Ammonium nutrient dependent root development in plant

• Project/Area Number: 21688006
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Plant nutrition/Soil science
• Research Institution: Tohoku University
• Principal Investigator: KOJIMA Soichi 東北大学, 大学院・農学研究科, 助教 (30462683)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000); Fiscal Year 2011: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 窒素代謝 / 輸送担体 / 形態形成 / 植物栄養 / 根 / シグナル伝達 / アンモニウム / 遺伝的多様性

Research Abstract

Localized ammonium supply to Arabidopsis root increased root branching. The response was highly dependent on high-affinity ammonium transporter located at plasma membrane. This new knowledge may lead to improve nitrogen use efficiency in plant.

Research Products

• [Journal Article] Ammonium triggers lateral root branching in Arabidopsis in an AMT1 ; 3-dependent manner (2011)
  • Author(s): Lima J, Kojima S, Takahashi H and von Wiren N
  • Journal Title: Plant Cell Volume: 22 Pages: 3621-2633
  • Peer Reviewed
• [Journal Article] Disruption of a novel NADH-glutamate synthase2 gene caused marked reduction in spike let number of rice (2011)
  • Author(s): 田村亘, 小島創一, 豊川絢子, 渡邉英生, 田淵-小林真由美, 早川俊彦, 山谷知行
  • Journal Title: Frontiers in Plant Science Volume: vol.2 Pages: 57-57
  • DOI: 10.3389/fpls.2011.00057
  • Peer Reviewed
• [Journal Article] Reverse genetics approach to characterize a function of NADH-glutamate synthase1 in rice plants (2010)
  • Author(s): Tamura W, Hidaka Y, Tabuchi M, Kojima S, Hayakawa T, Sato T, Obara M, Kojima M, Sakakibara H and Yamaya T
  • Journal Title: Amino Acids Volume: 39 Pages: 1003-1012
  • NAID: 120006376372
  • Peer Reviewed
• [Journal Article] Ammonium triggers lateral root branching in Arabidopsis in an AMT1;3-dependent manner (2010)
  • Author(s): Lima, J., Kojima, S., Takahashi, H., von Wiren, N.
  • Journal Title: Plant Cell Volume: 22 Pages: 3621-3633
  • Peer Reviewed
• [Journal Article] Reverse genetics approach to characterize a function of NADH-glutamate synthase1 in rice plants (2010)
  • Author(s): W.Tamura, Y.Hidaka, M.Tabuchi, S.Kojima, T.Hayakawa, T.Sato, M.Obara, M.Kojima, H.Sakakibara, T.Yamaya
  • Journal Title: Amino Acids (未定, 印刷中)
  • NAID: 120006376372
  • Peer Reviewed
• [Presentation] Evidence to support a function of glutamine synthetase1 ; 2 in the primary assimilation of ammonium ions in rice roots (2012)
  • Author(s): Kazuhiro Funayama, Mayumi Tabuchi-Kobayashi, Yuki Sawa, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
• [Presentation] イネ根における新規プロテインキナーゼ(OsACTPK1)のアンモニウ吸収制御への関与 (2012)
  • Author(s): 谷合彰子, 澤勇己, 小原実広, 吉成晃, 小島創一, 山谷知行, 早川俊彦
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
• [Presentation] 酵母スプリットユビキチン法によるアンモニウム輸送担体と細胞質型グルタミン合成酵素の相互作用の解析 (2012)
  • Author(s): 松岡香矢, 大岩優貴, 早川俊彦, 山谷知行, 小島創一
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
• [Presentation] リン欠乏シロイヌナズナ根からのリンゴ酸およびクエン酸分泌に関わるトランスポーターの解析 (2011)
  • Author(s): 丸山隼人、佐々木孝行、小島創一、和崎淳
  • Organizer: 日本土壌肥料学会2011年度つくば大会
  • Place of Presentation: エポカルつくば
  • Year and Date: 2011-08-09
• [Presentation] Analysis of malate transporters induced in roots of phosphorus deficient or aluminum stressed Arabidopsis thaliana (2011)
  • Author(s): Maruyama, H., Sasaki, T., Kojima, S., Wasaki, J
  • Organizer: International Conference on Arabidopsis Research 2011
  • Place of Presentation: Madison, WI, USA
• [Presentation] Analysis of malate and citrate transporters induced in roots of phosphorus deficient Arabidopsis thaliana (2011)
