year and Date: 2009-08-24
• [Presentation] Regulation of trehalose metabolism underlying rapid accumulation of trehalose in the desiccating larvae of the sleeping chironomid, Polypedilum vanderplanki (2009)
  • Author(s): Mitsumasu K, Kanamori Y, Fujita M, Iwata K, Tanaka D, Watanabe M, Kikawada T, Okuda T
  • Organizer: 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: 研究交流センター(茨城県つくば市)
  • Year and Date: 2009-08-24
• [Presentation] Three-dimensional structure and dynamics of trehalose transporter TRET1 in Polypedilum vandeplanki as revealed by computer simulations (2009)
  • Author(s): Okawa T, Kikawada T, Okuda T, Sakurai M
  • Organizer: 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: 研究交流センター(茨城県つくば市)
  • Year and Date: 2009-08-24
• [Presentation] Model study of the desiccation-induced vitrification of group-3 late embryogenesis abundant proteins (2009)
  • Author(s): Furuki T, Shimizu T, Kikawada T, Okuda T, Takahashi T, Mihara H, Sakurai M
  • Organizer: 46^<th> Annual Meeting of the Society for Cryobiology
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-23
• [Presentation] Salt effects on the conformational structure of LEA protein from Polypedilum vanderplanki and its model peptide (2009)
  • Author(s): Furuki T, Shimizu T, Miyazawa M, Kikawada T, Okuda T, Sakurai M
  • Organizer: 46^<th> Annual Meeting of the Society for Cryobiology
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-23
• [Presentation] The three-dimensional structure and dynamics of trehalose transporter TRET1 in Polypedilum vandeplanki-as revealed by computer simulations (2009)
  • Author(s): Okawa T, Kikawada T, Okuda T, Sakurai M
  • Organizer: 46^<th> Annual Meeting of the Society for Cryobiology
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-23
• [Presentation] Life without water: Anhydrobiosis in the sleeping chironomid (2009)
  • Author(s): Okuda T, Kikawada T, Sakurai M, Furuki T, Akao K, Nakahara Y, Oleg G, Saito A, Watanabe M, Iwata K, Kanamori Y, Cornette R
  • Organizer: Polypedilum vanderplanki. 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: 札幌
  • Year and Date: 2009-07-20
• [Presentation] Structural analysis for dehydrated LEA proteins of Polypedilum vanderplanki by replica exchange molecular dynamics simulation (2009)
  • Author(s): Miyama S, Shimizu T, Furuki T, Harano Y, Kanamori Y, Kikawada T, Okuda T, Sakurai M
  • Organizer: 46^<th> Annual Meeting of the Society for Cryobiology
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-20
• [Presentation] Life without water : Anhydrobiosis in the sleeping chironomid, Polypedilum vanderplanki (2009)
  • Author(s): Okuda T, Kikawada T, Sakurai M, Furuki T, Akao K, Nakahara Y, Oleg G, Saito A, Watanabe M, Iwata K, Kanamori Y, Cornette R
  • Organizer: 46^<th> Annual Meeting of the Society for Cryobiology
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-20
• [Presentation] ネムリユスリカの極限的な乾燥耐性の仕組みをさぐる (2009)
  • Author(s): 黄川田隆洋
  • Organizer: 日本大学 文理学部 総合文化研究室・自然科学研究所シンポジウム『動物の不思議を科学する』 (招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2009-06-20
• [Presentation] ネムリユスリカの極限的な乾燥耐性の仕組みをさぐる (2009)
  • Author(s): 黄川田隆洋
  • Organizer: 日本大学文理学部総合文化研究室・自然科学研究所シンポジウム『動物の不思議を科学する』
  • Place of Presentation: 日本大学文理学部
  • Year and Date: 2009-06-20
• [Presentation] 乾燥耐性生物ネムリユスリカの不思議な世界 (2009)
  • Author(s): 黄川田隆
  • Organizer: 極限環境微生物学会第10回シンポジウム (招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2009-06-09
• [Presentation] 乾燥耐性生物ネムリユスリカの不思議な世界 (2009)
  • Author(s): 黄川田隆洋
  • Organizer: 極限環境微生物学会
  • Place of Presentation: 東京大学農学部
  • Year and Date: 2009-06-09
• [Presentation] アフリカ産ネムリユスリカ:乾燥耐性の分子メカニズムを求めて (2009)
  • Author(s): Cornette R, 金森保志, Gusev O, 中原雄一, 藤田昭彦, 渡邊匡彦, 黄川田隆洋, 奥田隆
  • Organizer: 第20回ユスリカ研究集会
  • Place of Presentation: 国民宿舎-水郷(茨城県土浦市)
  • Year and Date: 2009-05-29
• [Presentation] ネの害虫トビイロウンカの Facilitative sugar transporter (2009)
  • Author(s): 菊田真吾, 萩原優香, 中島信彦, 黄川田隆洋, 野田博明
  • Organizer: 第4回トランスポーター研究会
  • Place of Presentation: 東京大学農学部
  • Year and Date: 2009-05-23
• [Presentation] Origin and functions of LEA proteins in an anhydrobiotic insect: insights from genomics and transcriptomics
  • Author(s): Gusev O, Hatanaka R, Cornette R, Okuda T, Furuki T, Sakurai M, Kikawada T