  • Author(s): Hayato Maruyama, Takayuki Sasaki, Keiki Okazaki, Soichi Kojima, Takuro Shinano, Jun Wasaki
  • Organizer: Rhizosphere 3
  • Place of Presentation: Perth, Australia
• [Presentation] Reduction of the primary assimilation of ammnonium ions and panicle number in rice mutants lacking cytosolic glutamine synthetase1 ; 2 (2011)
  • Author(s): Kazuhiro Funayama, Mayumi Tabuchi-Kobayashi, Yuki Sawa, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya
  • Organizer: International Symposium "Strategies of Plants against Global Environmental Change"
  • Place of Presentation: Kurashiki, Okayama
• [Presentation] Interplay between ammonium transporter and glutamine synthethase in Arabidopsis (2011)
  • Author(s): Kaya Tanaka-Matsuoka, Yuki Oiwa, Toshihiko Hayakawa, Tomoyuki Yamaya, Soichi Kojima
  • Organizer: International Symposium "Strategies of Plants against Global Environmental Change"
  • Place of Presentation: Kurashiki, Okayama
• [Presentation] イネにおけるNADH依存性グルタミン酸合成酵素(NADH-GOGAT)1及び2遺伝子の破壊変異体の獲得とその表現型 (2010)
  • Author(s): 田村亘、豊川絢子、日高佑典、田渕真由美、小島創一、早川俊彦、山谷知行
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-20
• [Presentation] シロイヌナズナ高親和型尿素輸送担体の相互作用因子の探索 (2010)
  • Author(s): 吉田圭吾、大岩優貴、松岡香矢、早川俊彦、山谷知行、小島創一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-20
• [Presentation] シロイヌナズナ環境型のアンモニウム栄養に対する応答 (2010)
  • Author(s): 大岩優貴、佐々木和浩、吉田圭吾、松岡香矢、早川俊彦、佐藤雅志、山谷知行、小島創一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-20
• [Presentation] シロイヌナズナ根のアンモニウム栄養に対する応答の分子遺伝学的な解析 (2010)
  • Author(s): 小島創一、佐々木和浩、大岩優貴、松岡香矢、早川俊彦、佐藤雅志、山谷知行
  • Organizer: 日本土壌肥料学会2010年度札幌大会
  • Place of Presentation: 北海道大学
• [Presentation] Localized ammonium supply increases lateral root branching、Nitrogen2010 (2010)
  • Author(s): Lima J, Kojima S, Takahashi H and von Wiren N
  • Organizer: 1st International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: 犬山国際センター
• [Presentation] Interplay between ammonium transport and auxin signaling (2010)
  • Author(s): Kojima S, Oiwa Y, Schussler D, Yoshida K, Matsuoka K, Hayakawa T, Yamaya T, von Wiren N
  • Organizer: Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: 犬山国際センター
• [Presentation] Localized ammonium supply increases lateral root branching (2010)
  • Author(s): Joni Lima, Soichi Kojima, Hideki Takahashi, Nicolaus von Wiren
  • Organizer: Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: 犬山国際センター
• [Presentation] Reverse genetic approach unravels the physiological functions of NADH-dependent glutamate synthase1 (NADH-GOGAT1) in ammonium assimilation in the root and productivity of rice plant (2010)
  • Author(s): Wataru Tamura, Yusuke Hidaka, Mayumi Tabuchi, Soichi Kojima, Mikiko Kojima, Toshihiko Hayakawa, Hitoshi Sakakibara, Tomoyuki Yamaya
  • Organizer: Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: 犬山国際センター
• [Presentation] Cytosolic glutamine synthetase 1;2 is one of a key enzymes in ammonium assimilation in root of rice plant (2010)
  • Author(s): Kazuhiro Funayama, Mayumi Tabuchi, Yuki Sawa, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya
  • Organizer: Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: 犬山国際センター
• [Presentation] Changes of root system by polyamine supply is dependent on high-affinity urea transporter (2010)
  • Author(s): Keigo Yoshida, Yuki Oiwa, Kaya Tanaka-Matsuoka, Toshihiko Hayakawa, Tomoyuki Yamaya, Nicolaus von Wiren, Soichi Kojima
  • Organizer: Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: 犬山国際センター
• [Presentation] Rice vacuolar membrane-localized amino acid transporter, OsATA1;2, transports glutamine (2010)
  • Author(s): Toshihiko Hayakawa, Ryusuke Ishida, Keisuke Kobayashi, Kuni Sueyoshi, Toru Kudo, Yoko Shiroto, Yoshitsugu Hongo, Soichi Kojima, Tomoyuki Yamaya
  • Organizer: Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: 犬山国際センター
• [Presentation] Systemic and local root development to localized putrescine supply is dependent on high-affinity urea transporter (2010)
  • Author(s): Keigo Yoshida, Yuki Oiwa, Kaya Tanaka-Matsuoka, Toshihiko Hayakawa, Tomoyuki Yamaya, Nicolaus von Wiren, Soichi Kojima
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: パシフィコ横浜
• [Presentation] イネにおけるグルタミン情報伝達系への関与が期待される新規ACTドメインプロテインキナーゼ様タンパク質4のリン酸化能解析 (2010)
  • Author(s): 谷合彰子・澤勇己・西濱夏海・工藤徹・小島創一・山谷知行・早川俊彦
  • Organizer: The 2010 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition
  • Place of Presentation: 北海道大学
• [Presentation] サイトゾル型グルタミン合成酵素1;2遺伝子破壊がイネの収量に及ぼす影響 (2010)
  • Author(s): 舟山和宏・小林(田渕)真由美・澤勇己・小島創一・早川俊彦・山谷知行
  • Organizer: The 2010 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition
  • Place of Presentation: 北海道大学
• [Presentation] イネのNADH-グルタミン酸合成酵素2遺伝子破壊変異体を用いた表現型の解析 (2010)
  • Author(s): 田村亘・豊川絢子・田渕真由美・小島創一・早川俊彦・山谷知行
  • Organizer: The 2010 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition
  • Place of Presentation: 北海道大学
• [Presentation] シロイヌナズナ根のアンモニウム栄養に対する応答の分子遺伝学的な解析 (2010)
  • Author(s): 小島創一・佐々木和浩・大岩優貴・松岡香矢・早川俊彦・佐藤雅志・山谷知行
  • Organizer: The 2010 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition
  • Place of Presentation: 北海道大学
• [Presentation] シロイヌナズナ高親和型尿素輸送担体(AtDUR3)の翻訳後制御機構の探索とその基質特異性について (2010)
  • Author(s): 吉田圭吾・大岩優貴・松岡香矢・早川俊彦・山谷知行・小島創一
  • Organizer: The 2010 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition
  • Place of Presentation: 北海道大学
• [Presentation] シロイヌナズナにおけるアンモニウムの輸送とグルタミン代謝の協調 (2010)
  • Author(s): 小島創一、石山敬貴、早川俊彦、山谷知行、Nicolaus von Wiren、高橋秀樹
  • Organizer: 第五回トランスポーター研究会
  • Place of Presentation: 東京
• [Presentation] 植物の窒素栄養を運ぶトランスポーターの研究 (2009)
  • Author(s): 小島創一
  • Organizer: 第四回先端農学研究センターセミナー
  • Place of Presentation: 仙台
  • Year and Date: 2009-11-20
• [Presentation] シロイヌナズナの高親和尿素輸送担体の翻訳後制御機構の探索 (2009)
  • Author(s): 吉田圭吾, Anne Bohner, 早川俊彦, 山谷知行, Nicolaus von Wiren, 小島創一
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都
  • Year and Date: 2009-09-15
• [Presentation] イネ根におけるGlnセンサー候補OsACTPK遺伝子族の発現特性とOsACTPK4遺伝子破壊イネの解析 (2009)
  • Author(s): 澤勇己、西濱夏海、小島創一、山谷知行、早川俊彦
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都
  • Year and Date: 2009-09-15
• [Presentation] 液胞型アクアポリンの細胞膜アンモニウム輸送への寄与 (2009)
  • Author(s): 小島創一、早川俊彦、Nicolaus von Wiren、山谷知行
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都
  • Year and Date: 2009-09-15
• [Presentation] イネNADH依存性グルタミン酸合成酵素1(NADH-GOGAT1)破壊変異体の獲得と表現型の解析 (2009)
  • Author(s): 田村亘、日高佑典、田渕真由美、小島創一、早川俊彦、山谷知行
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都
  • Year and Date: 2009-09-15
• [Book] Nitrogen Assimilation in Plants, Research Signpost (2010)
  • Author(s): Soichi Kojima, Toshihiko Hayakawa, Nicolaus von Wiren, Tomoyuki Yamaya
  • Total Pages: 378
• [Book] Nitrogen Assimilation in Plants (2010)
  • Author(s): Soichi Kojima, Toshihiko Hayakawa, Nicolaus von Wiren, Tomoyuki Yamaya
  • Publisher: Research Signpost
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/cellbio/index-j.htm
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/cellbio/index-j.htm
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/cellbio/index-j.html