  • Organizer: NIAS International Seminar for Cryobiology and Cryotechnology
  • Place of Presentation: つくば
  • Invited
• [Presentation] 極限乾燥耐性昆虫ネムリユスリカとLEAタンパク質
  • Author(s): 畑中理恵, グセフ・オレグ, コルネット・リシャー, 末次克行, 奥田隆, 佐藤矩行, 黄川田隆洋
  • Organizer: 第57回低温生物工学会年会
  • Place of Presentation: つくば
• [Presentation] ネムリユスリカの極限乾燥耐性機構の分子基盤
  • Author(s): 黄川田隆洋
  • Organizer: 第57回低温生物工学会年会
  • Place of Presentation: つくば
  • Invited
• [Presentation] 活性酸素種によって制御される乾燥誘導性遺伝子の探索
  • Author(s): 迎恭輔, グセフ・オレグ, 畑中理恵, コルネット・リシャー, 奥田隆, 黄川田隆洋
  • Organizer: 第57回低温生物工学会年会
  • Place of Presentation: つくば
• [Presentation] Survival in extreme environment by ”preserve-expand-specialize” strategy: lessons from comparative genomics of an anhydrobiotic midge
  • Author(s): Gusev O, Sychev V, Novikova N, Sugimoto M, Okuda T, Kikawada T
  • Organizer: 39th COSPAR Scientific Assembly
  • Place of Presentation: マイソール、インド
  • Invited
• [Presentation] Morphological and transcriptional response of an anhydrobiotic insect to ionizing radiation and desiccation: steps forward in understanding molecular background of extreme radioresistance
  • Author(s): Gusev O, Mukae K, Sakashita T, Sychev V, Novikova N, Kikawada T, Okuda T
  • Organizer: 39th COSPAR Scientific Assembly
  • Place of Presentation: マイソール、インド
• [Presentation] Oxidative stress is a major factor for the induction of anhydrobiosis in the desiccation-tolerant midge, Polypedilum vanderplanki
  • Author(s): Cornette R, Iwata K, Kanamori Y, Gusev O, Kikawada T, Okuda T
  • Organizer: XXIV International Congress of Entomology
  • Place of Presentation: テグ、韓国
• [Presentation] Криптобиоз в насекомых: Генетические основы способности к выживанию в открытом космосе
  • Author(s): Гусев О.А, Кикавада Т, Окуда Т, Новикова Н.Д, Сычев В.Н, Левинских М.А
  • Organizer: Астробиология: от происхождения жизни на Земле к жизни во Вселенной
  • Place of Presentation: プシノ、ロシア
  • Invited
• [Presentation] ネムリユスリカの乾燥無代謝休眠を支えるゲノム情報と原因遺伝子の解明
  • Author(s): 末次克行, コルネット・リシャー, グセフ・オレグ, 川島武士, 佐藤矩行, 黄川田隆洋
  • Organizer: 新学術領域研究「複合適応形質進化の遺伝子基盤解明」平成24年度公開シンポジウム
  • Place of Presentation: 東京
• [Presentation] Surviving rules: wide overlap in gene expression response to desiccation and ionizing radiation in an anhydrobiotic midge
  • Author(s): Gusev O, Mukae K, Sugimoto M, Kikawada T, Sakashita T, Okuda T
  • Organizer: 12th European Workshop on Astrobiology
  • Place of Presentation: ストックホルム、スウェーデン
  • Invited
• [Presentation] メタボローム解析からみたネムリユスリカの乾燥耐性におけるトレハロースの役割
  • Author(s): 黄川田隆洋
  • Organizer: 第16回トレハロースシンポジウム
  • Place of Presentation: 東京
  • Invited
• [Presentation] Comparative genomics for understanding how to survive under extreme desiccation conditions
  • Author(s): Kikawada T, Gusev O, Suetsugu Y, Cornette R, Okuda T
  • Organizer: The Third International Scientific and Practical Conference: Postgenomic Methods of Analysis in Biology, and Laboratory and Clinical Medicine
  • Place of Presentation: カザニ、ロシア
  • Invited
• [Presentation] 極限乾燥耐性昆虫ネムリユスリカのLEAタンパク質によるSUMOプロテアーゼ阻害作用について
  • Author(s): 畑中理恵, 黄川田隆洋
  • Organizer: 2012年度極限環境生物学会年会
  • Place of Presentation: 東京
• [Presentation] Switching to survival mode: noncoding RNAs in an anhydrobiotic insect and their possible roles in acquiring complete desiccation resistance
  • Author(s): Gusev O, Cornette R, Suetsugu Y, Kikawada T
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] ネムリユスリカの乾燥無代謝休眠を支えるゲノム情報
  • Author(s): 黄川田隆洋, グセフオレグ, コルネットリシャー, 末次克行
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] ネムリユスリカの乾燥耐性における酸化ストレスの影響
  • Author(s): Cornette R, 岩田健一, 金森保志, Gusev O, 黄川田隆洋, 奥田隆
  • Organizer: 第57回日本応用動物昆虫学会大会
  • Place of Presentation: 藤沢
• [Presentation] ネムリユスリカのゲノム解析から見えてきた乾燥無代謝休眠の分子機構
  • Author(s): 黄川田隆洋, Oleg G, Cornette R, 末次克行
  • Organizer: 第57回日本応用動物昆虫学会大会
  • Place of Presentation: 藤沢
  • Invited
• [Book] 昆虫の低温耐性-その仕組みと調べ方-(担当した章 : トレハロース輸送活性) (2010)
  • Author(s): 薦田(萩原)優香,黄川田隆洋
  • Publisher: 岡山大学出版会
• [Book] 昆虫の低温耐性-その仕組みと調べ方-(担当した章:トレハロース輸送活性) (2010)
  • Author(s): 薦田(萩原)優香, 黄川田隆洋
  • Publisher: 岡山大学出版会
• [Remarks]
  • URL: http://www.nias.affrc.go.jp/anhydrobiosis
• [Remarks]
  • URL: http://www.nias.affrc.go.jp/anhydrobiosis
• [Remarks]
  • URL: http://www.nias.affrc.go.jp/anhydrobiosis/
• [Remarks]
  • URL: http://www.nias.affrc.go.jp/anhydrobiosis/